################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI Asp718I AviII AvrII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BspDI BspEI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EagI EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HapII HindIII HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1146 GACGTC GACGT^C AcyI 1146 GACGTC G[GA]^CG[CT]C AflIII 723 ACATGT A^C[GA][CT]GT AhdI 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1451 AGCGCT AGC^GCT Asp700I 2413 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2081 GAGCAC G[AT]GC[AT]^C AspI 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2196 CTCGGG C^[CT]CG[GA]G BalI 2259 TGGCCA TGG^CCA BanI 1285 GGTGCC G^G[CT][GA]CC BcgI 1511 GCACAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2514 AGATCT A^GATCT BsaHI 1146 GACGTC G[GA]^CG[CT]C BsiEI 1168 CGATCG CG[GA][CT]^CG BsiHKAI 2081 GAGCAC G[AT]GC[AT]^C BsmI 2684 GCATTC GAATGC(1/-1) BsoBI 2196 CTCGGG C^[CT]CG[GA]G BspLU11I 723 ACATGT A^CATGT BssSI 2579 CACGAG CACGAG(-5/-1) BstBI 2369 TTCGAA TT^CGAA CfoI 1452 GCGC GCG^C DraI 2694 TTTAAA TTT^AAA DrdI 1731 GACTGTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2259 TGGCCA [CT]^GGCC[GA] Eam1105I 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1451 AGCGCT AGC^GCT EcoRI 742 GAATTC G^AATTC HaeII 1451 AGCGCT [GA]GCGC^[CT] HgaI 1747 GCGTC GACGC(5/10) HhaI 1452 GCGC GCG^C Hin1I 1146 GACGTC G[GA]^CG[CT]C HinP1I 1452 GCGC G^CGC HincII 2041 GTTAAC GT[CT]^[GA]AC HindII 2041 GTTAAC GT[CT]^[GA]AC HpaI 2041 GTTAAC GTT^AAC MluNI 2259 TGGCCA TGG^CCA MscI 2259 TGGCCA TGG^CCA NcoI 2343 CCATGG C^CATGG NdeI 1370 CATATG CA^TATG NspI 723 ACATGT [GA]CATG^[CT] NspV 2369 TTCGAA TT^CGAA PflFI 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1201 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1021 GGGTCCT [GA]G^G[AT]CC[CT] PvuI 1168 CGATCG CGAT^CG SfuI 2369 TTCGAA TT^CGAA Tth111I 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1201 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 45 TCTAGA T^CTAGA XcmI 1497 CCAGTGGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2413 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 324 GTCTAC GT^[AC][GT]AC 1969 GTCTAC AlwNI 1940 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2321 CAGCTCCTG AseI 2146 ATTAAT AT^TAAT 2305 ATTAAT AsnI 2146 ATTAAT AT^TAAT 2305 ATTAAT AvaII 442 GGACC G^G[AT]CC 1022 GGTCC BamHI 392 GGATCC G^GATCC 1596 GGATCC BbsI 255 GAAGAC GAAGAC(2/6) 1240 GAAGAC BpmI 332 CTCCAG CTGGAG(16/14) 1486 CTCCAG BpuAI 255 GAAGAC GAAGAC(2/6) 1240 GAAGAC BsaI 276 GAGACC GGTCTC(1/5) 289 GAGACC BstEII 168 GGTGACC G^GT[ACGT]ACC 958 GGTAACC BstPI 168 GGTGACC G^GT[ACGT]ACC 958 GGTAACC BstXI 1814 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2070 CCAGCAGAGTGG DsaI 1532 CCGTGG C^C[GA][CT]GG 2343 CCATGG EarI 2248 CTCTTC CTCTTC(1/4) 2417 CTCTTC EcoO65I 168 GGTGACC G^GT[ACGT]ACC 958 GGTAACC Ksp632I 2248 CTCTTC CTCTTC(1/4) 2417 CTCTTC MslI 855 CACTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1641 CACCAAAATG PshBI 2146 ATTAAT AT^TAAT 2305 ATTAAT SspI 1837 AATATT AAT^ATT 2656 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApaI ApoI BanII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII