Restriction map for
pFN21AB7777
(Circular) (Six-base) MAPSORT of: pFN21AB7777.seq Check: 7358 from: 1 to: 6064
pFN21AB7777 6064 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2950 3874 278
Size: 53 83 186 936 1414 924 2468
Fragments arranged by size:
2468 1414 936 924 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2560 3070 3407 1937
Size: 623 510 337 4594
Fragments arranged by size:
4594 623 510 337
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4127 5511 5779 1632
Size: 2495 1384 268 1917
Fragments arranged by size:
2495 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 3054 4963 5940 1203
Size: 1851 1909 977 1327
Fragments arranged by size:
1909 1851 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 3099 4793 3099
Size: 1694 4370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2243 3243 3807 5379 2243
Size: 1000 564 1572 2928
Fragments arranged by size:
2928 1572 1000 564
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2214 2876 3373 3599 3751 5860 1493
Size: 721 662 497 226 152 2109 1697
Fragments arranged by size:
2109 1697 721 662 497 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2610 3389 1870
Size: 73 667 779 4545
Fragments arranged by size:
4545 779 667 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3385 4840 618
Size: 325 217 627 1598 1455 1842
Fragments arranged by size:
1842 1598 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2417 2768 2918 3363 928
Size: 971 518 351 150 445 3629
Fragments arranged by size:
3629 971 518 445 351 150
BbvCI CC'TCA_GC
Cuts at: 2746 2746
Size: 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2058 2683 2723
Size: 416 53 83 399 24 641 625 40
Cuts at: 2723 3795 4753 442
Size: 1072 958 1753
Fragments arranged by size:
1753 1072 958 641 625 416 399 83
53 40 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3350 4724 1239
Size: 2111 1374 2579
Fragments arranged by size:
2579 2111 1374
Bpu10I CC'TnA_GC
Cuts at: 2746 2746
Size: 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3057 3855 493
Size: 711 1853 798 2702
Fragments arranged by size:
2702 1853 798 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2947 3871 4253 4888
Size: 53 83 186 936 1414 924 382 635
Cuts at: 4888 275
Size: 1451
Fragments arranged by size:
1451 1414 936 924 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 5109
BseYI C'CCAG_C
Cuts at: 5267 5763 5267
Size: 496 5568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 3514 4275 5303 1540
Size: 275 186 1261 252 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3383 4125 4210 5281 1391
Size: 399 1593 742 85 1071 2174
Fragments arranged by size:
2174 1593 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 3419 844
Size: 1547 1028 3489
Fragments arranged by size:
3489 1547 1028
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3185 4559 4733 65
Size: 1183 1937 1374 174 1396
Fragments arranged by size:
1937 1396 1374 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2458 2011
Size: 447 5617
BstYI r'GATC_y
Cuts at: 1972 2028 3225 3394 3761 4146 4163 5615 5701
Size: 56 1197 169 367 385 17 1452 86
Cuts at: 5701 5713 6059 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 169 86
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 513
Size: 552 939 102 4471
Fragments arranged by size:
4471 939 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 2969 3259
Size: 54 1425 50 275 18 630 509 290
Cuts at: 3259 3291 3511 4400 6029 8
Size: 32 220 889 1629 43
Fragments arranged by size:
1629 1425 889 630 509 290 275 220
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 3511 1537
Size: 275 1447 252 4090
Fragments arranged by size:
4090 1447 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2196 2352 2880 2985 3994 1353
Size: 717 126 156 528 105 1009 3423
Fragments arranged by size:
3423 1009 717 528 156 126 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 4651 5025 5171 387
Size: 1064 138 1125 1937 374 146 1280
Fragments arranged by size:
1937 1280 1125 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3350 4127 4724 5511 5779 1239
Size: 393 1718 777 597 787 268 1524
Fragments arranged by size:
1718 1524 787 777 597 393 268
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
EcoRV GAT'ATC
Cuts at: 2983 2983
Size: 6064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2699 2699
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 2573 2605 3015
Size: 32 135 32 647 32 322 32 410
Cuts at: 3015 3047 3085 3117 3164 3196 4705 4737 4779
Size: 32 38 32 47 32 1509 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 1509 647 410 322 135 47 42
38 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3071 3408 3654 669
Size: 988 281 1133 337 246 3079
Fragments arranged by size:
3079 1133 988 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 2916 3232 3663 2916
Size: 316 431 5317
Fragments arranged by size:
5317 431 316
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3083 3227 5178 5362 1305
Size: 1778 144 1951 184 2007
Fragments arranged by size:
2007 1951 1778 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 3293 10
Size: 54 1425 343 630 831 2781
Fragments arranged by size:
2781 1425 831 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4022 4381 4540 518
Size: 648 2856 359 159 2042
Fragments arranged by size:
2856 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3264 3480 4157 5305 5550 1606
Size: 1658 216 677 1148 245 2120
Fragments arranged by size:
2120 1658 1148 677 245 216
NcoI C'CATG_G
Cuts at: 513 1065 2004 2106 513
Size: 552 939 102 4471
Fragments arranged by size:
4471 939 552 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 3422 4967 1132
Size: 97 2193 1545 2229
Fragments arranged by size:
2229 2193 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2695 1108
Size: 1587 4477
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 3057 3057
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2085 2117 4133 4165 5646 5678 2085
Size: 32 2016 32 1481 32 2471
Fragments arranged by size:
2471 2016 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 3416 830
Size: 2586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3069 3406 1936
Size: 1133 337 4594
Fragments arranged by size:
4594 1133 337
SapI GCTCTTCn'nnn_
Cuts at: 2196 2196
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2557 3412 4547 5228 5419 826
Size: 219 1512 855 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 855 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 5069 5331 820
Size: 197 926 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 3503 513
Size: 552 939 102 1397 3074
Fragments arranged by size:
3074 1397 939 552 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3193 4091 4108 4261 4446 4891 325
Size: 1255 1613 898 17 153 185 445 1498
Fragments arranged by size:
1613 1498 1255 898 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 4310 6022
Size: 274 80 33 51 1443 960 1373 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1443 1373 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 6024 6056 3191
Size: 32 2801 32 3199
Fragments arranged by size:
3199 2801 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2946 1316
Size: 1630 4434
XbaI T'CTAG_A
Cuts at: 2405 2848 3400 2405
Size: 443 552 5069
Fragments arranged by size:
5069 552 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 3116 2455
Size: 661 5403
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2948 3872 276
Size: 53 83 186 936 1414 924 2468
Fragments arranged by size:
2468 1414 936 924 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI