Restriction map for
pFN21AE0566
(Linear) (Six-base) MAPSORT of: KIEE0566.seq Check: 1502 from: 1 to: 1221
KIEE0566.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 161 367 1221
Size: 161 206 854
Fragments arranged by size:
854 206 161
AfeI AGC'GCT
Cuts at: 0 322 1221
Size: 322 899
AflIII A'CryG_T
Cuts at: 0 716 1221
Size: 716 505
ApaI G_GGCC'C
Cuts at: 0 234 1221
Size: 234 987
ApaLI G'TGCA_C
Cuts at: 0 1096 1221
Size: 1096 125
ApoI r'AATT_y
Cuts at: 0 454 764 844 877 1054 1076 1150 1221
Size: 454 310 80 33 177 22 74 71
Fragments arranged by size:
454 310 177 80 74 71 33 22
AvaI C'yCGr_G
Cuts at: 0 114 206 272 279 1221
Size: 114 92 66 7 942
Fragments arranged by size:
942 114 92 66 7
BanI G'GyrC_C
Cuts at: 0 55 175 296 309 1221
Size: 55 120 121 13 912
Fragments arranged by size:
912 121 120 55 13
BanII G_rGCy'C
Cuts at: 0 234 1221
Size: 234 987
BbeI G_GCGC'C
Cuts at: 0 59 179 300 1221
Size: 59 120 121 921
Fragments arranged by size:
921 121 120 59
BfrBI ATG'CAT
Cuts at: 0 495 1221
Size: 495 726
Bme1580I G_kGCm'C
Cuts at: 0 234 314 1100 1221
Size: 234 80 786 121
Fragments arranged by size:
786 234 121 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 391 439 1221
Size: 143 248 48 782
Fragments arranged by size:
782 248 143 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1221
Size: 53 1168
BsaI GGTCTCn'nnnn_
Cuts at: 0 282 461 1221
Size: 282 179 760
Fragments arranged by size:
760 282 179
BsaHI Gr'CG_yC
Cuts at: 0 56 103 176 297 1221
Size: 56 47 73 121 924
Fragments arranged by size:
924 121 73 56 47
BsaWI w'CCGG_w
Cuts at: 0 124 1221
Size: 124 1097
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 1221
Size: 82 1139
BseYI C'CCAG_C
Cuts at: 0 227 1221
Size: 227 994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1221
Size: 159 1062
BsiHKAI G_wGCw'C
Cuts at: 0 1100 1221
Size: 1100 121
Bsp1286I G_dGCh'C
Cuts at: 0 234 314 1100 1221
Size: 234 80 786 121
Fragments arranged by size:
786 234 121 80
BspEI T'CCGG_A
Cuts at: 0 124 1221
Size: 124 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 1221
Size: 20 1201
BsrFI r'CCGG_y
Cuts at: 0 99 306 1221
Size: 99 207 915
Fragments arranged by size:
915 207 99
BsrGI T'GTAC_A
Cuts at: 0 577 1221
Size: 577 644
BstZ17I GTA'TAC
Cuts at: 0 368 1221
Size: 368 853
BtsI GCAGTG_nn'
Cuts at: 0 1094 1221
Size: 1094 127
DraI TTT'AAA
Cuts at: 0 459 1221
Size: 459 762
EaeI y'GGCC_r
Cuts at: 0 304 1221
Size: 304 917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1221
Size: 53 1168
EcoO109I rG'GnC_Cy
Cuts at: 0 231 857 1221
Size: 231 626 364
Fragments arranged by size:
626 364 231
HaeII r_GCGC'y
Cuts at: 0 59 179 241 300 324 635 1221
Size: 59 120 62 59 24 311 586
Fragments arranged by size:
586 311 120 62 59 59 24
KasI G'GCGC_C
Cuts at: 0 55 175 296 1221
Size: 55 120 121 925
Fragments arranged by size:
925 121 120 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 588 618 1221
Size: 403 185 30 603
Fragments arranged by size:
603 403 185 30
NarI GG'CG_CC
Cuts at: 0 56 176 297 1221
Size: 56 120 121 924
Fragments arranged by size:
924 121 120 56
NdeI CA'TA_TG
Cuts at: 0 1173 1221
Size: 1173 48
NsiI A_TGCA'T
Cuts at: 0 497 1221
Size: 497 724
NspI r_CATG'y
Cuts at: 0 90 720 1221
Size: 90 630 501
Fragments arranged by size:
630 501 90
PciI A'CATG_T
Cuts at: 0 716 1221
Size: 716 505
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 1221
Size: 31 1190
PfoI T'CCnGG_A
Cuts at: 0 66 1221
Size: 66 1155
PpuMI rG'GwC_Cy
Cuts at: 0 857 1221
Size: 857 364
PshAI GACnn'nnGTC
Cuts at: 0 332 1221
Size: 332 889
PsiI TTA'TAA
Cuts at: 0 1121 1221
Size: 1121 100
PspOMI G'GGCC_C
Cuts at: 0 230 1221
Size: 230 991
ScaI AGT'ACT
Cuts at: 0 132 1221
Size: 132 1089
SfoI GGC'GCC
Cuts at: 0 57 177 298 1221
Size: 57 120 121 923
Fragments arranged by size:
923 121 120 57
SgrAI Cr'CCGG_yG
Cuts at: 0 99 1221
Size: 99 1122
SmaI CCC'GGG
Cuts at: 0 274 1221
Size: 274 947
SmlI C'TyrA_G
Cuts at: 0 114 1221
Size: 114 1107
SphI G_CATG'C
Cuts at: 0 90 1221
Size: 90 1131
SspI AAT'ATT
Cuts at: 0 923 1132 1221
Size: 923 209 89
Fragments arranged by size:
923 209 89
StuI AGG'CCT
Cuts at: 0 112 1221
Size: 112 1109
SwaI ATTT'AAAT
Cuts at: 0 459 1221
Size: 459 762
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 125 729 1221
Size: 125 604 492
Fragments arranged by size:
604 492 125
TatI w'GTAC_w
Cuts at: 0 130 517 577 1221
Size: 130 387 60 644
Fragments arranged by size:
644 387 130 60
XhoI C'TCGA_G
Cuts at: 0 114 1221
Size: 114 1107
XmaI C'CCGG_G
Cuts at: 0 272 1221
Size: 272 949
XmnI GAAnn'nnTTC
Cuts at: 0 428 1221
Size: 428 793
Enzymes that do cut:
AccI AfeI AflIII ApaI ApaLI ApoI AvaI BanI
BanII BbeI BfrBI Bme1580I BmrI BpmI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BsrGI BstZ17I BtsI DraI EaeI Eco57MI EcoO109I
HaeII KasI MmeI NarI NdeI NsiI NspI PciI
PflMI PfoI PpuMI PshAI PsiI PspOMI ScaI SfoI
SgrAI SmaI SmlI SphI SspI StuI SwaI TaqII
TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SgfI SnaBI
SpeI SrfI StyI TstI Tth111I XbaI XcmI ZraI