(Circular) (Six-base) MAPSORT of: pFN21AB6447.seq Check: 3704 from: 1 to: 5431 pFN21AB6447 5431 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 2462 2624 3494 4878 5146 1632 Size: 650 180 162 870 1384 268 1917 Fragments arranged by size: 1917 1384 870 650 268 180 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 2676 4262 2676 Size: 1586 3845 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 2377 2428 3174 4746 2377 Size: 51 746 1572 3062 Fragments arranged by size: 3062 1572 746 51 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2740 2966 3118 5227 1493 Size: 1247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1247 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2756 1870 Size: 73 813 4545 Fragments arranged by size: 4545 813 73 BamHI G'GATC_C Cuts at: 2316 2761 3128 2316 Size: 445 367 4619 Fragments arranged by size: 4619 445 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2552 2752 4207 618 Size: 325 217 627 765 200 1455 1842 Fragments arranged by size: 1842 1455 765 627 325 217 200 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2543 928 Size: 971 138 506 3816 Fragments arranged by size: 3816 971 506 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 1903 Size: 34 1685 34 3678 Fragments arranged by size: 3678 1685 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BfrBI ATG'CAT Cuts at: 2167 2167 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2429 2848 2429 Size: 419 5012 Bme1580I G_kGCm'C Cuts at: 1790 3492 4648 1790 Size: 1702 1156 2573 Fragments arranged by size: 2573 1702 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2249 3162 4120 Size: 416 53 83 399 24 832 913 958 Cuts at: 4120 442 Size: 1753 Fragments arranged by size: 1753 958 913 832 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2159 2435 2648 4091 1239 Size: 830 90 276 213 1443 2579 Fragments arranged by size: 2579 1443 830 276 213 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 3556 4421 4719 2339 Size: 1217 865 298 3051 Fragments arranged by size: 3051 1217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4094 882 Size: 617 2595 2219 Fragments arranged by size: 2595 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 3238 3620 4255 Size: 53 83 186 936 578 1127 382 635 Cuts at: 4255 275 Size: 1451 Fragments arranged by size: 1451 1127 936 635 578 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2676 2724 3864 4262 4536 4683 5094 2676 Size: 48 1140 398 274 147 411 3013 Fragments arranged by size: 3013 1140 411 398 274 147 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5206 Fragments arranged by size: 5206 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5431 BseYI C'CCAG_C Cuts at: 2229 2505 4634 5130 2229 Size: 276 2129 496 2530 Fragments arranged by size: 2530 2129 496 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 1163 Size: 573 465 4393 Fragments arranged by size: 4393 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2681 2881 3642 4670 1540 Size: 275 186 680 200 761 1028 2301 Fragments arranged by size: 2301 1028 761 680 275 200 186 BsiHKAI G_wGCw'C Cuts at: 2421 3492 3577 4648 2421 Size: 1071 85 1071 3204 Fragments arranged by size: 3204 1071 1071 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2421 2750 3492 3577 4648 1391 Size: 399 631 329 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 631 399 329 85 BspEI T'CCGG_A Cuts at: 2724 5094 2724 Size: 2370 3061 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 2786 844 Size: 1388 554 3489 Fragments arranged by size: 3489 1388 554 BsrBI CCG'CTC Cuts at: 1394 1823 2068 3318 1394 Size: 429 245 1250 3507 Fragments arranged by size: 3507 1250 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2676 4075 4262 1700 Size: 110 866 1399 187 2869 Fragments arranged by size: 2869 1399 866 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2130 2563 2011 Size: 119 433 4879 Fragments arranged by size: 4879 433 119 BstYI r'GATC_y Cuts at: 1972 2316 2761 3128 3513 3530 4982 5068 5080 Size: 344 445 367 385 17 1452 86 12 Cuts at: 5080 5426 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 445 385 367 346 344 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4879 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3741 3761 2947 Size: 794 20 4617 Fragments arranged by size: 4617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2565 2878 Size: 54 1425 50 275 18 207 528 313 Cuts at: 2878 3767 5396 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 528 313 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2585 3361 1353 Size: 666 566 776 3423 Fragments arranged by size: 3423 776 666 566 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4018 4392 4538 387 Size: 1064 138 2429 374 146 1280 Fragments arranged by size: 2429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2159 2282 2435 2462 2624 2648 Size: 393 437 90 123 153 27 162 24 Cuts at: 2648 3494 4091 4878 5146 1239 Size: 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 437 393 268 162 153 123 90 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 2474 2606 2474 Size: 132 5299 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2408 2436 4578 1678 Size: 730 28 2142 2531 Fragments arranged by size: 2531 2142 730 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2108 2128 2140 4072 Size: 32 135 32 524 12 20 12 1932 Cuts at: 4072 4104 4146 4178 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1932 524 135 42 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2661 4545 4729 1305 Size: 789 567 1884 184 2007 Fragments arranged by size: 2007 1884 789 567 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 10 Size: 54 1425 343 735 2874 Fragments arranged by size: 2874 1425 735 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 3389 3748 3907 518 Size: 648 1395 828 359 159 2042 Fragments arranged by size: 2042 1395 828 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2425 2647 2847 3524 4672 4917 1606 Size: 819 222 200 677 1148 245 2120 Fragments arranged by size: 2120 1148 819 677 245 222 200 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4879 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NsiI A_TGCA'T Cuts at: 2169 2169 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2371 2789 4334 1132 Size: 97 1142 418 1545 2229 Fragments arranged by size: 2229 1545 1142 418 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PfoI T'CCnGG_A Cuts at: 2295 2295 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PstI C_TGCA'G Cuts at: 830 2058 2079 2783 830 Size: 1228 21 704 3478 Fragments arranged by size: 3478 1228 704 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 PvuII CAG'CTG Cuts at: 2647 2647 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 2045 3679 5391 2045 Size: 1634 1712 2085 Fragments arranged by size: 2085 1712 1634 SfcI C'TryA_G Cuts at: 826 1045 2054 2075 2779 3914 4595 4786 826 Size: 219 1009 21 704 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 704 681 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 3535 4436 4698 820 Size: 197 926 411 1181 901 262 1553 Fragments arranged by size: 1553 1181 926 901 411 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2870 513 Size: 552 1805 3074 Fragments arranged by size: 3074 1805 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 3458 3475 3628 3813 4258 325 Size: 1255 1071 807 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1071 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2043 3677 5389 Size: 274 80 33 51 1443 66 1634 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1634 1443 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5391 5423 5391 Size: 32 5399 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2134 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 2579 3560 2579 Size: 981 4450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI