Restriction map for
pFN21AB6825
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI MaeI MamI MfeI
MflI MluI MroI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 97 CGCG CG^CG
AflII 223 CTTAAG C^TTAAG
ApaI 47 GGGCCC GGGCC^C
AvaII 81 GGACC G^G[AT]CC
BalI 304 TGGCCA TGG^CCA
BanII 47 GGGCCC G[GA]GC[CT]^C
BcnI 84 CCCGG CC^[GC]GG
BfrI 223 CTTAAG C^TTAAG
BmyI 47 GGGCCC G[AGT]GC[ACT]^C
BpmI 52 CTGGAG CTGGAG(16/14)
BsaWI 103 TCCGGT [AT]^CCGG[AT]
BsgI 282 CTGCAC GTGCAG(16/14)
BsmAI 402 GAGAC GTCTC(1/5)
Bsp1286I 47 GGGCCC G[AGT]GC[ACT]^C
BspDI 339 ATCGAT AT^CGAT
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BstF5I 450 CATCC GGATG(2/0)
BstNI 51 CCTGG CC^[AT]GG
BstUI 97 CGCG CG^CG
ClaI 339 ATCGAT AT^CGAT
DpnII 338 GATC ^GATC
DraI 190 TTTAAA TTT^AAA
DraII 47 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 82 GACCCGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 96 CCGCGG C^C[GA][CT]GG
EaeI 304 TGGCCA [CT]^GGCC[GA]
EarI 28 GAAGAG CTCTTC(1/4)
EcoO109I 47 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 148 GAATTC G^AATTC
EcoRII 51 CCTGG ^CC[AT]GG
FauI 45 GCGGG CCCGC(4/6)
FokI 450 CATCC GGATG(9/13)
HincII 211 GTTAAC GT[CT]^[GA]AC
HindII 211 GTTAAC GT[CT]^[GA]AC
HinfI 142 GAATC G^A[ACGT]TC
HpaI 211 GTTAAC GTT^AAC
HphI 113 TCACC GGTGA(8/7)
Ksp632I 28 GAAGAG CTCTTC(1/4)
KspI 96 CCGCGG CCGC^GG
MboI 338 GATC ^GATC
MluNI 304 TGGCCA TGG^CCA
MscI 304 TGGCCA TGG^CCA
MvaI 51 CCTGG CC^[AT]GG
MvnI 97 CGCG CG^CG
NciI 84 CCCGG CC^[GC]GG
NdeII 338 GATC ^GATC
NlaIII 435 CATG CATG^
PvuII 61 CAGCTG CAG^CTG
SacII 96 CCGCGG CCGC^GG
Sau3AI 338 GATC ^GATC
ScaI 71 AGTACT AGT^ACT
SfaNI 200 GCATC GCATC(5/9)
TfiI 142 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 72 GTAC GT^AC
247 GTAC
BsaJI 50 CCCTGG C^C[ACGT][ACGT]GG
96 CCGCGG
BsrI 166 ACTGG ACTGG(1/-1)
307 CCAGT
BssKI 51 CCTGG ^CC[ACGT]GG
84 CCCGG
HaeIII 48 GGCC GG^CC
305 GGCC
MaeII 110 ACGT A^CGT
249 ACGT
MaeIII 107 GTCAC ^GT[ACGT]AC
112 GTCAC
MspA1I 61 CAGCTG C[AC]G^C[GT]G
96 CCGCGG
MwoI 269 GCCTTTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
387 GCTGTGCTTGC
RsaI 72 GTAC GT^AC
247 GTAC
ScrFI 51 CCTGG CC^[ACGT]GG
84 CCCGG
SmlI 67 CTCAAG C^T[CT][GA]AG
223 CTTAAG
Tsp45I 107 GTCAC ^GT[GC]AC
112 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BseRI Cac8I Cfr13I DdeI Fnu4HI
HapII HpaII ItaI MboII MnlI
MseI MspI NlaIV Sau96I TaqI
Tru9I TseI Tsp509I TthHB8I