##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MamI        
MboI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccII         117  CGCG                 CG^CG
AfaI          252  GTAC                 GT^AC
AflIII        254  ACGTGT               A^C[GA][CT]GT
AlwNI         165  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         243  GGTCC                G^G[AT]CC
BanII          78  GGGCTC               G[GA]GC[CT]^C
BmyI           78  GGGCTC               G[AGT]GC[ACT]^C
BsaAI         253  TACGTG               [CT]AC^GT[GA]
BsaI          157  GAGACC               GGTCTC(1/5)
BsaJI         239  CCTGGG               C^C[ACGT][ACGT]GG
BseRI          28  CTCCTC               GAGGAG(10/8)
Bsp1286I       78  GGGCTC               G[AGT]GC[ACT]^C
BsrI          230  CCAGT                ACTGG(1/-1)
BssSI          31  CTCGTG               CACGAG(-5/-1)
BstAPI        164  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         117  CGCG                 CG^CG
CfoI          221  GCGC                 GCG^C
Cfr13I        243  GGTCC                G^G[ACGT]CC
DrdI           15  GACTGTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI          221  GCGC                 GCG^C
HinP1I        221  GCGC                 G^CGC
HindIII       124  AAGCTT               A^AGCTT
HinfI         235  GAGTC                G^A[ACGT]TC
MaeII         254  ACGT                 A^CGT
MaeIII         13  GTGAC                ^GT[ACGT]AC
MspA1I        182  CAGCTG               C[AC]G^C[GT]G
MvnI          117  CGCG                 CG^CG
NlaIV         242  GGGTCC               GG[ACGT]^[ACGT]CC
PleI          235  GAGTC                GAGTC(4/5)
PvuII         182  CAGCTG               CAG^CTG
RsaI          252  GTAC                 GT^AC
Sau96I        243  GGTCC                G^G[ACGT]CC
Tsp45I         13  GTGAC                ^GT[GC]AC
TspRI         231  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AciI           95  CCGC                 CCGC(-3/-1)
              116  CCGC                
BfaI           37  CTAG                 C^TAG
               58  CTAG                
BsmAI         157  GAGAC                GTCTC(1/5)
               24  GTCTC               
DdeI           66  CTCAG                C^T[ACGT]AG
               81  CTCAG               
MaeI           37  CTAG                 C^TAG
               58  CTAG                
MwoI           61  GCACTCTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              164  GCAGCCTCTGC         


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        BssKI       BstNI       Cac8I       
EcoRII      Fnu4HI      ItaI        MnlI        MvaI        
ScrFI       TseI