(Circular) (Six-base) MAPSORT of: pFN21AA0034.seq Check: 7150 from: 1 to: 9730 pFN21AA0034 9730 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7540 278 Size: 53 83 186 936 6004 2468 Fragments arranged by size: 6004 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4435 7073 1937 Size: 2498 2638 4594 Fragments arranged by size: 4594 2638 2498 Acc65I G'GTAC_C Cuts at: 2873 7051 2873 Size: 4178 5552 AclI AA'CG_TT Cuts at: 1563 2645 5305 7857 8230 1563 Size: 1082 2660 2552 373 3063 Fragments arranged by size: 3063 2660 2552 1082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2252 3105 3233 3308 3405 3734 3753 Size: 436 184 853 128 75 97 329 19 Cuts at: 3753 4500 4980 5226 6675 6834 7793 9177 9445 Size: 747 480 246 1449 159 959 1384 268 Cuts at: 9445 1632 Size: 1917 Fragments arranged by size: 1917 1449 1384 959 853 747 480 436 329 268 246 184 159 128 97 75 19 AflII C'TTAA_G Cuts at: 820 1017 4292 820 Size: 197 3275 6258 Fragments arranged by size: 6258 3275 197 AflIII A'CryG_T Cuts at: 1203 5117 5119 8629 9606 1203 Size: 3914 2 3510 977 1327 Fragments arranged by size: 3914 3510 1327 977 2 AgeI A'CCGG_T Cuts at: 8561 8561 Size: 9730 AhdI GACnn_n'nnGTC Cuts at: 8459 8459 Size: 9730 AleI CACnn'nnGTG Cuts at: 1166 5706 6303 1166 Size: 4540 597 4593 Fragments arranged by size: 4593 4540 597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3708 4898 1184 Size: 2524 1190 6016 Fragments arranged by size: 6016 2524 1190 AlwNI CAG_nnn'CTG Cuts at: 2017 2056 4039 4096 4976 6442 7473 9045 2017 Size: 39 1983 57 880 1466 1031 1572 2702 Fragments arranged by size: 2702 1983 1572 1466 1031 880 57 39 ApaLI G'TGCA_C Cuts at: 4184 7787 8943 4184 Size: 3603 1156 4971 Fragments arranged by size: 4971 3603 1156 ApoI r'AATT_y Cuts at: 1493 2111 3104 3174 4285 4599 4813 5225 5567 Size: 618 993 70 1111 314 214 412 342 Cuts at: 5567 5798 5981 6242 7039 7265 7417 9526 1493 Size: 231 183 261 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 1111 993 797 618 412 342 314 261 231 226 214 183 152 70 AseI AT'TA_AT Cuts at: 160 1032 4797 8284 160 Size: 872 3765 3487 1606 Fragments arranged by size: 3765 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9730 AvaI C'yCGr_G Cuts at: 1870 1943 5496 7055 1870 Size: 73 3553 1559 4545 Fragments arranged by size: 4545 3553 1559 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2044 2077 3244 3277 2044 Size: 33 1167 33 8497 Fragments arranged by size: 8497 1167 33 33 BamHI G'GATC_C Cuts at: 4151 4858 7060 7427 4151 Size: 707 2202 367 6454 Fragments arranged by size: 6454 2202 707 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2454 2873 6961 7051 8506 Size: 325 217 627 667 419 4088 90 1455 Cuts at: 8506 618 Size: 1842 Fragments arranged by size: 4088 1842 1455 667 627 419 325 217 90 BanII G_rGCy'C Cuts at: 1391 3838 5941 7049 1391 Size: 2447 2103 1108 4072 Fragments arranged by size: 4072 2447 2103 1108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 2746 3993 928 Size: 971 301 546 1247 6665 Fragments arranged by size: 6665 1247 971 546 301 BbvCI CC'TCA_GC Cuts at: 4545 4545 Size: 9730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7921 7955 1903 Size: 34 5984 34 3678 Fragments arranged by size: 5984 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3272 3524 7622 8832 3272 Size: 252 4098 1210 4170 Fragments arranged by size: 4170 4098 1210 252 BclI T'GATC_A Cuts at: 1548 5901 1548 Size: 4353 5377 BfrBI ATG'CAT Cuts at: 3775 3775 Size: 9730 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8341 136 Size: 107 122 71 631 605 6669 1525 Fragments arranged by size: 6669 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9725 9725 Size: 9730 BlpI GC'TnA_GC Cuts at: 6492 7147 6492 Size: 655 9075 Bme1580I G_kGCm'C Cuts at: 1790 4188 7791 8947 1790 Size: 2398 3603 1156 2573 Fragments arranged by size: 3603 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9730 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3401 4227 5341 Size: 416 53 83 399 24 1984 826 1114 Cuts at: 5341 5461 5933 6910 6933 7461 8419 442 Size: 120 472 977 23 528 958 1753 Fragments arranged by size: 1984 1753 1114 977 958 826 528 472 416 399 120 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 2235 2604 1056 Size: 1179 369 8182 Fragments arranged by size: 8182 1179 369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2033 2413 2697 2748 3171 3522 5048 Size: 788 6 380 284 51 423 351 1526 Cuts at: 5048 5347 6032 8390 1239 Size: 299 685 2358 2579 Fragments arranged by size: 2579 2358 1526 788 685 423 380 351 299 284 51 6 Bpu10I CC'TnA_GC Cuts at: 4272 4545 4639 4272 Size: 273 94 9363 Fragments arranged by size: 9363 273 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3768 3779 5951 7855 8720 9018 2885 Size: 883 11 2172 1904 865 298 3597 Fragments arranged by size: 3597 2172 1904 883 865 298 11 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4139 4900 8393 882 Size: 617 2640 761 3493 2219 Fragments arranged by size: 3493 2640 2219 761 617 BsaAI yAC'GTr Cuts at: 493 1204 3065 5120 7521 493 Size: 711 1861 2055 2401 2702 Fragments arranged by size: 2702 2401 2055 1861 711 BsaBI GATnn'nnATC Cuts at: 4746 9724 4746 Size: 4978 4752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7537 7919 8554 275 Size: 53 83 186 936 6004 382 635 1451 Fragments arranged by size: 6004 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5220 8163 8561 8835 8982 9393 5220 Size: 2943 398 274 147 411 5557 Fragments arranged by size: 5557 2943 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3341 3371 1178 Size: 30 165 30 1938 30 7537 Fragments arranged by size: 7537 1938 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3231 4631 1186 Size: 2045 1400 6285 Fragments arranged by size: 6285 2045 1400 BseYI C'CCAG_C Cuts at: 2068 2255 3217 3222 8933 9429 2068 Size: 187 962 5 5711 496 2369 Fragments arranged by size: 5711 2369 962 496 187 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2502 2907 3939 6327 1163 Size: 573 766 405 1032 2388 4566 Fragments arranged by size: 4566 2388 1032 766 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7180 7941 8969 1540 Size: 275 186 5179 761 1028 2301 Fragments arranged by size: 5179 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4188 4371 5941 6009 7791 7876 8947 4188 Size: 183 1570 68 1782 85 1071 4971 Fragments arranged by size: 4971 1782 1570 1071 183 85 68 BsmI GAATG_Cn' Cuts at: 3695 7241 7334 3695 Size: 3546 93 6091 Fragments arranged by size: 6091 3546 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3838 4188 4371 5941 6009 7049 7791 Size: 399 2048 350 183 1570 68 1040 742 Cuts at: 7791 7876 8947 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2048 1570 1071 1040 742 399 350 183 85 68 BspEI T'CCGG_A Cuts at: 5220 9393 5220 Size: 4173 5557 BspHI T'CATG_A Cuts at: 2220 2929 4072 4603 4989 6810 7619 9349 2220 Size: 709 1143 531 386 1821 809 1730 2601 Fragments arranged by size: 2601 1821 1730 1143 809 709 531 386 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2885 5326 7085 844 Size: 2041 2441 1759 3489 Fragments arranged by size: 3489 2441 2041 1759 BsrBI CCG'CTC Cuts at: 1394 1823 7617 1394 Size: 429 5794 3507 Fragments arranged by size: 5794 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 4813 5194 5317 6621 8225 8399 Size: 1183 1551 2014 381 123 1304 1604 174 Cuts at: 8399 65 Size: 1396 Fragments arranged by size: 2014 1604 1551 1396 1304 1183 381 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 3077 8374 8561 1700 Size: 110 1267 5297 187 2869 Fragments arranged by size: 5297 2869 1267 187 110 BsrGI T'GTAC_A Cuts at: 96 4414 4927 5655 5836 96 Size: 4318 513 728 181 3990 Fragments arranged by size: 4318 3990 728 513 181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9730 BssSI C'ACGA_G Cuts at: 6303 7790 8802 6303 Size: 1487 1012 7231 Fragments arranged by size: 7231 1487 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9730 BstBI TT'CG_AA Cuts at: 2191 8534 2191 Size: 6343 3387 BstEII G'GTnAC_C Cuts at: 1184 5609 1184 Size: 4425 5305 BstXI CCAn_nnnn'nTGG Cuts at: 4869 5606 4869 Size: 737 8993 BstYI r'GATC_y Cuts at: 1972 2343 4151 4741 4858 7060 7427 7812 7829 Size: 371 1808 590 117 2202 367 385 17 Cuts at: 7829 9281 9367 9379 9725 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2202 1977 1808 1452 590 385 371 367 346 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3443 3443 Size: 9730 BtgI C'CryG_G Cuts at: 513 1065 2004 6287 513 Size: 552 939 4283 3956 Fragments arranged by size: 4283 3956 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8991 Fragments arranged by size: 8991 683 56 BtsI GCAGTG_nn' Cuts at: 2751 3692 3927 5094 6674 7246 8040 8060 2751 Size: 941 235 1167 1580 572 794 20 4421 Fragments arranged by size: 4421 1580 1167 941 794 572 235 20 ClaI AT'CG_AT Cuts at: 1554 4342 1554 Size: 2788 6942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5425 5460 529 Size: 35 4861 35 4799 Fragments arranged by size: 4861 4799 35 35 DraI TTT'AAA Cuts at: 4192 7034 4192 Size: 2842 6888 DraIII CAC_nnn'GTG Cuts at: 3434 3434 Size: 9730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2101 8737 809 Size: 507 785 6636 1802 Fragments arranged by size: 6636 1802 785 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4774 7177 8066 Size: 54 1425 50 275 18 2944 2403 889 Cuts at: 8066 9695 8 Size: 1629 43 Fragments arranged by size: 2944 2403 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7177 1537 Size: 275 5365 4090 Fragments arranged by size: 5365 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 3618 4730 4826 5889 6551 7660 1353 Size: 918 1347 1112 96 1063 662 1109 3423 Fragments arranged by size: 3423 1347 1112 1109 1063 918 662 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8317 8691 8837 387 Size: 1064 138 6728 374 146 1280 Fragments arranged by size: 6728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5939 5939 Size: 9730 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2033 2068 2252 2413 2697 2748 Size: 393 395 6 35 184 161 284 51 Cuts at: 2748 3105 3171 3233 3308 3405 3522 3734 3753 Size: 357 66 62 75 97 117 212 19 Cuts at: 3753 4500 4980 5048 5226 5347 6032 6675 6834 Size: 747 480 68 178 121 685 643 159 Cuts at: 6834 7793 8390 9177 9445 1239 Size: 959 597 787 268 1524 Fragments arranged by size: 1524 959 787 747 685 643 597 480 395 393 357 284 268 212 184 178 161 159 121 117 97 75 68 66 62 51 35 19 6 EcoNI CCTnn'n_nnAGG Cuts at: 6605 6605 Size: 9730 EcoO109I rG'GnC_Cy Cuts at: 3737 4174 4954 6935 3737 Size: 437 780 1981 6532 Fragments arranged by size: 6532 1981 780 437 EcoRI G'AATT_C Cuts at: 3174 4599 4813 5798 6242 7039 7417 3174 Size: 1425 214 985 444 797 378 5487 Fragments arranged by size: 5487 1425 985 797 444 378 214 EcoRV GAT'ATC Cuts at: 6184 6184 Size: 9730 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3647 4914 6089 6617 6838 3647 Size: 1267 1175 528 221 6539 Fragments arranged by size: 6539 1267 1175 528 221 FspI TGC'GCA Cuts at: 8236 8236 Size: 9730 HaeII r_GCGC'y Cuts at: 1678 2217 8877 1678 Size: 539 6660 2531 Fragments arranged by size: 6660 2531 539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5393 5425 6130 6162 6467 Size: 32 135 32 3821 32 705 32 305 Cuts at: 6467 6499 8371 8403 8445 8477 1373 Size: 32 1872 32 42 32 2626 Fragments arranged by size: 3821 2626 1872 705 305 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3961 4086 4162 4436 4471 5333 Size: 988 281 2023 125 76 274 35 862 Cuts at: 5333 7074 7320 669 Size: 1741 246 3079 Fragments arranged by size: 3079 2023 1741 988 862 281 274 246 125 76 35 HindIII A'AGCT_T Cuts at: 748 748 Size: 9730 HpaI GTT'AAC Cuts at: 4162 7320 4162 Size: 3158 6572 KpnI G_GTAC'C Cuts at: 2877 7055 2877 Size: 4178 5552 MfeI C'AATT_G Cuts at: 7329 7329 Size: 9730 MluI A'CGCG_T Cuts at: 9606 9606 Size: 9730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3508 4699 5623 5892 6173 6273 8844 9028 Size: 2203 1191 924 269 281 100 2571 184 Cuts at: 9028 1305 Size: 2007 Fragments arranged by size: 2571 2203 2007 1191 924 281 269 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 4776 10 Size: 54 1425 343 2944 4964 Fragments arranged by size: 4964 2944 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2285 2856 2885 5706 5844 5948 Size: 648 999 120 571 29 2821 138 104 Cuts at: 5948 6303 7688 8047 8206 518 Size: 355 1385 359 159 2042 Fragments arranged by size: 2821 2042 1385 999 648 571 359 355 159 138 120 104 29 MspA1I CmG'CkG Cuts at: 1606 2942 5723 7146 7823 8971 9216 1606 Size: 1336 2781 1423 677 1148 245 2120 Fragments arranged by size: 2781 2120 1423 1336 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 8239 Fragments arranged by size: 8239 939 552 NdeI CA'TA_TG Cuts at: 387 5290 387 Size: 4903 4827 NheI G'CTAG_C Cuts at: 1052 2231 2600 1052 Size: 1179 369 8182 Fragments arranged by size: 8182 1179 369 NotI GC'GGCC_GC Cuts at: 7177 7177 Size: 9730 NsiI A_TGCA'T Cuts at: 3777 3777 Size: 9730 NspI r_CATG'y Cuts at: 1132 1229 2597 3779 5843 7088 8633 1132 Size: 97 1368 1182 2064 1245 1545 2229 Fragments arranged by size: 2229 2064 1545 1368 1245 1182 97 PasI CC'CwG_GG Cuts at: 1348 3124 1348 Size: 1776 7954 PciI A'CATG_T Cuts at: 8629 8629 Size: 9730 PflMI CCAn_nnn'nTGG Cuts at: 1108 2056 4363 6995 7010 1108 Size: 948 2307 2632 15 3828 Fragments arranged by size: 3828 2632 2307 948 15 PmeI GTTT'AAAC Cuts at: 7034 7034 Size: 9730 PmlI CAC'GTG Cuts at: 5120 5120 Size: 9730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7799 7831 9312 9344 7799 Size: 32 1481 32 8185 Fragments arranged by size: 8185 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3737 4174 4954 6935 3737 Size: 437 780 1981 6532 Fragments arranged by size: 6532 1981 780 437 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9730 PsiI TTA'TAA Cuts at: 7300 7300 Size: 9730 PstI C_TGCA'G Cuts at: 830 3876 4044 4999 6061 6669 7082 830 Size: 3046 168 955 1062 608 413 3478 Fragments arranged by size: 3478 3046 1062 955 608 413 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9730 PvuII CAG'CTG Cuts at: 2942 5723 2942 Size: 2781 6949 SacI G_AGCT'C Cuts at: 5941 5941 Size: 9730 SalI G'TCGA_C Cuts at: 1936 4434 7072 1936 Size: 2498 2638 4594 Fragments arranged by size: 4594 2638 2498 SapI GCTCTTCn'nnn_ Cuts at: 3618 5889 3618 Size: 2271 7459 SbfI CC_TGCA'GG Cuts at: 7082 7082 Size: 9730 ScaI AGT'ACT Cuts at: 3254 5471 5752 6821 7978 9690 3254 Size: 2217 281 1069 1157 1712 3294 Fragments arranged by size: 3294 2217 1712 1157 1069 281 SexAI A'CCwGG_T Cuts at: 4842 4920 4842 Size: 78 9652 SfcI C'TryA_G Cuts at: 826 1045 2559 2715 3872 4040 4246 4443 4995 Size: 219 1514 156 1157 168 206 197 552 Cuts at: 4995 6057 6217 6665 6882 7078 8213 8894 9085 Size: 1062 160 448 217 196 1135 681 191 Cuts at: 9085 826 Size: 1471 Fragments arranged by size: 1514 1471 1157 1135 1062 681 552 448 219 217 206 197 196 191 168 160 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9730 SmaI CCC'GGG Cuts at: 1872 7057 1872 Size: 5185 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2864 3758 3783 4292 5496 5930 Size: 197 926 921 894 25 509 1204 434 Cuts at: 5930 7834 8735 8997 820 Size: 1904 901 262 1553 Fragments arranged by size: 1904 1553 1204 926 921 901 894 509 434 262 197 25 SnaBI TAC'GTA Cuts at: 493 3065 7521 493 Size: 2572 4456 2702 Fragments arranged by size: 4456 2702 2572 SpeI A'CTAG_T Cuts at: 152 5441 152 Size: 5289 4441 SphI G_CATG'C Cuts at: 1132 3779 7088 1132 Size: 2647 3309 3774 Fragments arranged by size: 3774 3309 2647 SspI AAT'ATT Cuts at: 5 52 6037 7654 5 Size: 47 5985 1617 2081 Fragments arranged by size: 5985 2081 1617 47 StuI AGG'CCT Cuts at: 1517 3316 1517 Size: 1799 7931 StyI C'CwwG_G Cuts at: 513 1065 2004 4583 6042 6192 7169 513 Size: 552 939 2579 1459 150 977 3074 Fragments arranged by size: 3074 2579 1459 977 939 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3947 5696 7757 7774 7927 8112 Size: 1255 1803 564 1749 2061 17 153 185 Cuts at: 8112 8557 325 Size: 445 1498 Fragments arranged by size: 2061 1803 1749 1498 1255 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 3319 3689 Size: 274 80 33 51 1443 1275 67 370 Cuts at: 3689 4414 4927 5469 5655 5750 5836 6819 7976 Size: 725 513 542 186 95 86 983 1157 Cuts at: 7976 9688 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1275 1157 983 725 542 513 370 274 186 138 95 86 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6559 6591 9690 9722 6559 Size: 32 3099 32 6567 Fragments arranged by size: 6567 3099 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4352 1316 Size: 3036 6694 XbaI T'CTAG_A Cuts at: 6026 7066 6026 Size: 1040 8690 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6156 6965 6156 Size: 809 8921 XhoI C'TCGA_G Cuts at: 1943 5496 1943 Size: 3553 6177 XmaI C'CCGG_G Cuts at: 1870 7055 1870 Size: 5185 4545 XmnI GAAnn'nnTTC Cuts at: 5424 5798 7859 5424 Size: 374 2061 7295 Fragments arranged by size: 7295 2061 374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7538 276 Size: 53 83 186 936 6004 2468 Fragments arranged by size: 6004 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI