Restriction map for
pFN21AB6129
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbrPI BciVI
BclI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 304 GTCTAC GT^[AC][GT]AC
AcsI 536 GAATTC [GA]^AATT[CT]
AflII 1591 CTTAAG C^TTAAG
AlwNI 874 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 223 CCTCAGG CC^T[ACGT]AGG
ApoI 536 GAATTC [GA]^AATT[CT]
AspHI 149 GAGCTC G[AT]GC[AT]^C
BamHI 1064 GGATCC G^GATCC
BfrI 1591 CTTAAG C^TTAAG
BlpI 451 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 149 GAGCTC G[AT]GC[AT]^C
BsmBI 1303 GAGACG CGTCTC(1/5)
BsrBI 510 CCGCTC CCGCTC(-3/-3)
BsrDI 1284 CATTGC GCAATG(2/0)
Bsu36I 223 CCTCAGG CC^T[ACGT]AGG
CelII 451 GCTGAGC GC^T[ACGT]AGC
CfoI 731 GCGC GCG^C
DraI 204 TTTAAA TTT^AAA
DraII 626 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 223 CCTCAGG CC^T[ACGT]AGG
EcoNI 399 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 626 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 536 GAATTC G^AATTC
EcoT14I 1088 CCAAGG C^C[AT][AT]GG
FauI 509 CCCGC CCCGC(4/6)
HaeII 730 AGCGCC [GA]GCGC^[CT]
HhaI 731 GCGC GCG^C
HinP1I 731 GCGC G^CGC
MspA1I 1114 CAGCTG C[AC]G^C[GT]G
PaeR7I 310 CTCGAG C^TCGAG
PpuMI 626 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1114 CAGCTG CAG^CTG
SacI 149 GAGCTC GAGCT^C
StyI 1088 CCAAGG C^C[AT][AT]GG
TaqI 311 TCGA T^CGA
TthHB8I 311 TCGA T^CGA
XbaI 846 TCTAGA T^CTAGA
XhoI 310 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 779 ACATGT A^C[GA][CT]GT
1716 ACATGT
Asp700I 156 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1249 GAATCTATTC
BcgI 696 CGAGACCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1278 CGATTTCATTGC
BpmI 168 CTCCAG CTGGAG(16/14)
1041 CTCCAG
BspHI 269 TCATGA T^CATGA
1122 TCATGA
BspLU11I 779 ACATGT A^CATGT
1716 ACATGT
BspMI 383 ACCTGC ACCTGC(4/8)
1154 ACCTGC
HindIII 862 AAGCTT A^AGCTT
1217 AAGCTT
PstI 1111 CTGCAG CTGCA^G
1725 CTGCAG
RcaI 269 TCATGA T^CATGA
1122 TCATGA
SfaNI 616 GATGC GCATC(5/9)
1609 GATGC
SmaI 515 CCCGGG CCC^GGG
809 CCCGGG
SmlI 310 CTCGAG C^T[CT][GA]AG
1591 CTTAAG
XmaCI 515 CCCGGG C^CCGGG
809 CCCGGG
XmaI 515 CCCGGG C^CCGGG
809 CCCGGG
XmnI 156 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1249 GAATCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII