Restriction map for
pFN21AB6129
(Linear) (Six-base) MAPSORT of: KIBB6129.seq Check: 4603 from: 1 to: 1761
KIBB6129.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1163 1761
Size: 1163 598
AccI GT'mk_AC
Cuts at: 0 305 1761
Size: 305 1456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1435 1761
Size: 224 1211 326
Fragments arranged by size:
1211 326 224
AflII C'TTAA_G
Cuts at: 0 1591 1761
Size: 1591 170
AflIII A'CryG_T
Cuts at: 0 779 1716 1761
Size: 779 937 45
Fragments arranged by size:
937 779 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1761
Size: 925 32 804
Fragments arranged by size:
925 804 32
AlwNI CAG_nnn'CTG
Cuts at: 0 879 1761
Size: 879 882
ApoI r'AATT_y
Cuts at: 0 536 1761
Size: 536 1225
AvaI C'yCGr_G
Cuts at: 0 310 515 809 1761
Size: 310 205 294 952
Fragments arranged by size:
952 310 294 205
BamHI G'GATC_C
Cuts at: 0 1064 1761
Size: 1064 697
BanII G_rGCy'C
Cuts at: 0 153 1544 1643 1656 1761
Size: 153 1391 99 13 105
Fragments arranged by size:
1391 153 105 99 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 257 502 1399 1761
Size: 230 27 245 897 362
Fragments arranged by size:
897 362 245 230 27
BbvCI CC'TCA_GC
Cuts at: 0 79 216 1270 1689 1761
Size: 79 137 1054 419 72
Fragments arranged by size:
1054 419 137 79 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 719 1267 1301 1761
Size: 685 34 548 34 460
Fragments arranged by size:
685 548 460 34 34
BlpI GC'TnA_GC
Cuts at: 0 452 1761
Size: 452 1309
Bme1580I G_kGCm'C
Cuts at: 0 96 1761
Size: 96 1665
BmgBI CAC'GTC
Cuts at: 0 743 1320 1761
Size: 743 577 441
Fragments arranged by size:
743 577 441
BmrI ACTGGGnnnn_n'
Cuts at: 0 119 1515 1761
Size: 119 1396 246
Fragments arranged by size:
1396 246 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1026 1761
Size: 153 873 735
Fragments arranged by size:
873 735 153
Bpu10I CC'TnA_GC
Cuts at: 0 79 216 1270 1642 1689 1761
Size: 79 137 1054 372 47 72
Fragments arranged by size:
1054 372 137 79 72 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 41 307 691 1044 1761
Size: 41 266 384 353 717
Fragments arranged by size:
717 384 353 266 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 925 955 1761
Size: 220 30 675 30 806
Fragments arranged by size:
806 675 220 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 212 583 837 1761
Size: 212 371 254 924
Fragments arranged by size:
924 371 254 212
BseYI C'CCAG_C
Cuts at: 0 1655 1761
Size: 1655 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 108 971 1761
Size: 49 59 863 790
Fragments arranged by size:
863 790 59 49
BsiHKAI G_wGCw'C
Cuts at: 0 153 1761
Size: 153 1608
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1297 1761
Size: 1297 464
Bsp1286I G_dGCh'C
Cuts at: 0 96 153 1544 1643 1656 1761
Size: 96 57 1391 99 13 105
Fragments arranged by size:
1391 105 99 96 57 13
BspHI T'CATG_A
Cuts at: 0 269 1122 1761
Size: 269 853 639
Fragments arranged by size:
853 639 269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 1163 1761
Size: 392 771 598
Fragments arranged by size:
771 598 392
BsrBI CCG'CTC
Cuts at: 0 512 1761
Size: 512 1249
BsrDI GCAATG_nn'
Cuts at: 0 1283 1761
Size: 1283 478
BstYI r'GATC_y
Cuts at: 0 1050 1064 1148 1761
Size: 1050 14 84 613
Fragments arranged by size:
1050 613 84 14
Bsu36I CC'TnA_GG
Cuts at: 0 224 1761
Size: 224 1537
BtsI GCAGTG_nn'
Cuts at: 0 959 1761
Size: 959 802
DraI TTT'AAA
Cuts at: 0 206 1761
Size: 206 1555
EarI CTCTTCn'nnn_
Cuts at: 0 367 1427 1752 1761
Size: 367 1060 325 9
Fragments arranged by size:
1060 367 325 9
EcoICRI GAG'CTC
Cuts at: 0 151 1761
Size: 151 1610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 224 1026 1435 1761
Size: 153 71 802 409 326
Fragments arranged by size:
802 409 326 153 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 403 1761
Size: 403 1358
EcoO109I rG'GnC_Cy
Cuts at: 0 627 1761
Size: 627 1134
EcoRI G'AATT_C
Cuts at: 0 536 1761
Size: 536 1225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 910 1027 1235 1617 1761
Size: 910 117 208 382 144
Fragments arranged by size:
910 382 208 144 117
HaeII r_GCGC'y
Cuts at: 0 734 1761
Size: 734 1027
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 220 252 1024 1056 1121 1153
Size: 151 32 37 32 772 32 65 32
Cuts at: 1153 1706 1738 1761
Size: 553 32 23
Fragments arranged by size:
772 553 151 65 37 32 32 32
32 32 23
HindIII A'AGCT_T
Cuts at: 0 862 1217 1761
Size: 862 355 544
Fragments arranged by size:
862 544 355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1761
Size: 1166 595
MspA1I CmG'CkG
Cuts at: 0 1116 1761
Size: 1116 645
NspI r_CATG'y
Cuts at: 0 434 783 1720 1761
Size: 434 349 937 41
Fragments arranged by size:
937 434 349 41
PasI CC'CwG_GG
Cuts at: 0 1636 1761
Size: 1636 125
PciI A'CATG_T
Cuts at: 0 779 1716 1761
Size: 779 937 45
Fragments arranged by size:
937 779 45
PfoI T'CCnGG_A
Cuts at: 0 1340 1427 1761
Size: 1340 87 334
Fragments arranged by size:
1340 334 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1761
Size: 925 32 804
Fragments arranged by size:
925 804 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 1761
Size: 627 1134
PstI C_TGCA'G
Cuts at: 0 1115 1729 1761
Size: 1115 614 32
Fragments arranged by size:
1115 614 32
PvuII CAG'CTG
Cuts at: 0 1116 1761
Size: 1116 645
SacI G_AGCT'C
Cuts at: 0 153 1761
Size: 153 1608
SfcI C'TryA_G
Cuts at: 0 1111 1377 1725 1761
Size: 1111 266 348 36
Fragments arranged by size:
1111 348 266 36
SmaI CCC'GGG
Cuts at: 0 517 811 1761
Size: 517 294 950
Fragments arranged by size:
950 517 294
SmlI C'TyrA_G
Cuts at: 0 310 1591 1761
Size: 310 1281 170
Fragments arranged by size:
1281 310 170
StyI C'CwwG_G
Cuts at: 0 1088 1761
Size: 1088 673
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 201 1761
Size: 201 1560
TatI w'GTAC_w
Cuts at: 0 1352 1761
Size: 1352 409
XbaI T'CTAG_A
Cuts at: 0 846 1761
Size: 846 915
XhoI C'TCGA_G
Cuts at: 0 310 1761
Size: 310 1451
XmaI C'CCGG_G
Cuts at: 0 515 809 1761
Size: 515 294 952
Fragments arranged by size:
952 515 294
XmnI GAAnn'nnTTC
Cuts at: 0 160 1253 1761
Size: 160 1093 508
Fragments arranged by size:
1093 508 160
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AloI AlwNI ApoI
AvaI BamHI BanII BbsI BbvCI BcgI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BstYI Bsu36I BtsI DraI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HindIII MmeI MspA1I
NspI PasI PciI PfoI PpiI PpuMI PstI PvuII
SacI SfcI SmaI SmlI StyI TaqII TatI XbaI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BciVI BclI BfrBI BglI BglII BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XcmI
ZraI