(Circular) (Six-base) MAPSORT of: pFN21AB5316.seq Check: 9405 from: 1 to: 6055 pFN21AB5316 6055 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 4118 5502 5770 1632 Size: 456 2030 1384 268 1917 Fragments arranged by size: 2030 1917 1384 456 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 3798 5370 3798 Size: 1572 4483 ApaLI G'TGCA_C Cuts at: 3279 4112 5268 3279 Size: 833 1156 4066 Fragments arranged by size: 4066 1156 833 ApoI r'AATT_y Cuts at: 1493 2377 2414 2535 2628 2774 2946 2962 2999 Size: 884 37 121 93 146 172 16 37 Cuts at: 2999 3319 3364 3590 3742 5851 1493 Size: 320 45 226 152 2109 1697 Fragments arranged by size: 2109 1697 884 320 226 172 152 146 121 93 45 37 37 16 AseI AT'TA_AT Cuts at: 160 1032 2340 3110 4609 160 Size: 872 1308 770 1499 1606 Fragments arranged by size: 1606 1499 1308 872 770 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3380 1870 Size: 73 1437 4545 Fragments arranged by size: 4545 1437 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3376 4831 618 Size: 325 217 627 1589 1455 1842 Fragments arranged by size: 1842 1589 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3374 1391 Size: 1983 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2832 2866 4246 4280 1903 Size: 34 895 34 1380 34 3678 Fragments arranged by size: 3678 1380 895 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 3232 1548 Size: 1684 4371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4666 136 Size: 107 122 71 631 605 2994 1525 Fragments arranged by size: 2994 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 3283 4116 5272 1790 Size: 1493 833 1156 2573 Fragments arranged by size: 2573 1493 1156 833 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 4744 442 Size: 416 53 83 399 24 2369 958 1753 Fragments arranged by size: 2369 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4715 1239 Size: 3476 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2782 4180 5045 5343 2782 Size: 1398 865 298 3494 Fragments arranged by size: 3494 1398 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4718 882 Size: 617 3219 2219 Fragments arranged by size: 3219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3862 4244 4879 275 Size: 53 83 186 936 2329 382 635 1451 Fragments arranged by size: 2329 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 1186 Size: 871 5184 BseYI C'CCAG_C Cuts at: 5258 5754 5258 Size: 496 5559 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2328 3215 3276 1163 Size: 573 592 887 61 3942 Fragments arranged by size: 3942 887 592 573 61 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3283 4116 4201 5272 3283 Size: 833 85 1071 4066 Fragments arranged by size: 4066 1071 833 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3283 3374 4116 4201 5272 1391 Size: 399 1493 91 742 85 1071 2174 Fragments arranged by size: 2174 1493 1071 742 399 91 85 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3410 844 Size: 2566 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2961 4550 4724 65 Size: 1183 1713 1589 174 1396 Fragments arranged by size: 1713 1589 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4699 4886 1700 Size: 110 2889 187 2869 Fragments arranged by size: 2889 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2637 96 Size: 2541 3514 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050 Size: 1413 367 385 17 1452 86 12 346 Cuts at: 6050 1972 Size: 1977 Fragments arranged by size: 1977 1452 1413 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5503 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2049 487 Size: 683 56 823 4493 Fragments arranged by size: 4493 823 683 56 BtsI GCAGTG_nn' Cuts at: 3203 3571 4365 4385 3203 Size: 368 794 20 4873 Fragments arranged by size: 4873 794 368 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 2212 3359 2212 Size: 1147 4908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3223 3502 4391 Size: 54 1425 50 275 18 1393 279 889 Cuts at: 4391 6020 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1393 889 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3063 3263 3985 1353 Size: 1710 200 722 3423 Fragments arranged by size: 3423 1710 722 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4642 5016 5162 387 Size: 1064 138 3053 374 146 1280 Fragments arranged by size: 3053 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 4118 4715 5502 5770 1239 Size: 393 456 2030 597 787 268 1524 Fragments arranged by size: 2030 1524 787 597 456 393 268 EcoRI G'AATT_C Cuts at: 2377 2414 3364 3742 2377 Size: 37 950 378 4690 Fragments arranged by size: 4690 950 378 37 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2257 2257 Size: 6055 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 5202 1678 Size: 3524 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 2345 2377 4696 Size: 32 135 32 491 32 250 32 2319 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2319 491 250 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2250 3241 3399 3645 669 Size: 988 281 312 991 158 246 3079 Fragments arranged by size: 3079 991 988 312 281 246 158 HindIII A'AGCT_T Cuts at: 748 2436 2486 2525 748 Size: 1688 50 39 4278 Fragments arranged by size: 4278 1688 50 39 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4233 Fragments arranged by size: 4233 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4013 4372 4531 518 Size: 648 2847 359 159 2042 Fragments arranged by size: 2847 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3471 4148 5296 5541 1606 Size: 1865 677 1148 245 2120 Fragments arranged by size: 2120 1865 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5503 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PacI TTA_AT'TAA Cuts at: 3114 3114 Size: 6055 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3104 3625 3104 Size: 521 5534 PstI C_TGCA'G Cuts at: 830 2412 3407 830 Size: 1582 995 3478 Fragments arranged by size: 3478 1582 995 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SapI GCTCTTCn'nnn_ Cuts at: 3063 3063 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 2111 4303 6015 2111 Size: 2192 1712 2151 Fragments arranged by size: 2192 2151 1712 SfcI C'TryA_G Cuts at: 826 1045 2408 3336 3403 4538 5219 5410 826 Size: 219 1363 928 67 1135 681 191 1471 Fragments arranged by size: 1471 1363 1135 928 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2761 4159 5060 5322 820 Size: 197 926 818 1398 901 262 1553 Fragments arranged by size: 1553 1398 926 901 818 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 2861 3979 5 Size: 47 2809 1118 2081 Fragments arranged by size: 2809 2081 1118 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 3494 513 Size: 552 2429 3074 Fragments arranged by size: 3074 2429 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2637 4301 Size: 274 80 33 51 1443 132 528 1664 Cuts at: 4301 6013 96 Size: 1712 138 Fragments arranged by size: 1712 1664 1443 528 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6015 6047 6015 Size: 32 6023 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2570 2011 Size: 559 5496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 2323 3009 4184 2323 Size: 686 1175 4194 Fragments arranged by size: 4194 1175 686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI