(Linear) (Six-base) MAPSORT of: KIBB5054.seq Check: 6255 from: 1 to: 1593 KIBB5054.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AccI GT'mk_AC Cuts at: 0 1057 1593 Size: 1057 536 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 353 376 380 1360 1593 Size: 353 23 4 980 233 Fragments arranged by size: 980 353 233 23 4 AflIII A'CryG_T Cuts at: 0 701 1593 Size: 701 892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 202 1593 Size: 202 1391 AlwNI CAG_nnn'CTG Cuts at: 0 511 1231 1593 Size: 511 720 362 Fragments arranged by size: 720 511 362 ApaI G_GGCC'C Cuts at: 0 119 1593 Size: 119 1474 ApoI r'AATT_y Cuts at: 0 203 732 793 999 1394 1593 Size: 203 529 61 206 395 199 Fragments arranged by size: 529 395 206 203 199 61 AseI AT'TA_AT Cuts at: 0 875 1593 Size: 875 718 AvaI C'yCGr_G Cuts at: 0 41 1593 Size: 41 1552 BanI G'GyrC_C Cuts at: 0 124 1234 1593 Size: 124 1110 359 Fragments arranged by size: 1110 359 124 BanII G_rGCy'C Cuts at: 0 119 1593 Size: 119 1474 BbsI GAAGACnn'nnnn_ Cuts at: 0 19 1571 1593 Size: 19 1552 22 Fragments arranged by size: 1552 22 19 BclI T'GATC_A Cuts at: 0 1124 1593 Size: 1124 469 BfrBI ATG'CAT Cuts at: 0 682 686 1327 1593 Size: 682 4 641 266 Fragments arranged by size: 682 641 266 4 BglI GCCn_nnn'nGGC Cuts at: 0 37 1593 Size: 37 1556 Bme1580I G_kGCm'C Cuts at: 0 119 127 1299 1593 Size: 119 8 1172 294 Fragments arranged by size: 1172 294 119 8 BmgBI CAC'GTC Cuts at: 0 96 1593 Size: 96 1497 BmrI ACTGGGnnnn_n' Cuts at: 0 1274 1593 Size: 1274 319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 790 989 1593 Size: 790 199 604 Fragments arranged by size: 790 604 199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 1593 Size: 173 1420 Bpu10I CC'TnA_GC Cuts at: 0 100 1593 Size: 100 1493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1203 1593 Size: 1203 390 BsaBI GATnn'nnATC Cuts at: 0 574 1593 Size: 574 1019 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 776 1506 1593 Size: 776 730 87 Fragments arranged by size: 776 730 87 BsiHKAI G_wGCw'C Cuts at: 0 648 1593 Size: 648 945 Bsp1286I G_dGCh'C Cuts at: 0 119 127 648 1299 1593 Size: 119 8 521 651 294 Fragments arranged by size: 651 521 294 119 8 BspHI T'CATG_A Cuts at: 0 566 1593 Size: 566 1027 BsrDI GCAATG_nn' Cuts at: 0 186 1256 1327 1593 Size: 186 1070 71 266 Fragments arranged by size: 1070 266 186 71 BssSI C'ACGA_G Cuts at: 0 775 1593 Size: 775 818 BstAPI GCAn_nnn'nTGC Cuts at: 0 96 1593 Size: 96 1497 BstYI r'GATC_y Cuts at: 0 318 1593 Size: 318 1275 BstZ17I GTA'TAC Cuts at: 0 1058 1593 Size: 1058 535 BtgI C'CryG_G Cuts at: 0 639 1238 1593 Size: 639 599 355 Fragments arranged by size: 639 599 355 BtsI GCAGTG_nn' Cuts at: 0 1175 1593 Size: 1175 418 EaeI y'GGCC_r Cuts at: 0 452 530 1593 Size: 452 78 1063 Fragments arranged by size: 1063 452 78 EarI CTCTTCn'nnn_ Cuts at: 0 470 568 755 1064 1593 Size: 470 98 187 309 529 Fragments arranged by size: 529 470 309 187 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 331 1593 Size: 331 1262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 173 353 376 380 1360 1593 Size: 173 180 23 4 980 233 Fragments arranged by size: 980 233 180 173 23 4 EcoO109I rG'GnC_Cy Cuts at: 0 115 387 1593 Size: 115 272 1206 Fragments arranged by size: 1206 272 115 EcoRI G'AATT_C Cuts at: 0 732 999 1593 Size: 732 267 594 Fragments arranged by size: 732 594 267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1098 1593 Size: 1098 495 FspI TGC'GCA Cuts at: 0 940 1593 Size: 940 653 HaeII r_GCGC'y Cuts at: 0 58 152 1593 Size: 58 94 1441 Fragments arranged by size: 1441 94 58 HincII GTy'rAC Cuts at: 0 66 1593 Size: 66 1527 HindIII A'AGCT_T Cuts at: 0 612 1593 Size: 612 981 MscI TGG'CCA Cuts at: 0 454 532 1593 Size: 454 78 1061 Fragments arranged by size: 1061 454 78 MslI CAynn'nnrTG Cuts at: 0 133 583 1593 Size: 133 450 1010 Fragments arranged by size: 1010 450 133 NsiI A_TGCA'T Cuts at: 0 684 688 1329 1593 Size: 684 4 641 264 Fragments arranged by size: 684 641 264 4 NspI r_CATG'y Cuts at: 0 686 1039 1593 Size: 686 353 554 Fragments arranged by size: 686 554 353 PflMI CCAn_nnn'nTGG Cuts at: 0 1231 1593 Size: 1231 362 PshAI GACnn'nnGTC Cuts at: 0 1016 1593 Size: 1016 577 PspOMI G'GGCC_C Cuts at: 0 115 1593 Size: 115 1478 PstI C_TGCA'G Cuts at: 0 92 1593 Size: 92 1501 SfcI C'TryA_G Cuts at: 0 88 512 1593 Size: 88 424 1081 Fragments arranged by size: 1081 424 88 SfiI GGCCn_nnn'nGGCC Cuts at: 0 37 1593 Size: 37 1556 SmlI C'TyrA_G Cuts at: 0 1182 1593 Size: 1182 411 SphI G_CATG'C Cuts at: 0 686 1593 Size: 686 907 StuI AGG'CCT Cuts at: 0 338 1593 Size: 338 1255 StyI C'CwwG_G Cuts at: 0 26 119 1343 1593 Size: 26 93 1224 250 Fragments arranged by size: 1224 250 93 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 227 1420 1593 Size: 227 1193 173 Fragments arranged by size: 1193 227 173 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 828 860 1593 Size: 828 32 733 Fragments arranged by size: 828 733 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 33 1545 1593 Size: 33 1512 48 Fragments arranged by size: 1512 48 33 XmnI GAAnn'nnTTC Cuts at: 0 338 1578 1593 Size: 338 1240 15 Fragments arranged by size: 1240 338 15 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaI ApoI AseI AvaI BanI BanII BbsI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsgI BsiHKAI Bsp1286I BspHI BsrDI BssSI BstAPI BstYI BstZ17I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII HincII HindIII MscI MslI NsiI NspI PflMI PshAI PspOMI PstI SfcI SfiI SmlI SphI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglII BlpI BmtI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FseI FspAI Hin4I HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI