(Circular) (Six-base) MAPSORT of: pFN21AE0305.seq Check: 2322 from: 1 to: 6661 pFN21AE0305 6661 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3680 3680 Size: 6661 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4471 278 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3556 4004 1937 Size: 1619 448 4594 Fragments arranged by size: 4594 1619 448 Acc65I G'GTAC_C Cuts at: 3003 3982 3003 Size: 979 5682 AclI AA'CG_TT Cuts at: 1563 4788 5161 1563 Size: 3225 373 3063 Fragments arranged by size: 3225 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 2357 4724 6108 6376 1632 Size: 697 28 2367 1384 268 1917 Fragments arranged by size: 2367 1917 1384 697 268 28 AfeI AGC'GCT Cuts at: 2140 2423 2140 Size: 283 6378 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 2982 5560 6537 1203 Size: 1779 2578 977 1327 Fragments arranged by size: 2578 1779 1327 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 2706 5390 2706 Size: 2684 3977 AleI CACnn'nnGTG Cuts at: 1166 3773 1166 Size: 2607 4054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6661 AlwNI CAG_nnn'CTG Cuts at: 2242 4404 5976 2242 Size: 2162 1572 2927 Fragments arranged by size: 2927 2162 1572 ApaI G_GGCC'C Cuts at: 2495 2591 2768 3305 3314 3444 3521 2495 Size: 96 177 537 9 130 77 5635 Fragments arranged by size: 5635 537 177 130 96 77 9 ApaLI G'TGCA_C Cuts at: 4718 5874 4718 Size: 1156 5505 ApoI r'AATT_y Cuts at: 1493 3970 4196 4348 6457 1493 Size: 2477 226 152 2109 1697 Fragments arranged by size: 2477 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2125 2761 3016 3304 3521 3986 Size: 73 114 68 636 255 288 217 465 Cuts at: 3986 1870 Size: 4545 Fragments arranged by size: 4545 636 465 288 255 217 114 73 68 AvrII C'CTAG_G Cuts at: 3763 3763 Size: 6661 BamHI G'GATC_C Cuts at: 3991 4358 3991 Size: 367 6294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3003 3527 3767 3982 5437 Size: 325 217 627 1216 524 240 215 1455 Cuts at: 5437 618 Size: 1842 Fragments arranged by size: 1842 1455 1216 627 524 325 240 217 215 BanII G_rGCy'C Cuts at: 1391 2295 2400 2495 2507 2591 2768 3305 3314 Size: 904 105 95 12 84 177 537 9 Cuts at: 3314 3444 3521 3645 3980 1391 Size: 130 77 124 335 4072 Fragments arranged by size: 4072 904 537 335 177 130 124 105 95 84 77 12 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5690 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2901 2935 4852 4886 1903 Size: 34 964 34 1917 34 3678 Fragments arranged by size: 3678 1917 964 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 2663 3309 Size: 107 122 71 631 605 592 399 646 Cuts at: 3309 3372 3525 5272 136 Size: 63 153 1747 1525 Fragments arranged by size: 1747 1525 646 631 605 592 399 153 122 107 71 63 BglII A'GATC_T Cuts at: 6656 6656 Size: 6661 BlpI GC'TnA_GC Cuts at: 2135 4078 2135 Size: 1943 4718 Bme1580I G_kGCm'C Cuts at: 1790 2495 2591 2652 2768 3070 3305 3314 3444 Size: 705 96 61 116 302 235 9 130 Cuts at: 3444 3489 3521 3530 4722 5878 1790 Size: 45 32 9 1192 1156 2573 Fragments arranged by size: 2573 1192 1156 705 302 235 130 116 96 61 45 32 9 9 BmgBI CAC'GTC Cuts at: 1318 2218 3040 3286 1318 Size: 900 822 246 4693 Fragments arranged by size: 4693 900 822 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2761 3609 4392 Size: 416 53 83 399 24 1344 848 783 Cuts at: 4392 5350 442 Size: 958 1753 Fragments arranged by size: 1753 1344 958 848 783 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2911 3159 3655 5321 1239 Size: 1040 632 248 496 1666 2579 Fragments arranged by size: 2579 1666 1040 632 496 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3518 3584 4786 5651 5949 3518 Size: 66 1202 865 298 4230 Fragments arranged by size: 4230 1202 865 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3403 5324 882 Size: 617 1904 1921 2219 Fragments arranged by size: 2219 1921 1904 617 BsaAI yAC'GTr Cuts at: 493 1204 2952 2985 4452 493 Size: 711 1748 33 1467 2702 Fragments arranged by size: 2702 1748 1467 711 33 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3576 3630 4468 4850 Size: 53 83 186 936 2043 54 838 382 Cuts at: 4850 5485 275 Size: 635 1451 Fragments arranged by size: 2043 1451 936 838 635 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 2400 5094 5492 5766 5913 6324 2400 Size: 2694 398 274 147 411 2737 Fragments arranged by size: 2737 2694 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3872 3902 3917 3947 1178 Size: 30 165 30 2469 30 15 30 3892 Fragments arranged by size: 3892 2469 165 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 3254 3467 3691 3860 1186 Size: 1033 1035 213 224 169 3987 Fragments arranged by size: 3987 1035 1033 224 213 169 BseYI C'CCAG_C Cuts at: 2052 2211 2235 2349 2451 2465 3111 3375 3644 Size: 159 24 114 102 14 646 264 269 Cuts at: 3644 5864 6360 2052 Size: 2220 496 2353 Fragments arranged by size: 2353 2220 646 496 269 264 159 114 102 24 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3187 1163 Size: 573 1451 4637 Fragments arranged by size: 4637 1451 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 3175 3482 4722 4807 5878 2400 Size: 775 307 1240 85 1071 3183 Fragments arranged by size: 3183 1240 1071 775 307 85 BsmI GAATG_Cn' Cuts at: 3053 3113 3877 4172 4265 3053 Size: 60 764 295 93 5449 Fragments arranged by size: 5449 764 295 93 60 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2295 2400 2495 2507 2591 2652 2768 Size: 399 505 105 95 12 84 61 116 Cuts at: 2768 3070 3175 3305 3314 3444 3482 3489 3521 Size: 302 105 130 9 130 38 7 32 Cuts at: 3521 3530 3645 3980 4722 4807 5878 1391 Size: 9 115 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 399 335 302 130 130 116 115 105 105 95 85 84 61 38 32 12 9 9 7 BspEI T'CCGG_A Cuts at: 2400 6324 2400 Size: 3924 2737 BspHI T'CATG_A Cuts at: 4550 6280 4550 Size: 1730 4931 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 4016 844 Size: 2836 336 3489 Fragments arranged by size: 3489 2836 336 BsrBI CCG'CTC Cuts at: 1394 1823 2458 4548 1394 Size: 429 635 2090 3507 Fragments arranged by size: 3507 2090 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5156 5330 65 Size: 1183 3908 174 1396 Fragments arranged by size: 3908 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2568 5305 5492 1700 Size: 110 758 2737 187 2869 Fragments arranged by size: 2869 2737 758 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6661 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 2407 4721 5733 2407 Size: 2314 1012 3335 Fragments arranged by size: 3335 2314 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3745 794 Size: 2951 3710 BstBI TT'CG_AA Cuts at: 5465 5465 Size: 6661 BstEII G'GTnAC_C Cuts at: 1184 2599 2896 1184 Size: 1415 297 4949 Fragments arranged by size: 4949 1415 297 BstXI CCAn_nnnn'nTGG Cuts at: 2017 3633 2017 Size: 1616 5045 BstYI r'GATC_y Cuts at: 1972 3991 4358 4743 4760 6212 6298 6310 6656 Size: 2019 367 385 17 1452 86 12 346 Cuts at: 6656 1972 Size: 1977 Fragments arranged by size: 2019 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2243 2243 Size: 6661 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2968 3408 3705 3771 3921 Size: 552 939 12 952 440 297 66 150 Cuts at: 3921 513 Size: 3253 Fragments arranged by size: 3253 952 939 552 440 297 150 66 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 2158 3226 4177 4971 4991 2158 Size: 1068 951 794 20 3828 Fragments arranged by size: 3828 1068 951 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 3965 3965 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2184 2664 Size: 54 1425 50 275 18 177 177 480 Cuts at: 2664 3255 3708 3777 4108 4997 6626 8 Size: 591 453 69 331 889 1629 43 Fragments arranged by size: 1629 1425 889 591 480 453 331 275 177 177 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 3833 4591 1353 Size: 836 1644 758 3423 Fragments arranged by size: 3423 1644 836 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5248 5622 5768 387 Size: 1064 138 3659 374 146 1280 Fragments arranged by size: 3659 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2398 2398 Size: 6661 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2329 2357 2911 3159 3655 4724 Size: 393 647 50 28 554 248 496 1069 Cuts at: 4724 5321 6108 6376 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1069 787 647 597 554 496 393 268 248 50 28 EcoNI CCTnn'n_nnAGG Cuts at: 2723 2723 Size: 6661 EcoO109I rG'GnC_Cy Cuts at: 2491 2492 2587 2734 2765 2963 3147 3310 3311 Size: 1 95 147 31 198 184 163 1 Cuts at: 3311 3348 3413 3441 3517 3518 3621 2491 Size: 37 65 28 76 1 103 5531 Fragments arranged by size: 5531 198 184 163 147 103 95 76 65 37 31 28 1 1 1 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2569 3576 2569 Size: 1007 5654 FspI TGC'GCA Cuts at: 5167 5167 Size: 6661 HaeII r_GCGC'y Cuts at: 1678 2142 2425 5808 1678 Size: 464 283 3383 2531 Fragments arranged by size: 3383 2531 464 283 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2692 2724 3872 3904 5302 Size: 32 135 32 1120 32 1148 32 1398 Cuts at: 5302 5334 5376 5408 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1398 1148 1120 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3082 4005 4251 669 Size: 988 281 1144 923 246 3079 Fragments arranged by size: 3079 1144 988 923 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3007 3986 3007 Size: 979 5682 MfeI C'AATT_G Cuts at: 4260 4260 Size: 6661 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3965 5775 5959 1305 Size: 2660 1810 184 2007 Fragments arranged by size: 2660 2007 1810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2186 3710 3779 10 Size: 54 1425 343 177 177 1524 69 2892 Fragments arranged by size: 2892 1524 1425 343 177 177 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 3773 4619 4978 5137 518 Size: 648 849 1758 846 359 159 2042 Fragments arranged by size: 2042 1758 849 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2335 2349 2991 3010 3025 3102 3665 4077 Size: 729 14 642 19 15 77 563 412 Cuts at: 4077 4754 5902 6147 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 729 677 642 563 412 245 77 19 15 14 NaeI GCC'GGC Cuts at: 2570 2570 Size: 6661 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 2968 3408 3921 513 Size: 552 939 12 952 440 513 3253 Fragments arranged by size: 3253 952 939 552 513 440 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6661 NgoMIV G'CCGG_C Cuts at: 2568 2568 Size: 6661 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2566 4019 5564 1132 Size: 97 1337 1453 1545 2229 Fragments arranged by size: 2229 1545 1453 1337 97 PasI CC'CwG_GG Cuts at: 1348 2581 3089 3196 1348 Size: 1233 508 107 4813 Fragments arranged by size: 4813 1233 508 107 PciI A'CATG_T Cuts at: 5560 5560 Size: 6661 PflMI CCAn_nnn'nTGG Cuts at: 1108 2939 3286 3437 1108 Size: 1831 347 151 4332 Fragments arranged by size: 4332 1831 347 151 PfoI T'CCnGG_A Cuts at: 3136 3136 Size: 6661 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PmlI CAC'GTG Cuts at: 2952 2985 2952 Size: 33 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2963 3348 3621 2963 Size: 385 273 6003 Fragments arranged by size: 6003 385 273 PshAI GACnn'nnGTC Cuts at: 1613 2325 3018 1613 Size: 712 693 5256 Fragments arranged by size: 5256 712 693 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PspOMI G'GGCC_C Cuts at: 2491 2587 2764 3301 3310 3440 3517 2491 Size: 96 177 537 9 130 77 5635 Fragments arranged by size: 5635 537 177 130 96 77 9 PspXI vC'TCGA_Gb Cuts at: 3016 3016 Size: 6661 PstI C_TGCA'G Cuts at: 830 3691 4013 830 Size: 2861 322 3478 Fragments arranged by size: 3478 2861 322 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 PvuII CAG'CTG Cuts at: 2349 3010 3025 3665 2349 Size: 661 15 640 5345 Fragments arranged by size: 5345 661 640 15 SacI G_AGCT'C Cuts at: 2400 2400 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SanDI GG'GwC_CC Cuts at: 2963 3621 2963 Size: 658 6003 SapI GCTCTTCn'nnn_ Cuts at: 2189 2189 Size: 6661 SbfI CC_TGCA'GG Cuts at: 3691 4013 3691 Size: 322 6339 ScaI AGT'ACT Cuts at: 2229 4909 6621 2229 Size: 2680 1712 2269 Fragments arranged by size: 2680 2269 1712 SexAI A'CCwGG_T Cuts at: 2892 2892 Size: 6661 SfcI C'TryA_G Cuts at: 826 1045 3687 4009 5144 5825 6016 826 Size: 219 2642 322 1135 681 191 1471 Fragments arranged by size: 2642 1471 1135 681 322 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3309 3309 Size: 6661 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 2059 3306 3988 1872 Size: 187 1247 682 4545 Fragments arranged by size: 4545 1247 682 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 3016 3533 3599 4765 5666 5928 Size: 197 926 1073 517 66 1166 901 262 Cuts at: 5928 820 Size: 1553 Fragments arranged by size: 1553 1166 1073 926 901 517 262 197 66 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 4019 1132 Size: 2887 3774 SspI AAT'ATT Cuts at: 5 52 4585 5 Size: 47 4533 2081 Fragments arranged by size: 4533 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6661 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2637 2968 2998 3408 3763 Size: 552 939 12 621 331 30 410 355 Cuts at: 3763 3921 4100 513 Size: 158 179 3074 Fragments arranged by size: 3074 939 621 552 410 355 331 179 158 30 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 3367 3559 4688 4705 4858 5043 Size: 1255 761 1026 192 1129 17 153 185 Cuts at: 5043 5488 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1129 1026 761 445 192 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2227 3933 4907 Size: 274 80 33 51 1443 250 1706 974 Cuts at: 4907 6619 96 Size: 1712 138 Fragments arranged by size: 1712 1706 1443 974 274 250 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6621 6653 6621 Size: 32 6629 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2635 2956 2635 Size: 321 6340 XhoI C'TCGA_G Cuts at: 1943 3016 1943 Size: 1073 5588 XmaI C'CCGG_G Cuts at: 1870 2057 3304 3986 1870 Size: 187 1247 682 4545 Fragments arranged by size: 4545 1247 682 187 XmnI GAAnn'nnTTC Cuts at: 2342 4790 2342 Size: 2448 4213 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4469 276 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SfoI SrfI SwaI