(Circular) (Six-base) MAPSORT of: pFN21AE0493.seq Check: 9626 from: 1 to: 5890 pFN21AE0493 5890 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2283 2283 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2696 3233 1937 Size: 759 537 4594 Fragments arranged by size: 4594 759 537 Acc65I G'GTAC_C Cuts at: 2586 3211 2586 Size: 625 5265 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 3953 5337 5605 1632 Size: 1046 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1275 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2555 1184 Size: 1371 4519 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 2430 Size: 32 5858 AlwNI CAG_nnn'CTG Cuts at: 2608 3633 5205 2608 Size: 1025 1572 3293 Fragments arranged by size: 3293 1572 1025 ApaI G_GGCC'C Cuts at: 2403 2403 Size: 5890 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 3104 3199 3425 3577 5686 1493 Size: 1611 95 226 152 2109 1697 Fragments arranged by size: 2109 1697 1611 226 152 95 AscI GG'CGCG_CC Cuts at: 2113 2113 Size: 5890 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 2201 2298 3215 1870 Size: 73 258 97 917 4545 Fragments arranged by size: 4545 917 258 97 73 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2160 2187 2586 3211 4666 Size: 325 217 627 373 27 399 625 1455 Cuts at: 4666 618 Size: 1842 Fragments arranged by size: 1842 1455 627 625 399 373 325 217 27 BanII G_rGCy'C Cuts at: 1391 2298 2403 2559 3209 1391 Size: 907 105 156 650 4072 Fragments arranged by size: 4072 907 650 156 105 BbeI G_GCGC'C Cuts at: 2191 2191 Size: 5890 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 2707 928 Size: 971 511 297 4111 Fragments arranged by size: 4111 971 511 297 BbvCI CC'TCA_GC Cuts at: 2210 2237 2210 Size: 27 5863 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2924 2958 4081 4115 1903 Size: 34 987 34 1123 34 3678 Fragments arranged by size: 3678 1123 987 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2563 4501 Size: 107 122 71 631 605 337 554 1938 Cuts at: 4501 136 Size: 1525 Fragments arranged by size: 1938 1525 631 605 554 337 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 3307 3307 Size: 5890 Bme1580I G_kGCm'C Cuts at: 1790 2090 2403 3951 5107 1790 Size: 300 313 1548 1156 2573 Fragments arranged by size: 2573 1548 1156 313 300 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3121 3621 4579 Size: 416 53 83 399 24 1704 500 958 Cuts at: 4579 442 Size: 1753 Fragments arranged by size: 1753 1704 958 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2998 2998 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 3065 4550 1239 Size: 1424 402 1485 2579 Fragments arranged by size: 2579 1485 1424 402 Bpu10I CC'TnA_GC Cuts at: 2210 2237 2210 Size: 27 5863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2454 2904 4015 4880 5178 2454 Size: 450 1111 865 298 3166 Fragments arranged by size: 3166 1111 865 450 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2969 4553 882 Size: 617 1470 1584 2219 Fragments arranged by size: 2219 1584 1470 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2188 3697 4079 Size: 53 83 186 936 603 52 1509 382 Cuts at: 4079 4714 275 Size: 635 1451 Fragments arranged by size: 1509 1451 936 635 603 382 186 83 53 52 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5665 Fragments arranged by size: 5665 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2306 3005 3172 1186 Size: 1033 87 699 167 3904 Fragments arranged by size: 3904 1033 699 167 87 BseYI C'CCAG_C Cuts at: 2279 2395 2403 5093 5589 2279 Size: 116 8 2690 496 2580 Fragments arranged by size: 2690 2580 496 116 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 1163 Size: 573 480 4837 Fragments arranged by size: 4837 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2393 3340 4101 5129 1540 Size: 275 186 12 380 947 761 1028 2301 Fragments arranged by size: 2301 1028 947 761 380 275 186 12 BsiHKAI G_wGCw'C Cuts at: 3951 4036 5107 3951 Size: 85 1071 4734 Fragments arranged by size: 4734 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2298 2403 2559 3209 3951 4036 Size: 399 300 208 105 156 650 742 85 Cuts at: 4036 5107 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 650 399 300 208 156 105 85 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2283 2616 2646 3245 844 Size: 1409 30 333 30 599 3489 Fragments arranged by size: 3489 1409 599 333 30 30 BsrBI CCG'CTC Cuts at: 1394 1823 2229 2389 2584 3777 1394 Size: 429 406 160 195 1193 3507 Fragments arranged by size: 3507 1193 429 406 195 160 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 4385 4559 65 Size: 1183 1254 1883 174 1396 Fragments arranged by size: 1883 1396 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 4534 4721 1700 Size: 110 620 2104 187 2869 Fragments arranged by size: 2869 2104 620 187 110 BsrGI T'GTAC_A Cuts at: 96 2649 96 Size: 2553 3337 BssHII G'CGCG_C Cuts at: 1925 2113 1925 Size: 188 5702 BssSI C'ACGA_G Cuts at: 2873 3950 4962 2873 Size: 1077 1012 3801 Fragments arranged by size: 3801 1077 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2504 794 Size: 1710 4180 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 5890 BstYI r'GATC_y Cuts at: 1972 2598 3220 3587 3972 3989 5441 5527 5539 Size: 626 622 367 385 17 1452 86 12 Cuts at: 5539 5885 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 626 622 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2678 2678 Size: 5890 BtgI C'CryG_G Cuts at: 513 1065 2004 2577 513 Size: 552 939 573 3826 Fragments arranged by size: 3826 939 573 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2688 487 Size: 683 56 1462 3689 Fragments arranged by size: 3689 1462 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2028 2058 Size: 54 1425 50 275 18 180 18 30 Cuts at: 2058 2456 3337 4226 5855 8 Size: 398 881 889 1629 43 Fragments arranged by size: 1629 1425 889 881 398 275 180 54 50 43 30 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3337 1537 Size: 275 198 1327 4090 Fragments arranged by size: 4090 1327 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2255 3820 1353 Size: 902 1565 3423 Fragments arranged by size: 3423 1565 902 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 2678 3065 3953 4550 5337 5605 Size: 393 1031 15 387 888 597 787 268 Cuts at: 5605 1239 Size: 1524 Fragments arranged by size: 1524 1031 888 787 597 393 387 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2957 2957 Size: 5890 EcoO109I rG'GnC_Cy Cuts at: 2181 2399 2400 2605 2181 Size: 218 1 205 5466 Fragments arranged by size: 5466 218 205 1 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 2146 2191 5037 1678 Size: 468 45 2846 2531 Fragments arranged by size: 2846 2531 468 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4531 4563 4605 4637 1373 Size: 32 135 32 2959 32 42 32 2626 Fragments arranged by size: 2959 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KasI G'GCGC_C Cuts at: 2187 2187 Size: 5890 KpnI G_GTAC'C Cuts at: 2590 3215 2590 Size: 625 5265 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5004 5188 1305 Size: 3699 184 2007 Fragments arranged by size: 3699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 10 Size: 54 1425 343 228 3840 Fragments arranged by size: 3840 1425 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2537 2789 2847 3848 4207 4366 Size: 648 1347 24 252 58 1001 359 159 Cuts at: 4366 518 Size: 2042 Fragments arranged by size: 2042 1347 1001 648 359 252 159 58 24 MspA1I CmG'CkG Cuts at: 1606 2024 2256 2407 3166 3306 3983 5131 5376 Size: 418 232 151 759 140 677 1148 245 Cuts at: 5376 1606 Size: 2120 Fragments arranged by size: 2120 1148 759 677 418 245 232 151 140 NaeI GCC'GGC Cuts at: 2432 2432 Size: 5890 NarI GG'CG_CC Cuts at: 2188 2188 Size: 5890 NcoI C'CATG_G Cuts at: 513 1065 2004 2577 513 Size: 552 939 573 3826 Fragments arranged by size: 3826 939 573 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NgoMIV G'CCGG_C Cuts at: 2430 2430 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 3248 4793 1132 Size: 97 2019 1545 2229 Fragments arranged by size: 2229 2019 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5890 PfoI T'CCnGG_A Cuts at: 2265 2601 2733 2265 Size: 336 132 5422 Fragments arranged by size: 5422 336 132 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2605 Size: 5890 PshAI GACnn'nnGTC Cuts at: 1613 3173 1613 Size: 1560 4330 PsiI TTA'TAA Cuts at: 2814 3460 2814 Size: 646 5244 PspOMI G'GGCC_C Cuts at: 2399 2399 Size: 5890 PstI C_TGCA'G Cuts at: 830 3242 830 Size: 2412 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2024 2407 3166 2024 Size: 383 759 4748 Fragments arranged by size: 4748 759 383 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SapI GCTCTTCn'nnn_ Cuts at: 2255 2255 Size: 5890 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 2171 4138 5850 2171 Size: 1967 1712 2211 Fragments arranged by size: 2211 1967 1712 SfcI C'TryA_G Cuts at: 826 1045 2818 3238 4373 5054 5245 826 Size: 219 1773 420 1135 681 191 1471 Fragments arranged by size: 1773 1471 1135 681 420 219 191 SfoI GGC'GCC Cuts at: 2189 2189 Size: 5890 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 2300 3217 1872 Size: 428 917 4545 Fragments arranged by size: 4545 917 428 SmlI C'TyrA_G Cuts at: 820 1017 1943 2469 2919 3994 4895 5157 820 Size: 197 926 526 450 1075 901 262 1553 Fragments arranged by size: 1553 1075 926 901 526 450 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 2235 1517 Size: 718 5172 StyI C'CwwG_G Cuts at: 513 1065 2004 2550 2577 3329 513 Size: 552 939 546 27 752 3074 Fragments arranged by size: 3074 939 752 552 546 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3917 3934 4087 4272 4717 325 Size: 1255 2337 17 153 185 445 1498 Fragments arranged by size: 2337 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 2442 2649 Size: 274 80 33 51 1443 192 273 207 Cuts at: 2649 2802 4136 5848 96 Size: 153 1334 1712 138 Fragments arranged by size: 1712 1443 1334 274 273 207 192 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3170 3202 5850 5882 3170 Size: 32 2648 32 3178 Fragments arranged by size: 3178 2648 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2567 2567 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 2298 3215 1870 Size: 428 917 4545 Fragments arranged by size: 4545 917 428 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI