Restriction map for
pFN21AB5054
(Circular) (Six-base) MAPSORT of: pFN21AB5054.seq Check: 8464 from: 1 to: 6298
pFN21AB5054 6298 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 3641 1937
Size: 1125 579 4594
Fragments arranged by size:
4594 1125 579
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2358 2381 2385 3365 4361 5745 6013 1632
Size: 726 23 4 980 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 980 726 268 23 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 2706 5197 6174 1203
Size: 1503 2491 977 1327
Fragments arranged by size:
2491 1503 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 1184
Size: 1023 5275
AlwNI CAG_nnn'CTG
Cuts at: 2516 3236 4041 5613 2516
Size: 720 805 1572 3201
Fragments arranged by size:
3201 1572 805 720
ApaI G_GGCC'C
Cuts at: 2124 2124
Size: 6298
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 2208 2737 2798 3004 3399 3607 3833 3985
Size: 715 529 61 206 395 208 226 152
Cuts at: 3985 6094 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 715 529 395 226 208 206
152 61
AseI AT'TA_AT
Cuts at: 160 1032 2880 4852 160
Size: 872 1848 1972 1606
Fragments arranged by size:
1972 1848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 3623 1870
Size: 73 103 1577 4545
Fragments arranged by size:
4545 1577 103 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 3239 3619 5074 618
Size: 325 217 627 342 1110 380 1455 1842
Fragments arranged by size:
1842 1455 1110 627 380 342 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 3617 1391
Size: 733 1493 4072
Fragments arranged by size:
4072 1493 733
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 3576 928
Size: 971 125 1552 3650
Fragments arranged by size:
3650 1552 971 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 3129 1548
Size: 1581 4717
BfrBI ATG'CAT
Cuts at: 2687 2691 3332 2687
Size: 4 641 5653
Fragments arranged by size:
5653 641 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2042 4909
Size: 107 122 71 631 605 337 33 2867
Cuts at: 4909 136
Size: 1525
Fragments arranged by size:
2867 1525 631 605 337 122 107 71
33
BglII A'GATC_T
Cuts at: 6293 6293
Size: 6298
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2132 3304 4359 5515 1790
Size: 334 8 1172 1055 1156 2573
Fragments arranged by size:
2573 1172 1156 1055 334 8
BmgBI CAC'GTC
Cuts at: 1318 2101 1318
Size: 783 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4029 4987
Size: 416 53 83 399 24 1862 750 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
1862 1753 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2994 2795
Size: 199 6099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 4958 1239
Size: 939 2780 2579
Fragments arranged by size:
2780 2579 939
Bpu10I CC'TnA_GC
Cuts at: 2105 2105
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3208 4423 5288 5586 3208
Size: 1215 865 298 3920
Fragments arranged by size:
3920 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4961 882
Size: 617 3462 2219
Fragments arranged by size:
3462 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 2579 6292 2579
Size: 3713 2585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4105 4487 5122 275
Size: 53 83 186 936 2572 382 635 1451
Fragments arranged by size:
2572 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4731 5129 5403 5550 5961 4731
Size: 398 274 147 411 5068
Fragments arranged by size:
5068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6073
Fragments arranged by size:
6073 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6298
BseYI C'CCAG_C
Cuts at: 5501 5997 5501
Size: 496 5802
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2781 3511 1163
Size: 573 1045 730 3950
Fragments arranged by size:
3950 1045 730 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2653 4359 4444 5515 2653
Size: 1706 85 1071 3436
Fragments arranged by size:
3436 1706 1071 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2132 2653 3304 3617 4359 4444
Size: 399 334 8 521 651 313 742 85
Cuts at: 4444 5515 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 651 521 399 334 313
85 8
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 2571 4187 5917 2571
Size: 1616 1730 2952
Fragments arranged by size:
2952 1730 1616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3653 844
Size: 2809 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 3261 3332 4793 4967 65
Size: 1183 943 1070 71 1461 174 1396
Fragments arranged by size:
1461 1396 1183 1070 943 174 71
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 2780 4358 5370 2780
Size: 1578 1012 3708
Fragments arranged by size:
3708 1578 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 794
Size: 1307 4991
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2323 3628 3995 4380 4397 5849 5935 5947
Size: 351 1305 367 385 17 1452 86 12
Cuts at: 5947 6293 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1305 385 367 351 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3063 3063
Size: 6298
BtgI C'CryG_G
Cuts at: 513 1065 2004 2644 3243 513
Size: 552 939 640 599 3568
Fragments arranged by size:
3568 939 640 599 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 3180 3814 4608 4628 3180
Size: 634 794 20 4850
Fragments arranged by size:
4850 794 634 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 2535 3745
Size: 54 1425 50 275 18 627 78 1210
Cuts at: 3745 4634 6263 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1210 889 627 275 78 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 2573 2760 3069 4228 1353
Size: 1122 98 187 309 1159 3423
Fragments arranged by size:
3423 1159 1122 309 187 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 4885 5259 5405 387
Size: 1064 138 747 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 747 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2358 2381 2385 3365 4361 4958
Size: 393 546 180 23 4 980 996 597
Cuts at: 4958 5745 6013 1239
Size: 787 268 1524
Fragments arranged by size:
1524 996 980 787 597 546 393 268
180 23 4
EcoO109I rG'GnC_Cy
Cuts at: 2120 2392 2120
Size: 272 6026
EcoRI G'AATT_C
Cuts at: 2737 3004 3607 3985 2737
Size: 267 603 378 5050
Fragments arranged by size:
5050 603 378 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 6298
FspI TGC'GCA
Cuts at: 2945 4804 2945
Size: 1859 4439
HaeII r_GCGC'y
Cuts at: 1678 2063 2157 5445 1678
Size: 385 94 3288 2531
Fragments arranged by size:
3288 2531 385 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4939 4971 5013 5045 1373
Size: 32 135 32 3367 32 42 32 2626
Fragments arranged by size:
3367 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2071 3642 3888 669
Size: 988 281 133 1571 246 3079
Fragments arranged by size:
3079 1571 988 281 246 133
HindIII A'AGCT_T
Cuts at: 748 2617 748
Size: 1869 4429
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5412 5596 1305
Size: 4107 184 2007
Fragments arranged by size:
4107 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2537 10
Size: 54 1425 343 627 78 3771
Fragments arranged by size:
3771 1425 627 343 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2588 4256 4615 4774 518
Size: 648 972 450 1668 359 159 2042
Fragments arranged by size:
2042 1668 972 648 450 359 159
MspA1I CmG'CkG
Cuts at: 1606 3714 4391 5539 5784 1606
Size: 2108 677 1148 245 2120
Fragments arranged by size:
2120 2108 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4807
Fragments arranged by size:
4807 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NsiI A_TGCA'T
Cuts at: 2689 2693 3334 2689
Size: 4 641 5653
Fragments arranged by size:
5653 641 4
NspI r_CATG'y
Cuts at: 1132 1229 2691 3044 3656 5201 1132
Size: 97 1462 353 612 1545 2229
Fragments arranged by size:
2229 1545 1462 612 353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3236 1108
Size: 2128 4170
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3021 1613
Size: 1408 4890
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PspOMI G'GGCC_C
Cuts at: 2120 2120
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2097 3650 830
Size: 1267 1553 3478
Fragments arranged by size:
3478 1553 1267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SfcI C'TryA_G
Cuts at: 826 1045 2093 2517 3646 4781 5462 5653 826
Size: 219 1048 424 1129 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 1048 681 424 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2042 2042
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3187 4402 5303 5565 820
Size: 197 926 1244 1215 901 262 1553
Fragments arranged by size:
1553 1244 1215 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 2691 3656 1132
Size: 1559 965 3774
Fragments arranged by size:
3774 1559 965
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 2343 1517
Size: 826 5472
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2124 3348 3737 513
Size: 552 939 27 93 1224 389 3074
Fragments arranged by size:
3074 1224 939 552 389 93 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2232 3425 4325 4342 4495 4680 5125
Size: 1255 652 1193 900 17 153 185 445
Cuts at: 5125 325
Size: 1498
Fragments arranged by size:
1498 1255 1193 900 652 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4544 6256 96
Size: 274 80 33 51 1443 2567 1712 138
Fragments arranged by size:
2567 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2833 2865 6258 6290 2833
Size: 32 3393 32 2841
Fragments arranged by size:
3393 2841 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 3550 2038
Size: 1512 4786
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 2343 3583 4427 2343
Size: 1240 844 4214
Fragments arranged by size:
4214 1240 844
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI