(Linear) (Six-base) MAPSORT of: KIEE5004.seq Check: 9864 from: 1 to: 2742 KIEE5004.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1930 2742 Size: 1930 812 AatII G_ACGT'C Cuts at: 0 1150 2742 Size: 1150 1592 AccI GT'mk_AC Cuts at: 0 325 1970 2742 Size: 325 1645 772 Fragments arranged by size: 1645 772 325 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1121 1361 2296 2742 Size: 1121 240 935 446 Fragments arranged by size: 1121 935 446 240 AfeI AGC'GCT Cuts at: 0 1453 2742 Size: 1453 1289 AflIII A'CryG_T Cuts at: 0 723 2742 Size: 723 2019 AhdI GACnn_n'nnGTC Cuts at: 0 2624 2742 Size: 2624 118 AlwNI CAG_nnn'CTG Cuts at: 0 1945 2326 2742 Size: 1945 381 416 Fragments arranged by size: 1945 416 381 ApaI G_GGCC'C Cuts at: 0 57 1316 1422 2742 Size: 57 1259 106 1320 Fragments arranged by size: 1320 1259 106 57 ApoI r'AATT_y Cuts at: 0 520 742 1807 2285 2742 Size: 520 222 1065 478 457 Fragments arranged by size: 1065 520 478 457 222 AseI AT'TA_AT Cuts at: 0 2147 2306 2742 Size: 2147 159 436 Fragments arranged by size: 2147 436 159 AvaI C'yCGr_G Cuts at: 0 2196 2742 Size: 2196 546 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 569 602 2742 Size: 569 33 2140 Fragments arranged by size: 2140 569 33 BamHI G'GATC_C Cuts at: 0 392 1596 2742 Size: 392 1204 1146 Fragments arranged by size: 1204 1146 392 BanI G'GyrC_C Cuts at: 0 1285 2742 Size: 1285 1457 BanII G_rGCy'C Cuts at: 0 57 477 1316 1422 2031 2742 Size: 57 420 839 106 609 711 Fragments arranged by size: 839 711 609 420 106 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 262 1247 2742 Size: 262 985 1495 Fragments arranged by size: 1495 985 262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1500 1534 2742 Size: 1500 34 1208 Fragments arranged by size: 1500 1208 34 BglII A'GATC_T Cuts at: 0 2514 2742 Size: 2514 228 Bme1580I G_kGCm'C Cuts at: 0 57 1316 1422 2742 Size: 57 1259 106 1320 Fragments arranged by size: 1320 1259 106 57 BmgBI CAC'GTC Cuts at: 0 1739 2742 Size: 1739 1003 BmrI ACTGGGnnnn_n' Cuts at: 0 1595 2742 Size: 1595 1147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 317 1471 2742 Size: 317 1154 1271 Fragments arranged by size: 1271 1154 317 Bpu10I CC'TnA_GC Cuts at: 0 1676 2742 Size: 1676 1066 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1310 1486 1959 2742 Size: 307 1003 176 473 783 Fragments arranged by size: 1003 783 473 307 176 BsaI GGTCTCn'nnnn_ Cuts at: 0 270 283 2742 Size: 270 13 2459 Fragments arranged by size: 2459 270 13 BsaHI Gr'CG_yC Cuts at: 0 1147 2742 Size: 1147 1595 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1749 1779 2628 2658 2742 Size: 1749 30 849 30 84 Fragments arranged by size: 1749 849 84 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 320 1351 2051 2742 Size: 320 1031 700 691 Fragments arranged by size: 1031 700 691 320 BseYI C'CCAG_C Cuts at: 0 1017 1813 2069 2742 Size: 1017 796 256 673 Fragments arranged by size: 1017 796 673 256 BsiEI CG_ry'CG Cuts at: 0 1171 2742 Size: 1171 1571 BsiHKAI G_wGCw'C Cuts at: 0 2085 2742 Size: 2085 657 BsmI GAATG_Cn' Cuts at: 0 2684 2742 Size: 2684 58 Bsp1286I G_dGCh'C Cuts at: 0 57 477 1316 1422 2031 2085 2742 Size: 57 420 839 106 609 54 657 Fragments arranged by size: 839 657 609 420 106 57 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 556 1134 1930 2742 Size: 556 578 796 812 Fragments arranged by size: 812 796 578 556 BsrDI GCAATG_nn' Cuts at: 0 413 892 1670 2522 2573 2742 Size: 413 479 778 852 51 169 Fragments arranged by size: 852 778 479 413 169 51 BsrGI T'GTAC_A Cuts at: 0 748 1062 2087 2742 Size: 748 314 1025 655 Fragments arranged by size: 1025 748 655 314 BssSI C'ACGA_G Cuts at: 0 2579 2742 Size: 2579 163 BstBI TT'CG_AA Cuts at: 0 2370 2742 Size: 2370 372 BstEII G'GTnAC_C Cuts at: 0 168 958 2742 Size: 168 790 1784 Fragments arranged by size: 1784 790 168 BstXI CCAn_nnnn'nTGG Cuts at: 0 1821 2077 2742 Size: 1821 256 665 Fragments arranged by size: 1821 665 256 BstYI r'GATC_y Cuts at: 0 392 1210 1596 2514 2742 Size: 392 818 386 918 228 Fragments arranged by size: 918 818 392 386 228 BtgI C'CryG_G Cuts at: 0 1532 2343 2742 Size: 1532 811 399 Fragments arranged by size: 1532 811 399 BtsI GCAGTG_nn' Cuts at: 0 1101 2742 Size: 1101 1641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1263 1298 2742 Size: 1263 35 1444 Fragments arranged by size: 1444 1263 35 DraI TTT'AAA Cuts at: 0 2696 2742 Size: 2696 46 DrdI GACnn_nn'nnGTC Cuts at: 0 1737 2742 Size: 1737 1005 EaeI y'GGCC_r Cuts at: 0 2259 2742 Size: 2259 483 EarI CTCTTCn'nnn_ Cuts at: 0 2254 2423 2742 Size: 2254 169 319 Fragments arranged by size: 2254 319 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2296 2600 2742 Size: 2296 304 142 Fragments arranged by size: 2296 304 142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 317 1121 1361 1471 2296 2742 Size: 317 804 240 110 825 446 Fragments arranged by size: 825 804 446 317 240 110 EcoO109I rG'GnC_Cy Cuts at: 0 53 54 1022 1312 1313 1418 1419 2742 Size: 53 1 968 290 1 105 1 1323 Fragments arranged by size: 1323 968 290 105 53 1 1 1 EcoRI G'AATT_C Cuts at: 0 742 2742 Size: 742 2000 HaeII r_GCGC'y Cuts at: 0 1455 2742 Size: 1455 1287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1006 1038 1327 1359 1469 1501 2542 2574 Size: 1006 32 289 32 110 32 1041 32 Cuts at: 2574 2610 2623 2642 2655 2742 Size: 36 13 19 13 87 Fragments arranged by size: 1041 1006 289 110 87 36 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 0 2043 2742 Size: 2043 699 HpaI GTT'AAC Cuts at: 0 2043 2742 Size: 2043 699 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1225 2594 2742 Size: 1225 1369 148 Fragments arranged by size: 1369 1225 148 MscI TGG'CCA Cuts at: 0 2261 2742 Size: 2261 481 MslI CAynn'nnrTG Cuts at: 0 859 1645 2742 Size: 859 786 1097 Fragments arranged by size: 1097 859 786 MspA1I CmG'CkG Cuts at: 0 78 611 1160 2730 2742 Size: 78 533 549 1570 12 Fragments arranged by size: 1570 549 533 78 12 NcoI C'CATG_G Cuts at: 0 2343 2742 Size: 2343 399 NdeI CA'TA_TG Cuts at: 0 1371 2742 Size: 1371 1371 NspI r_CATG'y Cuts at: 0 727 2742 Size: 727 2015 PciI A'CATG_T Cuts at: 0 723 2742 Size: 723 2019 PflMI CCAn_nnn'nTGG Cuts at: 0 1207 2742 Size: 1207 1535 PpuMI rG'GwC_Cy Cuts at: 0 1022 2742 Size: 1022 1720 PspOMI G'GGCC_C Cuts at: 0 53 1312 1418 2742 Size: 53 1259 106 1324 Fragments arranged by size: 1324 1259 106 53 PstI C_TGCA'G Cuts at: 0 902 1007 1131 1159 1323 2729 2742 Size: 902 105 124 28 164 1406 13 Fragments arranged by size: 1406 902 164 124 105 28 13 PvuI CG_AT'CG Cuts at: 0 1171 2742 Size: 1171 1571 PvuII CAG'CTG Cuts at: 0 78 611 2730 2742 Size: 78 533 2119 12 Fragments arranged by size: 2119 533 78 12 SfcI C'TryA_G Cuts at: 0 200 734 898 1003 1127 1155 1319 2725 Size: 200 534 164 105 124 28 164 1406 Cuts at: 2725 2742 Size: 17 Fragments arranged by size: 1406 534 200 164 164 124 105 28 17 SmlI C'TyrA_G Cuts at: 0 286 1325 1465 1974 2742 Size: 286 1039 140 509 768 Fragments arranged by size: 1039 768 509 286 140 SspI AAT'ATT Cuts at: 0 1839 2658 2742 Size: 1839 819 84 Fragments arranged by size: 1839 819 84 StyI C'CwwG_G Cuts at: 0 1845 1916 2343 2742 Size: 1845 71 427 399 Fragments arranged by size: 1845 427 399 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1247 2742 Size: 1247 1495 TatI w'GTAC_w Cuts at: 0 503 748 1062 2087 2377 2742 Size: 503 245 314 1025 290 365 Fragments arranged by size: 1025 503 365 314 290 245 Tth111I GACn'n_nGTC Cuts at: 0 1966 2742 Size: 1966 776 XbaI T'CTAG_A Cuts at: 0 45 2742 Size: 45 2697 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1504 2742 Size: 1504 1238 XmnI GAAnn'nnTTC Cuts at: 0 2417 2742 Size: 2417 325 ZraI GAC'GTC Cuts at: 0 1148 2742 Size: 1148 1594 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AlwNI ApaI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BcgI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PpuMI PspOMI PstI PvuI PvuII SfcI SmlI SspI StyI TaqII TatI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AleI AlfI AloI ApaLI AscI AsiSI AvrII BbeI BbvCI BciVI BclI BfrBI BglI BlpI BmtI BplI BsaAI BsaBI BsaWI BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstZ17I Bsu36I BtgZI ClaI DraIII EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI XhoI XmaI