################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FseI HapII HgaI Hin1I HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1056 GTATAC GT^[AC][GT]AC AccII 80 CGCG CG^CG AflIII 701 ACGTGT A^C[GA][CT]GT AlwI 318 GGATC GGATC(4/5) ApaI 115 GGGCCC GGGCC^C AseI 874 ATTAAT AT^TAAT AsnI 874 ATTAAT AT^TAAT AspHI 644 GAGCAC G[AT]GC[AT]^C AvaI 41 CCCGAG C^[CT]CG[GA]G AviII 938 TGCGCA TGC^GCA BanII 115 GGGCCC G[GA]GC[CT]^C BclI 1124 TGATCA T^GATCA BfaI 347 CTAG C^TAG BglI 31 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 152 CTGGAG CTGGAG(16/14) BsaBI 570 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 644 GAGCAC G[AT]GC[AT]^C BsmAI 155 GAGAC GTCTC(1/5) BsoBI 41 CCCGAG C^[CT]CG[GA]G BspHI 566 TCATGA T^CATGA BssSI 775 CTCGTG CACGAG(-5/-1) Bst1107I 1056 GTATAC GTA^TAC BstAPI 90 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 80 CGCG CG^CG BstYI 318 GGATCT [GA]^GATC[CT] BstZ17I 1056 GTATAC GTA^TAC FauI 81 GCGGG CCCGC(4/6) FbaI 1124 TGATCA T^GATCA FspI 938 TGCGCA TGC^GCA HincII 64 GTCAAC GT[CT]^[GA]AC HindII 64 GTCAAC GT[CT]^[GA]AC HindIII 612 AAGCTT A^AGCTT MaeI 347 CTAG C^TAG MamI 570 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 318 GGATCT [GA]^GATC[CT] MvnI 80 CGCG CG^CG PflMI 1225 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1012 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 874 ATTAAT AT^TAAT PstI 88 CTGCAG CTGCA^G RcaI 566 TCATGA T^CATGA SfiI 30 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1182 CTTGAG C^T[CT][GA]AG SphI 682 GCATGC GCATG^C StuI 336 AGGCCT AGG^CCT Tsp45I 1114 GTCAC ^GT[GC]AC Van91I 1225 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 318 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 272 GTAC GT^AC 470 GTAC AlwNI 506 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1226 CAGGTGCTG Asp700I 334 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1574 GAATGTCTTC BalI 452 TGGCCA TGG^CCA 530 TGGCCA BanI 124 GGCACC G^G[CT][GA]CC 1234 GGTGCC BbsI 12 GAAGAC GAAGAC(2/6) 1578 GTCTTC BpuAI 12 GAAGAC GAAGAC(2/6) 1578 GTCTTC BsgI 1521 CTGCAC GTGCAG(16/14) 755 GTGCAG BsmFI 1032 GGGAC GGGAC(10/14) 1175 GGGAC BsrI 262 ACTGG ACTGG(1/-1) 1280 CCAGT DraII 114 GGGGCCC [GA]G^G[ACGT]CC[CT] 386 AGGCCCT DsaI 639 CCACGG C^C[GA][CT]GG 1238 CCGTGG EaeI 452 TGGCCA [CT]^GGCC[GA] 530 TGGCCA EcoO109I 114 GGGGCCC [GA]G^G[ACGT]CC[CT] 386 AGGCCCT EcoRI 732 GAATTC G^AATTC 999 GAATTC HaeII 54 GGCGCT [GA]GCGC^[CT] 148 GGCGCT MaeII 95 ACGT A^CGT 701 ACGT MluNI 452 TGGCCA TGG^CCA 530 TGGCCA MscI 452 TGGCCA TGG^CCA 530 TGGCCA MslI 129 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 579 CATGGAGATG NspI 682 GCATGC [GA]CATG^[CT] 1035 ACATGC RsaI 272 GTAC GT^AC 470 GTAC SfcI 88 CTGCAG C^T[GA][CT]AG 512 CTGTAG TfiI 850 GATTC G^A[AT]TC 1414 GAATC XcmI 26 CCAAGGCCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1538 CCAACATTCTCTTGG XmnI 334 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1574 GAATGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrDI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NsiI PleI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp509I TspRI