Restriction map for
pFN21AB6363
(Circular) (Six-base) MAPSORT of: pFN21AB6363.seq Check: 1314 from: 1 to: 5506
pFN21AB6363 5506 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 3569 4953 5221 1632
Size: 731 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 731 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 3249 4821 3249
Size: 1572 3934
ApaI G_GGCC'C
Cuts at: 2317 2317
Size: 5506
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2831 1870
Size: 73 888 4545
Fragments arranged by size:
4545 888 73
BamHI G'GATC_C
Cuts at: 2103 2836 3203 2103
Size: 733 367 4406
Fragments arranged by size:
4406 733 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2827 4282 618
Size: 325 217 627 444 596 1455 1842
Fragments arranged by size:
1842 1455 627 596 444 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2317 2376 2496 2733 2825 1391
Size: 621 305 59 120 237 92 4072
Fragments arranged by size:
4072 621 305 237 120 92 59
BbeI G_GCGC'C
Cuts at: 2235 2235
Size: 5506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2170 2787 928
Size: 971 271 617 3647
Fragments arranged by size:
3647 971 617 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2680 2714 3697 3731 1903
Size: 34 743 34 983 34 3678
Fragments arranged by size:
3678 983 743 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 136
Size: 107 122 71 631 605 2445 1525
Fragments arranged by size:
2445 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2011 2490 2923 2011
Size: 479 433 4594
Fragments arranged by size:
4594 479 433
Bme1580I G_kGCm'C
Cuts at: 1790 2317 3567 4723 1790
Size: 527 1250 1156 2573
Fragments arranged by size:
2573 1250 1156 527
BmgBI CAC'GTC
Cuts at: 1318 2770 1318
Size: 1452 4054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3237 4195 442
Size: 416 53 83 399 24 1820 958 1753
Fragments arranged by size:
1820 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 4166 1239
Size: 895 2032 2579
Fragments arranged by size:
2579 2032 895
Bpu10I CC'TnA_GC
Cuts at: 2159 2439 2159
Size: 280 5226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 3297 493
Size: 711 1227 866 2702
Fragments arranged by size:
2702 1227 866 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 3313 3695 4330
Size: 53 83 186 936 699 1081 382 635
Cuts at: 4330 275
Size: 1451
Fragments arranged by size:
1451 1081 936 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2137 1186
Size: 951 4555
BseYI C'CCAG_C
Cuts at: 2257 2457 4709 5205 2257
Size: 200 2252 496 2558
Fragments arranged by size:
2558 2252 496 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 2684 1163
Size: 573 717 231 3985
Fragments arranged by size:
3985 717 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2310 2956 3717 4745 1540
Size: 275 186 309 646 761 1028 2301
Fragments arranged by size:
2301 1028 761 646 309 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 2496 2733 3567 3652 4723 2376
Size: 120 237 834 85 1071 3159
Fragments arranged by size:
3159 1071 834 237 120 85
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2317 2376 2496 2733 2825 3567
Size: 399 222 305 59 120 237 92 742
Cuts at: 3567 3652 4723 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 237 222 120
92 85 59
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 2496 3395 5125 2496
Size: 899 1730 2877
Fragments arranged by size:
2877 1730 899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 844
Size: 2017 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 1394
Size: 429 1570 3507
Fragments arranged by size:
3507 1570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 4001 4175 65
Size: 1183 1044 1709 174 1396
Fragments arranged by size:
1709 1396 1183 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2763 4150 4337 1700
Size: 110 953 1387 187 2869
Fragments arranged by size:
2869 1387 953 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5506
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 2512 1184
Size: 1328 4178
BstYI r'GATC_y
Cuts at: 1972 2103 2836 3203 3588 3605 5057 5143 5155
Size: 131 733 367 385 17 1452 86 12
Cuts at: 5155 5501 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 733 385 367 346 131 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2508 2622 2508
Size: 114 5392
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4015
Fragments arranged by size:
4015 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 3022 3816 3836 2085
Size: 937 794 20 3755
Fragments arranged by size:
3755 937 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2307 2718
Size: 54 1425 50 275 18 279 198 411
Cuts at: 2718 2953 3842 5471 8
Size: 235 889 1629 43
Fragments arranged by size:
1629 1425 889 411 279 275 235 198
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2307 2953 1537
Size: 275 495 646 4090
Fragments arranged by size:
4090 646 495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2054 2324 2541 3436 1353
Size: 695 6 270 217 895 3423
Fragments arranged by size:
3423 895 695 270 217 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2374 2494 2731 2374
Size: 120 237 5149
Fragments arranged by size:
5149 237 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2363 3569 4166 4953 5221 1239
Size: 393 502 229 1206 597 787 268 1524
Fragments arranged by size:
1524 1206 787 597 502 393 268 229
EcoO109I rG'GnC_Cy
Cuts at: 2169 2313 2314 2587 2601 2794 2169
Size: 144 1 273 14 193 4881
Fragments arranged by size:
4881 273 193 144 14 1
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 2235 4653 1678
Size: 557 2418 2531
Fragments arranged by size:
2531 2418 557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373
Size: 32 135 32 2575 32 42 32 2626
Fragments arranged by size:
2626 2575 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2850 3096 669
Size: 988 281 301 611 246 3079
Fragments arranged by size:
3079 988 611 301 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2231 2231
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 4804 1305
Size: 3315 184 2007
Fragments arranged by size:
3315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 10
Size: 54 1425 343 888 2796
Fragments arranged by size:
2796 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 3464 3823 3982 518
Size: 648 1020 1278 359 159 2042
Fragments arranged by size:
2042 1278 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2250 2922 3599 4747 4992 1606
Size: 644 672 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 644 245
NaeI GCC'GGC
Cuts at: 2765 2765
Size: 5506
NarI GG'CG_CC
Cuts at: 2232 2232
Size: 5506
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4015
Fragments arranged by size:
4015 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NgoMIV G'CCGG_C
Cuts at: 2763 2763
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NruI TCG'CGA
Cuts at: 2400 2400
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2864 4409 1132
Size: 97 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2794 2601
Size: 193 5313
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspOMI G'GGCC_C
Cuts at: 2313 2313
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2415 2679 2858 830
Size: 1585 264 179 3478
Fragments arranged by size:
3478 1585 264 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SacI G_AGCT'C
Cuts at: 2376 2496 2733 2376
Size: 120 237 5149
Fragments arranged by size:
5149 237 120
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SbfI CC_TGCA'GG
Cuts at: 2415 2858 2415
Size: 443 5063
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2411 2675 2785 2854 3989 4670 4861
Size: 219 1366 264 110 69 1135 681 191
Cuts at: 4861 826
Size: 1471
Fragments arranged by size:
1471 1366 1135 681 264 219 191 110
69
SfoI GGC'GCC
Cuts at: 2233 2233
Size: 5506
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2833 1872
Size: 961 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5506
StyI C'CwwG_G
Cuts at: 513 1065 2004 2945 513
Size: 552 939 941 3074
Fragments arranged by size:
3074 941 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 3533 3550 3703 3888 4333 325
Size: 1255 1122 831 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 831 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 3752 5464
Size: 274 80 33 51 1443 288 1487 1712
Cuts at: 5464 96
Size: 138
Fragments arranged by size:
1712 1487 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 2759 2791 5466 5498 2593
Size: 32 134 32 2675 32 2601
Fragments arranged by size:
2675 2601 134 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2831 1870
Size: 961 4545
XmnI GAAnn'nnTTC
Cuts at: 2576 3635 2576
Size: 1059 4447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI