(Circular) (Six-base) MAPSORT of: pFN21AE0566.seq Check: 8075 from: 1 to: 5926 pFN21AE0566 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 2372 3269 1937 Size: 229 206 897 4594 Fragments arranged by size: 4594 897 229 206 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AfeI AGC'GCT Cuts at: 2327 2327 Size: 5926 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2721 4825 5802 1203 Size: 1518 2104 977 1327 Fragments arranged by size: 2104 1518 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 2239 2239 Size: 5926 ApaLI G'TGCA_C Cuts at: 3101 3983 5139 3101 Size: 882 1156 3888 Fragments arranged by size: 3888 1156 882 ApoI r'AATT_y Cuts at: 1493 2459 2769 2849 2882 3059 3081 3155 3235 Size: 966 310 80 33 177 22 74 80 Cuts at: 3235 3461 3613 5722 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 966 310 226 177 152 80 80 74 33 22 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2211 2277 2284 3251 1870 Size: 73 176 92 66 7 967 4545 Fragments arranged by size: 4545 967 176 92 73 66 7 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2180 2301 2314 3247 Size: 325 217 627 273 120 121 13 933 Cuts at: 3247 4702 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 933 627 325 273 217 121 120 13 BanII G_rGCy'C Cuts at: 1391 2239 3245 1391 Size: 848 1006 4072 Fragments arranged by size: 4072 1006 848 BbeI G_GCGC'C Cuts at: 2064 2184 2305 2064 Size: 120 121 5685 Fragments arranged by size: 5685 121 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4117 4151 1903 Size: 34 50 34 2096 34 3678 Fragments arranged by size: 3678 2096 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BfrBI ATG'CAT Cuts at: 2500 2500 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2239 2319 3105 3987 5143 1790 Size: 449 80 786 882 1156 2573 Fragments arranged by size: 2573 1156 882 786 449 80 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2148 2396 2444 Size: 416 53 83 399 24 731 248 48 Cuts at: 2444 3657 4615 442 Size: 1213 958 1753 Fragments arranged by size: 1753 1213 958 731 416 399 248 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 4586 1239 Size: 819 2528 2579 Fragments arranged by size: 2579 2528 819 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2287 2466 4589 882 Size: 617 788 179 2123 2219 Fragments arranged by size: 2219 2123 788 617 179 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2108 2181 2302 Size: 53 83 186 936 528 47 73 121 Cuts at: 2302 3733 4115 4750 275 Size: 1431 382 635 1451 Fragments arranged by size: 1451 1431 936 635 528 382 186 121 83 73 53 47 BsaWI w'CCGG_w Cuts at: 2129 4359 4757 5031 5178 5589 2129 Size: 2230 398 274 147 411 2466 Fragments arranged by size: 2466 2230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 1186 Size: 901 5025 BseYI C'CCAG_C Cuts at: 2232 5129 5625 2232 Size: 2897 496 2533 Fragments arranged by size: 2897 2533 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2164 1163 Size: 573 428 4925 Fragments arranged by size: 4925 573 428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3105 3987 4072 5143 3105 Size: 882 85 1071 3888 Fragments arranged by size: 3888 1071 882 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2319 3105 3245 3987 4072 5143 Size: 399 449 80 786 140 742 85 1071 Cuts at: 5143 1391 Size: 2174 Fragments arranged by size: 2174 1071 786 742 449 399 140 85 80 BspEI T'CCGG_A Cuts at: 2129 5589 2129 Size: 3460 2466 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 3281 844 Size: 1181 1256 3489 Fragments arranged by size: 3489 1256 1181 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 2311 4570 4757 1700 Size: 110 294 207 2259 187 2869 Fragments arranged by size: 2869 2259 294 207 187 110 BsrGI T'GTAC_A Cuts at: 96 2582 96 Size: 2486 3440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5926 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921 Size: 1284 367 385 17 1452 86 12 346 Cuts at: 5921 1972 Size: 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2373 2373 Size: 5926 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5374 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3099 3442 4236 4256 3099 Size: 343 794 20 4769 Fragments arranged by size: 4769 794 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 2464 3230 2464 Size: 766 5160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2309 3373 4262 Size: 54 1425 50 275 18 479 1064 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1064 889 479 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3856 1353 Size: 2503 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 3989 4586 5373 5641 1239 Size: 393 426 1931 597 787 268 1524 Fragments arranged by size: 1931 1524 787 597 426 393 268 EcoO109I rG'GnC_Cy Cuts at: 2236 2862 2236 Size: 626 5300 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2064 2184 2246 2305 2329 2640 5073 1678 Size: 386 120 62 59 24 311 2433 2531 Fragments arranged by size: 2531 2433 386 311 120 62 59 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373 Size: 32 135 32 2995 32 42 32 2626 Fragments arranged by size: 2995 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2060 2180 2301 2060 Size: 120 121 5685 Fragments arranged by size: 5685 121 120 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2408 2593 2623 5040 5224 1305 Size: 1103 185 30 2417 184 2007 Fragments arranged by size: 2417 2007 1103 185 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3884 4243 4402 518 Size: 648 2718 359 159 2042 Fragments arranged by size: 2718 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3342 4019 5167 5412 1606 Size: 1736 677 1148 245 2120 Fragments arranged by size: 2120 1736 1148 677 245 NarI GG'CG_CC Cuts at: 2061 2181 2302 2061 Size: 120 121 5685 Fragments arranged by size: 5685 121 120 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 3178 387 Size: 2791 3135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NsiI A_TGCA'T Cuts at: 2502 2502 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2095 2725 3284 4829 1132 Size: 97 866 630 559 1545 2229 Fragments arranged by size: 2229 1545 866 630 559 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 2721 4825 2721 Size: 2104 3822 PflMI CCAn_nnn'nTGG Cuts at: 1108 2036 1108 Size: 928 4998 PfoI T'CCnGG_A Cuts at: 2071 2071 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2862 2862 Size: 5926 PshAI GACnn'nnGTC Cuts at: 1613 2337 1613 Size: 724 5202 PsiI TTA'TAA Cuts at: 3126 3496 3126 Size: 370 5556 PspOMI G'GGCC_C Cuts at: 2235 2235 Size: 5926 PstI C_TGCA'G Cuts at: 830 3278 830 Size: 2448 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 2137 4174 5886 2137 Size: 2037 1712 2177 Fragments arranged by size: 2177 2037 1712 SfcI C'TryA_G Cuts at: 826 1045 3274 4409 5090 5281 826 Size: 219 2229 1135 681 191 1471 Fragments arranged by size: 2229 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2062 2182 2303 2062 Size: 120 121 5685 Fragments arranged by size: 5685 121 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 2104 1700 Size: 404 5522 SmaI CCC'GGG Cuts at: 1872 2279 3253 1872 Size: 407 974 4545 Fragments arranged by size: 4545 974 407 SmlI C'TyrA_G Cuts at: 820 1017 1943 2119 4030 4931 5193 820 Size: 197 926 176 1911 901 262 1553 Fragments arranged by size: 1911 1553 926 901 262 197 176 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2095 3284 1132 Size: 963 1189 3774 Fragments arranged by size: 3774 1189 963 SspI AAT'ATT Cuts at: 5 52 2928 3137 3850 5 Size: 47 2876 209 713 2081 Fragments arranged by size: 2876 2081 713 209 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5326 StyI C'CwwG_G Cuts at: 513 1065 3365 513 Size: 552 2300 3074 Fragments arranged by size: 3074 2300 552 SwaI ATTT'AAAT Cuts at: 2464 2464 Size: 5926 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 2734 3953 3970 4123 4308 4753 Size: 1255 550 604 1219 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 1219 604 550 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2135 2522 2582 Size: 274 80 33 51 1443 158 387 60 Cuts at: 2582 4172 5884 96 Size: 1590 1712 138 Fragments arranged by size: 1712 1590 1443 387 274 158 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 2119 1943 Size: 176 5750 XmaI C'CCGG_G Cuts at: 1870 2277 3251 1870 Size: 407 974 4545 Fragments arranged by size: 4545 974 407 XmnI GAAnn'nnTTC Cuts at: 2433 4055 2433 Size: 1622 4304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI XcmI