(Circular) (Six-base) MAPSORT of: pFN21AE5004.seq Check: 9592 from: 1 to: 7447 pFN21AE5004 7447 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3935 3935 Size: 7447 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3155 5257 278 Size: 53 83 186 936 1619 2102 2468 Fragments arranged by size: 2468 2102 1619 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2330 3975 4790 1937 Size: 393 1645 815 4594 Fragments arranged by size: 4594 1645 815 393 Acc65I G'GTAC_C Cuts at: 4768 4768 Size: 7447 AclI AA'CG_TT Cuts at: 1563 5574 5947 1563 Size: 4011 373 3063 Fragments arranged by size: 4011 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3126 3366 4301 5510 6894 7162 1632 Size: 1494 240 935 1209 1384 268 1917 Fragments arranged by size: 1917 1494 1384 1209 935 268 240 AfeI AGC'GCT Cuts at: 3458 3458 Size: 7447 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7250 AflIII A'CryG_T Cuts at: 1203 2728 6346 7323 1203 Size: 1525 3618 977 1327 Fragments arranged by size: 3618 1525 1327 977 AgeI A'CCGG_T Cuts at: 6278 6278 Size: 7447 AhdI GACnn_n'nnGTC Cuts at: 4629 6176 4629 Size: 1547 5900 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7447 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7447 AlwNI CAG_nnn'CTG Cuts at: 3950 4331 5190 6762 3950 Size: 381 859 1572 4635 Fragments arranged by size: 4635 1572 859 381 ApaI G_GGCC'C Cuts at: 2062 3321 3427 2062 Size: 1259 106 6082 Fragments arranged by size: 6082 1259 106 ApaLI G'TGCA_C Cuts at: 5504 6660 5504 Size: 1156 6291 ApoI r'AATT_y Cuts at: 1493 2525 2747 3812 4290 4756 4982 5134 7243 Size: 1032 222 1065 478 466 226 152 2109 Cuts at: 7243 1493 Size: 1697 Fragments arranged by size: 2109 1697 1065 1032 478 466 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 4152 4311 6001 160 Size: 872 3120 159 1690 1606 Fragments arranged by size: 3120 1690 1606 872 159 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 AvaI C'yCGr_G Cuts at: 1870 1943 4201 4772 1870 Size: 73 2258 571 4545 Fragments arranged by size: 4545 2258 571 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2574 2607 2574 Size: 33 7414 BamHI G'GATC_C Cuts at: 2397 3601 4777 5144 2397 Size: 1204 1176 367 4700 Fragments arranged by size: 4700 1204 1176 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3290 4768 6223 618 Size: 325 217 627 1503 1478 1455 1842 Fragments arranged by size: 1842 1503 1478 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2482 3321 3427 4036 4766 1391 Size: 671 420 839 106 609 730 4072 Fragments arranged by size: 4072 839 730 671 609 420 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 3252 928 Size: 971 368 985 5123 Fragments arranged by size: 5123 985 971 368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3505 3539 5638 5672 1903 Size: 34 1568 34 2099 34 3678 Fragments arranged by size: 3678 2099 1568 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5339 6549 5339 Size: 1210 6237 BclI T'GATC_A Cuts at: 1548 1548 Size: 7447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6058 136 Size: 107 122 71 631 605 4386 1525 Fragments arranged by size: 4386 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4519 7442 4519 Size: 2923 4524 BlpI GC'TnA_GC Cuts at: 4864 4864 Size: 7447 Bme1580I G_kGCm'C Cuts at: 1790 2062 3321 3427 5508 6664 1790 Size: 272 1259 106 2081 1156 2573 Fragments arranged by size: 2573 2081 1259 1156 272 106 BmgBI CAC'GTC Cuts at: 1318 3744 1318 Size: 2426 5021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3600 5178 6136 Size: 416 53 83 399 24 2183 1578 958 Cuts at: 6136 442 Size: 1753 Fragments arranged by size: 2183 1753 1578 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7447 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 3476 6107 1239 Size: 1083 1154 2631 2579 Fragments arranged by size: 2631 2579 1154 1083 Bpu10I CC'TnA_GC Cuts at: 3681 3681 Size: 7447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2312 3315 3491 3964 5572 6437 6735 2312 Size: 1003 176 473 1608 865 298 3024 Fragments arranged by size: 3024 1608 1003 865 473 298 176 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2275 2288 6110 882 Size: 617 776 13 3822 2219 Fragments arranged by size: 3822 2219 776 617 13 BsaAI yAC'GTr Cuts at: 493 1204 5238 493 Size: 711 4034 2702 Fragments arranged by size: 4034 2702 711 BsaBI GATnn'nnATC Cuts at: 7441 7441 Size: 7447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3152 5254 5636 6271 Size: 53 83 186 936 1619 2102 382 635 Cuts at: 6271 275 Size: 1451 Fragments arranged by size: 2102 1619 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5880 6278 6552 6699 7110 5880 Size: 398 274 147 411 6217 Fragments arranged by size: 6217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3754 3784 4633 4663 1178 Size: 30 165 30 2351 30 849 30 3962 Fragments arranged by size: 3962 2351 849 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 3356 4056 1186 Size: 1139 1031 700 4577 Fragments arranged by size: 4577 1139 1031 700 BseYI C'CCAG_C Cuts at: 3022 3818 4074 6650 7146 3022 Size: 796 256 2576 496 3323 Fragments arranged by size: 3323 2576 796 496 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6874 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3176 4897 5658 6686 1540 Size: 275 186 1175 1721 761 1028 2301 Fragments arranged by size: 2301 1721 1175 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4090 5508 5593 6664 4090 Size: 1418 85 1071 4873 Fragments arranged by size: 4873 1418 1071 85 BsmI GAATG_Cn' Cuts at: 4689 4958 5051 4689 Size: 269 93 7085 Fragments arranged by size: 7085 269 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2482 3321 3427 4036 4090 4766 Size: 399 272 420 839 106 609 54 676 Cuts at: 4766 5508 5593 6664 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 839 742 676 609 420 399 272 106 85 54 BspEI T'CCGG_A Cuts at: 7110 7110 Size: 7447 BspHI T'CATG_A Cuts at: 5336 7066 5336 Size: 1730 5717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 3139 3935 4802 844 Size: 1717 578 796 867 3489 Fragments arranged by size: 3489 1717 867 796 578 BsrBI CCG'CTC Cuts at: 1394 1823 5334 1394 Size: 429 3511 3507 Fragments arranged by size: 3511 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2418 2897 3675 4527 4578 5942 6116 Size: 1183 1170 479 778 852 51 1364 174 Cuts at: 6116 65 Size: 1396 Fragments arranged by size: 1396 1364 1183 1170 852 778 479 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 6091 6278 1700 Size: 110 4281 187 2869 Fragments arranged by size: 4281 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2753 3067 4092 96 Size: 2657 314 1025 3451 Fragments arranged by size: 3451 2657 1025 314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7447 BssSI C'ACGA_G Cuts at: 4584 5507 6519 4584 Size: 923 1012 5512 Fragments arranged by size: 5512 1012 923 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7447 BstBI TT'CG_AA Cuts at: 4375 6251 4375 Size: 1876 5571 BstEII G'GTnAC_C Cuts at: 1184 2173 2963 1184 Size: 989 790 5668 Fragments arranged by size: 5668 989 790 BstXI CCAn_nnnn'nTGG Cuts at: 3826 4082 3826 Size: 256 7191 BstYI r'GATC_y Cuts at: 1972 2397 3215 3601 4519 4777 5144 5529 5546 Size: 425 818 386 918 258 367 385 17 Cuts at: 5546 6998 7084 7096 7442 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 918 818 425 386 385 367 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3537 4348 513 Size: 552 939 1533 811 3612 Fragments arranged by size: 3612 1533 939 811 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6708 Fragments arranged by size: 6708 683 56 BtsI GCAGTG_nn' Cuts at: 3106 4963 5757 5777 3106 Size: 1857 794 20 4776 Fragments arranged by size: 4776 1857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3268 3303 529 Size: 35 2704 35 4673 Fragments arranged by size: 4673 2704 35 35 DraI TTT'AAA Cuts at: 4701 4751 4701 Size: 50 7397 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3742 6454 809 Size: 507 2426 2712 1802 Fragments arranged by size: 2712 2426 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4264 4894 5783 Size: 54 1425 50 275 18 2434 630 889 Cuts at: 5783 7412 8 Size: 1629 43 Fragments arranged by size: 2434 1629 1425 889 630 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4894 1537 Size: 275 3082 4090 Fragments arranged by size: 4090 3082 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4259 4428 5377 1353 Size: 2906 169 949 3423 Fragments arranged by size: 3423 2906 949 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4301 4605 6034 6408 6554 387 Size: 1064 138 2712 304 1429 374 146 1280 Fragments arranged by size: 2712 1429 1280 1064 374 304 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2322 3126 3366 3476 4301 5510 6107 Size: 393 690 804 240 110 825 1209 597 Cuts at: 6107 6894 7162 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1209 825 804 787 690 597 393 268 240 110 EcoO109I rG'GnC_Cy Cuts at: 2058 2059 3027 3317 3318 3423 3424 2058 Size: 1 968 290 1 105 1 6081 Fragments arranged by size: 6081 968 290 105 1 1 1 EcoRI G'AATT_C Cuts at: 2747 4756 5134 2747 Size: 2009 378 5060 Fragments arranged by size: 5060 2009 378 FspI TGC'GCA Cuts at: 5953 5953 Size: 7447 HaeII r_GCGC'y Cuts at: 1678 3460 6594 1678 Size: 1782 3134 2531 Fragments arranged by size: 3134 2531 1782 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3011 3043 3332 3364 3474 Size: 32 135 32 1439 32 289 32 110 Cuts at: 3474 3506 4547 4579 4615 4628 4647 4660 6088 Size: 32 1041 32 36 13 19 13 1428 Cuts at: 6088 6120 6162 6194 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1439 1428 1041 289 135 110 42 36 32 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 4048 4791 5037 669 Size: 988 281 2110 743 246 3079 Fragments arranged by size: 3079 2110 988 743 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7447 HpaI GTT'AAC Cuts at: 4048 5037 4048 Size: 989 6458 KpnI G_GTAC'C Cuts at: 4772 4772 Size: 7447 MfeI C'AATT_G Cuts at: 5046 5046 Size: 7447 MluI A'CGCG_T Cuts at: 7323 7323 Size: 7447 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3230 4599 6561 6745 1305 Size: 1925 1369 1962 184 2007 Fragments arranged by size: 2007 1962 1925 1369 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4266 10 Size: 54 1425 343 2434 3191 Fragments arranged by size: 3191 2434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2864 3650 5405 5764 5923 518 Size: 648 1698 786 1755 359 159 2042 Fragments arranged by size: 2042 1755 1698 786 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2616 3165 4735 4863 5540 6688 6933 Size: 477 533 549 1570 128 677 1148 245 Cuts at: 6933 1606 Size: 2120 Fragments arranged by size: 2120 1570 1148 677 549 533 477 245 128 NcoI C'CATG_G Cuts at: 513 1065 2004 4348 513 Size: 552 939 2344 3612 Fragments arranged by size: 3612 2344 939 552 NdeI CA'TA_TG Cuts at: 387 3376 387 Size: 2989 4458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7447 NotI GC'GGCC_GC Cuts at: 4894 4894 Size: 7447 NspI r_CATG'y Cuts at: 1132 1229 2732 4805 6350 1132 Size: 97 1503 2073 1545 2229 Fragments arranged by size: 2229 2073 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7447 PciI A'CATG_T Cuts at: 2728 6346 2728 Size: 3618 3829 PflMI CCAn_nnn'nTGG Cuts at: 1108 3212 1108 Size: 2104 5343 PmeI GTTT'AAAC Cuts at: 4751 4751 Size: 7447 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5516 5548 7029 7061 5516 Size: 32 1481 32 5902 Fragments arranged by size: 5902 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3027 3027 Size: 7447 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7447 PsiI TTA'TAA Cuts at: 5017 5017 Size: 7447 PspOMI G'GGCC_C Cuts at: 2058 3317 3423 2058 Size: 1259 106 6082 Fragments arranged by size: 6082 1259 106 PstI C_TGCA'G Cuts at: 830 2907 3012 3136 3164 3328 4734 4799 830 Size: 2077 105 124 28 164 1406 65 3478 Fragments arranged by size: 3478 2077 1406 164 124 105 65 28 PvuI CG_AT'CG Cuts at: 2001 3176 2001 Size: 1175 6272 PvuII CAG'CTG Cuts at: 2083 2616 4735 2083 Size: 533 2119 4795 Fragments arranged by size: 4795 2119 533 SalI G'TCGA_C Cuts at: 1936 4789 1936 Size: 2853 4594 SbfI CC_TGCA'GG Cuts at: 4799 4799 Size: 7447 ScaI AGT'ACT Cuts at: 5695 7407 5695 Size: 1712 5735 SfcI C'TryA_G Cuts at: 826 1045 2205 2739 2903 3008 3132 3160 3324 Size: 219 1160 534 164 105 124 28 164 Cuts at: 3324 4730 4795 5930 6611 6802 826 Size: 1406 65 1135 681 191 1471 Fragments arranged by size: 1471 1406 1160 1135 681 534 219 191 164 164 124 105 65 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7447 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7447 SmaI CCC'GGG Cuts at: 1872 4774 1872 Size: 2902 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 3330 3470 3979 5551 6452 Size: 197 926 348 1039 140 509 1572 901 Cuts at: 6452 6714 820 Size: 262 1553 Fragments arranged by size: 1572 1553 1039 926 901 509 348 262 197 140 SnaBI TAC'GTA Cuts at: 493 5238 493 Size: 4745 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7447 SphI G_CATG'C Cuts at: 1132 4805 1132 Size: 3673 3774 SspI AAT'ATT Cuts at: 5 52 3844 4663 5371 5 Size: 47 3792 819 708 2081 Fragments arranged by size: 3792 2081 819 708 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7447 StyI C'CwwG_G Cuts at: 513 1065 2004 3850 3921 4348 4886 513 Size: 552 939 1846 71 427 538 3074 Fragments arranged by size: 3074 1846 939 552 538 427 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3252 5474 5491 5644 5829 6274 325 Size: 1255 1672 2222 17 153 185 445 1498 Fragments arranged by size: 2222 1672 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 2753 3067 Size: 274 80 33 51 1443 531 245 314 Cuts at: 3067 4092 4382 5693 7405 96 Size: 1025 290 1311 1712 138 Fragments arranged by size: 1712 1443 1311 1025 531 314 290 274 245 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7407 7439 7407 Size: 32 7415 Tth111I GACn'n_nGTC Cuts at: 1316 3971 1316 Size: 2655 4792 XbaI T'CTAG_A Cuts at: 2050 4783 2050 Size: 2733 4714 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3509 3509 Size: 7447 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7447 XmaI C'CCGG_G Cuts at: 1870 4772 1870 Size: 2902 4545 XmnI GAAnn'nnTTC Cuts at: 4422 5576 4422 Size: 1154 6293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3153 5255 276 Size: 53 83 186 936 1619 2102 2468 Fragments arranged by size: 2468 2102 1619 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI