(Circular) (Six-base) MAPSORT of: pFN21AB6129.seq Check: 2425 from: 1 to: 6466 pFN21AB6129 6466 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3168 3168 Size: 6466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4276 278 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2310 3809 1937 Size: 373 1499 4594 Fragments arranged by size: 4594 1499 373 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 3440 4529 5913 6181 1632 Size: 597 1211 1089 1384 268 1917 Fragments arranged by size: 1917 1384 1211 1089 597 268 AflII C'TTAA_G Cuts at: 820 1017 3596 820 Size: 197 2579 3690 Fragments arranged by size: 3690 2579 197 AflIII A'CryG_T Cuts at: 1203 2784 3721 5365 6342 1203 Size: 1581 937 1644 977 1327 Fragments arranged by size: 1644 1581 1327 977 937 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6466 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2930 2962 2930 Size: 32 6434 AlwNI CAG_nnn'CTG Cuts at: 2884 4209 5781 2884 Size: 1325 1572 3569 Fragments arranged by size: 3569 1572 1325 ApaLI G'TGCA_C Cuts at: 4523 5679 4523 Size: 1156 5310 ApoI r'AATT_y Cuts at: 1493 2541 3775 4001 4153 6262 1493 Size: 1048 1234 226 152 2109 1697 Fragments arranged by size: 2109 1697 1234 1048 226 152 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 2315 2520 2814 3791 1870 Size: 73 372 205 294 977 4545 Fragments arranged by size: 4545 977 372 294 205 73 BamHI G'GATC_C Cuts at: 3069 3796 4163 3069 Size: 727 367 5372 Fragments arranged by size: 5372 727 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3787 5242 618 Size: 325 217 627 2000 1455 1842 Fragments arranged by size: 2000 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2158 3549 3648 3661 3785 1391 Size: 767 1391 99 13 124 4072 Fragments arranged by size: 4072 1391 767 124 99 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 2262 2507 3404 928 Size: 971 336 27 245 897 3990 Fragments arranged by size: 3990 971 897 336 245 27 BbvCI CC'TCA_GC Cuts at: 2084 2221 3275 3694 2084 Size: 137 1054 419 4856 Fragments arranged by size: 4856 1054 419 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2690 2724 3272 3306 4657 Size: 34 50 34 669 34 548 34 1351 Cuts at: 4657 4691 1903 Size: 34 3678 Fragments arranged by size: 3678 1351 669 548 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 1548 Size: 6466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5077 136 Size: 107 122 71 631 605 3405 1525 Fragments arranged by size: 3405 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 2457 3883 2457 Size: 1426 5040 Bme1580I G_kGCm'C Cuts at: 1790 2101 4527 5683 1790 Size: 311 2426 1156 2573 Fragments arranged by size: 2573 2426 1156 311 BmgBI CAC'GTC Cuts at: 1318 2748 3325 1318 Size: 1430 577 4459 Fragments arranged by size: 4459 1430 577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2124 3520 4197 Size: 416 53 83 399 24 707 1396 677 Cuts at: 4197 5155 442 Size: 958 1753 Fragments arranged by size: 1753 1396 958 707 677 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 3031 5126 1239 Size: 919 873 2095 2579 Fragments arranged by size: 2579 2095 919 873 Bpu10I CC'TnA_GC Cuts at: 2084 2221 3275 3647 3694 2084 Size: 137 1054 372 47 4856 Fragments arranged by size: 4856 1054 372 137 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4591 5456 5754 4591 Size: 865 298 5303 Fragments arranged by size: 5303 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 2312 2696 3049 5129 882 Size: 617 547 266 384 353 2080 2219 Fragments arranged by size: 2219 2080 617 547 384 353 266 BsaAI yAC'GTr Cuts at: 493 1204 4257 493 Size: 711 3053 2702 Fragments arranged by size: 3053 2702 711 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4273 4655 5290 275 Size: 53 83 186 936 2740 382 635 1451 Fragments arranged by size: 2740 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4899 5297 5571 5718 6129 4899 Size: 398 274 147 411 5236 Fragments arranged by size: 5236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 2930 2960 1178 Size: 30 165 30 822 30 675 30 4684 Fragments arranged by size: 4684 822 675 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2217 2588 2842 1186 Size: 1031 371 254 4810 Fragments arranged by size: 4810 1031 371 254 BseYI C'CCAG_C Cuts at: 3660 5669 6165 3660 Size: 2009 496 3961 Fragments arranged by size: 3961 2009 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2113 2976 1163 Size: 573 318 59 863 4653 Fragments arranged by size: 4653 863 573 318 59 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3916 4677 5705 1540 Size: 275 186 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2158 4527 4612 5683 2158 Size: 2369 85 1071 2941 Fragments arranged by size: 2941 2369 1071 85 BsmI GAATG_Cn' Cuts at: 3977 4070 3977 Size: 93 6373 BsmBI CGTCTCn'nnnn_ Cuts at: 3302 3302 Size: 6466 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2158 3549 3648 3661 3785 4527 Size: 399 311 57 1391 99 13 124 742 Cuts at: 4527 4612 5683 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1391 1071 742 399 311 124 99 85 57 13 BspEI T'CCGG_A Cuts at: 6129 6129 Size: 6466 BspHI T'CATG_A Cuts at: 2274 3127 4355 6085 2274 Size: 853 1228 1730 2655 Fragments arranged by size: 2655 1730 1228 853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 3168 3821 844 Size: 1553 771 653 3489 Fragments arranged by size: 3489 1553 771 653 BsrBI CCG'CTC Cuts at: 1394 1823 2517 4353 1394 Size: 429 694 1836 3507 Fragments arranged by size: 3507 1836 694 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3288 4961 5135 65 Size: 1183 2040 1673 174 1396 Fragments arranged by size: 2040 1673 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5110 5297 1700 Size: 110 3300 187 2869 Fragments arranged by size: 3300 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 4526 5538 4526 Size: 1012 5454 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6466 BstBI TT'CG_AA Cuts at: 5270 5270 Size: 6466 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6466 BstYI r'GATC_y Cuts at: 1972 3055 3069 3153 3796 4163 4548 4565 6017 Size: 1083 14 84 643 367 385 17 1452 Cuts at: 6017 6103 6115 6461 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1083 643 385 367 346 86 84 17 14 12 Bsu36I CC'TnA_GG Cuts at: 2229 2229 Size: 6466 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5914 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5727 Fragments arranged by size: 5727 683 56 BtsI GCAGTG_nn' Cuts at: 2964 3982 4776 4796 2964 Size: 1018 794 20 4634 Fragments arranged by size: 4634 1018 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6431 DraI TTT'AAA Cuts at: 2211 3770 2211 Size: 1559 4907 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5473 809 Size: 507 4157 1802 Fragments arranged by size: 4157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3913 4802 6431 Size: 54 1425 50 275 18 2083 889 1629 Cuts at: 6431 8 Size: 43 Fragments arranged by size: 2083 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3913 1537 Size: 275 2101 4090 Fragments arranged by size: 4090 2101 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2372 3432 3757 4396 1353 Size: 1019 1060 325 639 3423 Fragments arranged by size: 3423 1060 1019 639 325 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5053 5427 5573 387 Size: 1064 138 3464 374 146 1280 Fragments arranged by size: 3464 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2156 2156 Size: 6466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2229 3031 3440 4529 5126 5913 Size: 393 526 71 802 409 1089 597 787 Cuts at: 5913 6181 1239 Size: 268 1524 Fragments arranged by size: 1524 1089 802 787 597 526 409 393 268 71 EcoNI CCTnn'n_nnAGG Cuts at: 2408 2408 Size: 6466 EcoO109I rG'GnC_Cy Cuts at: 2632 2632 Size: 6466 EcoRI G'AATT_C Cuts at: 2541 3775 4153 2541 Size: 1234 378 4854 Fragments arranged by size: 4854 1234 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2915 3032 3240 3622 2915 Size: 117 208 382 5759 Fragments arranged by size: 5759 382 208 117 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 2739 5613 1678 Size: 1061 2874 2531 Fragments arranged by size: 2874 2531 1061 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2225 2257 3029 Size: 32 135 32 584 32 37 32 772 Cuts at: 3029 3061 3126 3158 3711 3743 5107 5139 5181 Size: 32 65 32 553 32 1364 32 42 Cuts at: 5181 5213 1373 Size: 32 2626 Fragments arranged by size: 2626 1364 772 584 553 135 65 42 37 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3810 4056 669 Size: 988 281 1872 246 3079 Fragments arranged by size: 3079 1872 988 281 246 HindIII A'AGCT_T Cuts at: 748 2867 3222 748 Size: 2119 355 3992 Fragments arranged by size: 3992 2119 355 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3171 5580 5764 1305 Size: 1866 2409 184 2007 Fragments arranged by size: 2409 2007 1866 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4644 Fragments arranged by size: 4644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4424 4783 4942 518 Size: 648 3258 359 159 2042 Fragments arranged by size: 3258 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3121 3882 4559 5707 5952 1606 Size: 1515 761 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 761 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5914 NdeI CA'TA_TG Cuts at: 387 387 Size: 6466 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 2439 2788 3725 3824 5369 1132 Size: 97 1210 349 937 99 1545 2229 Fragments arranged by size: 2229 1545 1210 937 349 99 97 PasI CC'CwG_GG Cuts at: 1348 3641 1348 Size: 2293 4173 PciI A'CATG_T Cuts at: 2784 3721 5365 2784 Size: 937 1644 3885 Fragments arranged by size: 3885 1644 937 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6466 PfoI T'CCnGG_A Cuts at: 3345 3432 3345 Size: 87 6379 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 2962 4535 4567 6048 6080 2930 Size: 32 1573 32 1481 32 3316 Fragments arranged by size: 3316 1573 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 6466 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6466 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PstI C_TGCA'G Cuts at: 830 3120 3734 3818 830 Size: 2290 614 84 3478 Fragments arranged by size: 3478 2290 614 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 3121 3121 Size: 6466 SacI G_AGCT'C Cuts at: 2158 2158 Size: 6466 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SbfI CC_TGCA'GG Cuts at: 3818 3818 Size: 6466 ScaI AGT'ACT Cuts at: 4714 6426 4714 Size: 1712 4754 SfcI C'TryA_G Cuts at: 826 1045 3116 3382 3730 3814 4949 5630 5821 Size: 219 2071 266 348 84 1135 681 191 Cuts at: 5821 826 Size: 1471 Fragments arranged by size: 2071 1471 1135 681 348 266 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 2522 2816 3793 1872 Size: 650 294 977 4545 Fragments arranged by size: 4545 977 650 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 3596 4570 5471 5733 820 Size: 197 926 372 1281 974 901 262 1553 Fragments arranged by size: 1553 1281 974 926 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 4390 5 Size: 47 4338 2081 Fragments arranged by size: 4338 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6466 StyI C'CwwG_G Cuts at: 513 1065 3093 3905 513 Size: 552 2028 812 3074 Fragments arranged by size: 3074 2028 812 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2206 4493 4510 4663 4848 5293 325 Size: 1255 626 2287 17 153 185 445 1498 Fragments arranged by size: 2287 1498 1255 626 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3357 4712 6424 Size: 274 80 33 51 1443 1380 1355 1712 Cuts at: 6424 96 Size: 138 Fragments arranged by size: 1712 1443 1380 1355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6426 6458 6426 Size: 32 6434 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6466 XbaI T'CTAG_A Cuts at: 2851 3802 2851 Size: 951 5515 XhoI C'TCGA_G Cuts at: 1943 2315 1943 Size: 372 6094 XmaI C'CCGG_G Cuts at: 1870 2520 2814 3791 1870 Size: 650 294 977 4545 Fragments arranged by size: 4545 977 650 294 XmnI GAAnn'nnTTC Cuts at: 2165 3258 4595 2165 Size: 1093 1337 4036 Fragments arranged by size: 4036 1337 1093 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4274 276 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI