(Circular) (Six-base) MAPSORT of: pFN21AE0407.seq Check: 2210 from: 1 to: 5467 pFN21AE0407 5467 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2810 1937 Size: 873 4594 Acc65I G'GTAC_C Cuts at: 2788 2788 Size: 5467 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3530 4914 5182 1632 Size: 1898 1384 268 1917 Fragments arranged by size: 1917 1898 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 4366 5343 1203 Size: 3163 977 1327 Fragments arranged by size: 3163 1327 977 AgeI A'CCGG_T Cuts at: 4298 4298 Size: 5467 AhdI GACnn_n'nnGTC Cuts at: 4196 4196 Size: 5467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5467 AlwNI CAG_nnn'CTG Cuts at: 3210 4782 3210 Size: 1572 3895 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2402 2585 2776 3002 3154 5263 1493 Size: 909 183 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 909 226 191 183 152 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2792 1870 Size: 73 849 4545 Fragments arranged by size: 4545 849 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2238 2271 2238 Size: 33 5434 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 4243 618 Size: 325 217 627 1001 1455 1842 Fragments arranged by size: 1842 1455 1001 627 325 217 BanII G_rGCy'C Cuts at: 1391 2786 1391 Size: 1395 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2284 2593 3359 4569 2284 Size: 309 766 1210 3182 Fragments arranged by size: 3182 1210 766 309 BclI T'GATC_A Cuts at: 1548 1548 Size: 5467 BfrBI ATG'CAT Cuts at: 2198 2473 2198 Size: 275 5192 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 4078 136 Size: 107 122 71 631 605 405 2001 1525 Fragments arranged by size: 2001 1525 631 605 405 122 107 71 BglII A'GATC_T Cuts at: 5462 5462 Size: 5467 BlpI GC'TnA_GC Cuts at: 2884 2884 Size: 5467 Bme1580I G_kGCm'C Cuts at: 1790 3528 4684 1790 Size: 1738 1156 2573 Fragments arranged by size: 2573 1738 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 4156 442 Size: 416 53 83 399 24 1781 958 1753 Fragments arranged by size: 1781 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4127 1239 Size: 2888 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2301 2711 3592 4457 4755 2301 Size: 410 881 865 298 3013 Fragments arranged by size: 3013 881 865 410 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2621 4130 882 Size: 617 1122 1509 2219 Fragments arranged by size: 2219 1509 1122 617 BsaAI yAC'GTr Cuts at: 493 1204 3258 493 Size: 711 2054 2702 Fragments arranged by size: 2702 2054 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3274 3656 4291 275 Size: 53 83 186 936 1741 382 635 1451 Fragments arranged by size: 1741 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3900 4298 4572 4719 5130 3900 Size: 398 274 147 411 4237 Fragments arranged by size: 4237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5242 Fragments arranged by size: 5242 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2064 1186 Size: 878 4589 BseYI C'CCAG_C Cuts at: 4670 5166 4670 Size: 496 4971 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4894 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3528 3613 4684 3528 Size: 85 1071 4311 Fragments arranged by size: 4311 1071 85 BsmI GAATG_Cn' Cuts at: 2978 3071 2978 Size: 93 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2786 3528 3613 4684 1391 Size: 399 996 742 85 1071 2174 Fragments arranged by size: 2174 1071 996 742 399 85 BspEI T'CCGG_A Cuts at: 5130 5130 Size: 5467 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2822 844 Size: 1978 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3354 1394 Size: 429 1531 3507 Fragments arranged by size: 3507 1531 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3962 4136 65 Size: 1183 2714 174 1396 Fragments arranged by size: 2714 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 4111 4298 1700 Size: 110 279 2022 187 2869 Fragments arranged by size: 2869 2022 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 3527 4539 3527 Size: 1012 4455 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5467 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2797 3164 3549 3566 5018 5104 5116 5462 Size: 825 367 385 17 1452 86 12 346 Cuts at: 5462 1972 Size: 1977 Fragments arranged by size: 1977 1452 825 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2074 513 Size: 552 1009 3906 Fragments arranged by size: 3906 1009 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2983 3777 3797 2983 Size: 794 20 4653 Fragments arranged by size: 4653 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 2914 3803 Size: 54 1425 50 275 18 314 770 889 Cuts at: 3803 5432 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 770 314 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3397 1353 Size: 2044 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4054 4428 4574 387 Size: 1064 138 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3530 4127 4914 5182 1239 Size: 393 1898 597 787 268 1524 Fragments arranged by size: 1898 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2070 2070 Size: 5467 EcoRI G'AATT_C Cuts at: 2776 3154 2776 Size: 378 5089 EcoRV GAT'ATC Cuts at: 2512 2512 Size: 5467 FspI TGC'GCA Cuts at: 3973 3973 Size: 5467 HaeII r_GCGC'y Cuts at: 1678 4614 1678 Size: 2936 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2299 2331 4108 4140 4182 Size: 32 135 32 727 32 1777 32 42 Cuts at: 4182 4214 1373 Size: 32 2626 Fragments arranged by size: 2626 1777 727 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2265 2811 3057 669 Size: 988 281 327 546 246 3079 Fragments arranged by size: 3079 988 546 327 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 2265 3057 2265 Size: 792 4675 KpnI G_GTAC'C Cuts at: 2792 2792 Size: 5467 MfeI C'AATT_G Cuts at: 2250 3066 2250 Size: 816 4651 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4581 4765 1305 Size: 3276 184 2007 Fragments arranged by size: 3276 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3645 Fragments arranged by size: 3645 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2361 3425 3784 3943 518 Size: 648 1195 1064 359 159 2042 Fragments arranged by size: 2042 1195 1064 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2883 3560 4708 4953 1606 Size: 470 807 677 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 470 245 NaeI GCC'GGC Cuts at: 2091 2091 Size: 5467 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4915 NdeI CA'TA_TG Cuts at: 387 387 Size: 5467 NgoMIV G'CCGG_C Cuts at: 2089 2089 Size: 5467 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NsiI A_TGCA'T Cuts at: 2200 2475 2200 Size: 275 5192 NspI r_CATG'y Cuts at: 1132 1229 2825 4370 1132 Size: 97 1596 1545 2229 Fragments arranged by size: 2229 1596 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5467 PciI A'CATG_T Cuts at: 4366 4366 Size: 5467 PflMI CCAn_nnn'nTGG Cuts at: 1108 2200 1108 Size: 1092 4375 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PstI C_TGCA'G Cuts at: 830 2819 830 Size: 1989 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 SacII CC_GC'GG Cuts at: 2077 2077 Size: 5467 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2580 2815 3950 4631 4822 826 Size: 219 1535 235 1135 681 191 1471 Fragments arranged by size: 1535 1471 1135 681 235 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5467 SmaI CCC'GGG Cuts at: 1872 2794 1872 Size: 922 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2316 2690 3571 4472 4734 820 Size: 197 926 373 374 881 901 262 1553 Fragments arranged by size: 1553 926 901 881 374 373 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5467 StyI C'CwwG_G Cuts at: 513 1065 2041 2124 2906 513 Size: 552 976 83 782 3074 Fragments arranged by size: 3074 976 782 552 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 3511 3664 3849 4294 325 Size: 1255 1914 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2338 3713 5425 Size: 274 80 33 51 1443 361 1375 1712 Cuts at: 5425 96 Size: 138 Fragments arranged by size: 1712 1443 1375 361 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5427 5459 5427 Size: 32 5435 Tth111I GACn'n_nGTC Cuts at: 1316 2381 1316 Size: 1065 4402 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2792 1870 Size: 922 4545 XmnI GAAnn'nnTTC Cuts at: 3596 3596 Size: 5467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI