Restriction map for
pFN21AB5316
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 145 GCGG CCGC(-3/-1)
Alw44I 1274 GTGCAC G^TGCAC
ApaLI 1274 GTGCAC G^TGCAC
AspHI 1274 GTGCAC G[AT]GC[AT]^C
AvaII 1301 GGACC G^G[AT]CC
BbvI 279 GCAGC GCAGC(8/12)
BcgI 838 CGAACCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1227 TGATCA T^GATCA
BfaI 1291 CTAG C^TAG
BmyI 1274 GTGCAC G[AGT]GC[ACT]^C
BseRI 37 GAGGAG GAGGAG(10/8)
BsiHKAI 1274 GTGCAC G[AT]GC[AT]^C
Bsp1286I 1274 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 632 TGTACA T^GTACA
BsrDI 949 GCAATG GCAATG(2/0)
BsrGI 632 TGTACA T^GTACA
Cac8I 1181 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 1301 GGACC G^G[ACGT]CC
DraI 205 TTTAAA TTT^AAA
EaeI 1218 TGGCCG [CT]^GGCC[GA]
FbaI 1227 TGATCA T^GATCA
MaeI 1291 CTAG C^TAG
PacI 1105 TTAATTAA TTAAT^TAA
PleI 904 GACTC GAGTC(4/5)
PstI 403 CTGCAG CTGCA^G
SapI 1063 GAAGAGC GCTCTTC(1/4)
Sau96I 1301 GGACC G^G[ACGT]CC
ScaI 104 AGTACT AGT^ACT
SfaNI 442 GATGC GCATC(5/9)
SmlI 756 CTTGAG C^T[CT][GA]AG
SspBI 632 TGTACA T^GTACA
SspI 854 AATATT AAT^ATT
TseI 279 GCAGC G^C[AT]GC
XcmI 558 CCAGACTGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AseI 334 ATTAAT AT^TAAT
1104 ATTAAT
AsnI 334 ATTAAT AT^TAAT
1104 ATTAAT
Asp700I 314 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1000 GAAAAGATTC
BssKI 51 CCTGG ^CC[ACGT]GG
1326 CCAGG
BstNI 51 CCTGG CC^[AT]GG
1326 CCAGG
EarI 1063 GAAGAG CTCTTC(1/4)
1263 GAAGAG
EcoRI 372 GAATTC G^AATTC
409 GAATTC
EcoRII 51 CCTGG ^CC[AT]GG
1326 CCAGG
Fnu4HI 145 GCGGC GC^[ACGT]GC
279 GCAGC
HaeIII 915 GGCC GG^CC
1219 GGCC
HincII 243 GTTGAC GT[CT]^[GA]AC
1234 GTCAAC
HindII 243 GTTGAC GT[CT]^[GA]AC
1234 GTCAAC
HphI 184 GGTGA GGTGA(8/7)
766 GGTGA
ItaI 145 GCGGC GC^[ACGT]GC
279 GCAGC
Ksp632I 1063 GAAGAG CTCTTC(1/4)
1263 GAAGAG
MvaI 51 CCTGG CC^[AT]GG
1326 CCAGG
PshBI 334 ATTAAT AT^TAAT
1104 ATTAAT
ScrFI 51 CCTGG CC^[ACGT]GG
1326 CCAGG
SfcI 403 CTGCAG C^T[GA][CT]AG
1331 CTGTAG
TfiI 820 GAATC G^A[AT]TC
1005 GATTC
Tsp45I 307 GTCAC ^GT[GC]AC
681 GTCAC
XmnI 314 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1000 GAAAAGATTC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BsgI BsiYI BslI BsrI BstF5I
DdeI DpnII FokI HindIII HinfI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII RsaI Sau3AI Tru9I
Tsp509I TspRI