Restriction map for
pFN21AB6447
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BseRI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 316 CGCG CG^CG
AccIII 719 TCCGGA T^CCGGA
AcyI 105 GGCGTC G[GA]^CG[CT]C
AgeI 671 ACCGGT A^CCGGT
Asp700I 570 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 412 GAGCAC G[AT]GC[AT]^C
BalI 560 TGGCCA TGG^CCA
BamHI 311 GGATCC G^GATCC
BanI 547 GGCACC G^G[CT][GA]CC
BfaI 466 CTAG C^TAG
BlpI 423 GCTGAGC GC^T[ACGT]AGC
BmyI 412 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
BsaHI 105 GGCGTC G[GA]^CG[CT]C
BsaJI 488 CCGAGG C^C[ACGT][ACGT]GG
BseAI 719 TCCGGA T^CCGGA
BsgI 175 GTGCAG GTGCAG(16/14)
BsiEI 673 CGGTCG CG[GA][CT]^CG
BsiHKAI 412 GAGCAC G[AT]GC[AT]^C
BsmAI 148 GAGAC GTCTC(1/5)
BsmFI 114 GTCCC GGGAC(10/14)
Bsp1286I 412 GAGCAC G[AGT]GC[ACT]^C
BspEI 719 TCCGGA T^CCGGA
BspMI 218 ACCTGC ACCTGC(4/8)
BsrBI 61 GAGCGG CCGCTC(-3/-3)
BsrFI 671 ACCGGT [GA]^CCGG[CT]
BstUI 316 CGCG CG^CG
BstYI 311 GGATCC [GA]^GATC[CT]
CelII 423 GCTGAGC GC^T[ACGT]AGC
Cfr10I 671 ACCGGT [GA]^CCGG[CT]
EcoT22I 160 ATGCAT ATGCA^T
HgaI 106 GCGTC GACGC(5/10)
Hin1I 105 GGCGTC G[GA]^CG[CT]C
MaeI 466 CTAG C^TAG
MaeIII 607 GTGAC ^GT[ACGT]AC
MflI 311 GGATCC [GA]^GATC[CT]
MluNI 560 TGGCCA TGG^CCA
MroI 719 TCCGGA T^CCGGA
MscI 560 TGGCCA TGG^CCA
MslI 552 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 316 CGCG CG^CG
NsiI 160 ATGCAT ATGCA^T
NspI 362 ACATGC [GA]CATG^[CT]
PinAI 671 ACCGGT A^CCGGT
PvuII 640 CAGCTG CAG^CTG
ScaI 38 AGTACT AGT^ACT
SmlI 349 CTCAAG C^T[CT][GA]AG
TfiI 493 GAATC G^A[AT]TC
Tsp45I 607 GTGAC ^GT[GC]AC
Tsp509I 659 AATT ^AATT
TspRI 620 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 122 CCATCTGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 311 GGATCC [GA]^GATC[CT]
XmnI 570 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 39 GTAC GT^AC
462 GTAC
AlwNI 367 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
418 CAGCGGCTG
BbsI 25 GAAGAC GAAGAC(2/6)
531 GAAGAC
BpuAI 25 GAAGAC GAAGAC(2/6)
531 GAAGAC
BsaWI 671 ACCGGT [AT]^CCGG[AT]
719 TCCGGA
BssKI 30 CCTGG ^CC[ACGT]GG
291 CCAGG
BstNI 30 CCTGG CC^[AT]GG
291 CCAGG
BstXI 118 CCATCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCATTAAGATGG
EaeI 32 TGGCCG [CT]^GGCC[GA]
560 TGGCCA
EarI 574 CTCTTC CTCTTC(1/4)
19 GAAGAG
EcoNI 465 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
597 CCTGAAGGAGG
EcoRII 30 CCTGG ^CC[AT]GG
291 CCAGG
HaeII 399 GGCGCT [GA]GCGC^[CT]
427 AGCGCC
HaeIII 33 GGCC GG^CC
561 GGCC
HphI 606 GGTGA GGTGA(8/7)
693 GGTGA
Ksp632I 574 CTCTTC CTCTTC(1/4)
19 GAAGAG
MseI 242 TTAA T^TAA
554 TTAA
MspA1I 418 CAGCGG C[AC]G^C[GT]G
640 CAGCTG
MvaI 30 CCTGG CC^[AT]GG
291 CCAGG
NlaIV 311 GGATCC GG[ACGT]^[ACGT]CC
547 GGCACC
PstI 49 CTGCAG CTGCA^G
70 CTGCAG
RsaI 39 GTAC GT^AC
462 GTAC
ScrFI 30 CCTGG CC^[ACGT]GG
291 CCAGG
SfcI 49 CTGCAG C^T[GA][CT]AG
70 CTGCAG
Tru9I 242 TTAA T^TAA
554 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwI BbvI BpmI
BsiYI BslI BsrI BstF5I Cac8I
CfoI DdeI DpnII Fnu4HI FokI
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MnlI MspI
MwoI NdeII NlaIII PleI Sau3AI
SfaNI TseI