Restriction map for
pFN21AE0566
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AlwNI AocI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI BstYI
Bsu36I CelII ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MscI MslI MspA1I MunI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfuI SnaBI
SpeI Sse8387I StyI Tsp45I Tth111I
XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 124 TCCGGA T^CCGGA
AflIII 716 ACATGT A^C[GA][CT]GT
Alw44I 1096 GTGCAC G^TGCAC
AlwI 346 GATCC GGATC(4/5)
Aor51HI 320 AGCGCT AGC^GCT
ApaI 230 GGGCCC GGGCC^C
ApaLI 1096 GTGCAC G^TGCAC
Asp700I 424 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1096 GTGCAC G[AT]GC[AT]^C
BanII 230 GGGCCC G[GA]GC[CT]^C
BpmI 32 CTGGAG CTGGAG(16/14)
BsaWI 124 TCCGGA [AT]^CCGG[AT]
BseAI 124 TCCGGA T^CCGGA
BseRI 91 CTCCTC GAGGAG(10/8)
BsgI 138 GTGCAG GTGCAG(16/14)
BsiHKAI 1096 GTGCAC G[AT]GC[AT]^C
Bsp1407I 577 TGTACA T^GTACA
BspEI 124 TCCGGA T^CCGGA
BspLU11I 716 ACATGT A^CATGT
BspMI 11 ACCTGC ACCTGC(4/8)
BsrGI 577 TGTACA T^GTACA
Bst1107I 366 GTATAC GTA^TAC
BstZ17I 366 GTATAC GTA^TAC
DdeI 362 CTAAG C^T[ACGT]AG
DpnII 346 GATC ^GATC
DraI 457 TTTAAA TTT^AAA
EaeI 304 TGGCCG [CT]^GGCC[GA]
Eco47III 320 AGCGCT AGC^GCT
EcoT22I 493 ATGCAT ATGCA^T
MboI 346 GATC ^GATC
MboII 1177 GAAGA GAAGA(8/7)
MroI 124 TCCGGA T^CCGGA
NdeI 1172 CATATG CA^TATG
NdeII 346 GATC ^GATC
NsiI 493 ATGCAT ATGCA^T
PaeR7I 114 CTCGAG C^TCGAG
PflMI 25 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 856 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 328 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 346 GATC ^GATC
ScaI 130 AGTACT AGT^ACT
SgrAI 98 CACCGGCG C[GA]^CCGG[CT]G
SmaI 272 CCCGGG CCC^GGG
SmlI 114 CTCGAG C^T[CT][GA]AG
SphI 86 GCATGC GCATG^C
SspBI 577 TGTACA T^GTACA
StuI 110 AGGCCT AGG^CCT
SwaI 456 ATTTAAAT ATTT^AAAT
Van91I 25 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 114 CTCGAG C^TCGAG
XmaCI 272 CCCGGG C^CCGGG
XmaI 272 CCCGGG C^CCGGG
XmnI 424 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 160 GTAGAC GT^[AC][GT]AC
366 GTATAC
AvaII 269 GGTCC G^G[AT]CC
857 GGACC
BsaI 467 GAGACC GGTCTC(1/5)
276 GGTCTC
BsrFI 99 ACCGGC [GA]^CCGG[CT]
306 GCCGGT
BstF5I 285 CATCC GGATG(2/0)
606 GGATG
Cfr10I 99 ACCGGC [GA]^CCGG[CT]
306 GCCGGT
DraII 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
856 AGGACCT
EcoO109I 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
856 AGGACCT
FokI 285 CATCC GGATG(9/13)
606 GGATG
NspI 86 GCATGC [GA]CATG^[CT]
716 ACATGT
PleI 993 GACTC GAGTC(4/5)
1065 GAGTC
SspI 921 AATATT AAT^ATT
1130 AATATT
TaqI 115 TCGA T^CGA
432 TCGA
TfiI 509 GATTC G^A[AT]TC
826 GATTC
TthHB8I 115 TCGA T^CGA
432 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AvaI BanI BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MnlI MseI
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfoI Tru9I TseI
Tsp509I TspRI