(Circular) (Six-base) MAPSORT of: pFN21AE0514.seq Check: 5430 from: 1 to: 5392 pFN21AE0514 5392 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2735 1937 Size: 798 4594 Acc65I G'GTAC_C Cuts at: 2713 2713 Size: 5392 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2327 3455 4839 5107 1632 Size: 542 153 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1128 542 268 153 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 4291 5268 1203 Size: 3088 977 1327 Fragments arranged by size: 3088 1327 977 AgeI A'CCGG_T Cuts at: 4223 4223 Size: 5392 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5392 AlwNI CAG_nnn'CTG Cuts at: 2442 2509 3135 4707 2442 Size: 67 626 1572 3127 Fragments arranged by size: 3127 1572 626 67 ApaI G_GGCC'C Cuts at: 2286 2475 2286 Size: 189 5203 ApaLI G'TGCA_C Cuts at: 2381 3449 4605 2381 Size: 1068 1156 3168 Fragments arranged by size: 3168 1156 1068 ApoI r'AATT_y Cuts at: 1493 2701 2927 3079 5188 1493 Size: 1208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1208 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2371 2717 1870 Size: 73 428 346 4545 Fragments arranged by size: 4545 428 346 73 BamHI G'GATC_C Cuts at: 2722 3089 2722 Size: 367 5025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2713 4168 618 Size: 325 217 627 282 644 1455 1842 Fragments arranged by size: 1842 1455 644 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 2223 2286 2475 2711 1391 Size: 832 63 189 236 4072 Fragments arranged by size: 4072 832 236 189 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2452 928 Size: 971 553 3868 Fragments arranged by size: 3868 971 553 BbvCI CC'TCA_GC Cuts at: 2489 2489 Size: 5392 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 3583 3617 1903 Size: 34 425 34 1187 34 3678 Fragments arranged by size: 3678 1187 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4494 3284 Size: 1210 4182 BclI T'GATC_A Cuts at: 1548 1548 Size: 5392 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4003 136 Size: 107 122 71 631 605 2331 1525 Fragments arranged by size: 2331 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2103 5387 2103 Size: 3284 2108 BlpI GC'TnA_GC Cuts at: 2809 2809 Size: 5392 Bme1580I G_kGCm'C Cuts at: 1790 2286 2385 2475 3453 4609 1790 Size: 496 99 90 978 1156 2573 Fragments arranged by size: 2573 1156 978 496 99 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2674 3123 4081 Size: 416 53 83 399 24 1257 449 958 Cuts at: 4081 442 Size: 1753 Fragments arranged by size: 1753 1257 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2255 2656 4052 1239 Size: 881 135 401 1396 2579 Fragments arranged by size: 2579 1396 881 401 135 Bpu10I CC'TnA_GC Cuts at: 2489 2489 Size: 5392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2095 2198 3517 4382 4680 2095 Size: 103 1319 865 298 2807 Fragments arranged by size: 2807 1319 865 298 103 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4055 882 Size: 617 2556 2219 Fragments arranged by size: 2556 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2326 3183 493 Size: 711 1122 857 2702 Fragments arranged by size: 2702 1122 857 711 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2457 3199 3581 4216 Size: 53 83 186 936 924 742 382 635 Cuts at: 4216 275 Size: 1451 Fragments arranged by size: 1451 936 924 742 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3825 4223 4497 4644 5055 3825 Size: 398 274 147 411 4162 Fragments arranged by size: 4162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2387 2417 1178 Size: 30 165 30 726 30 228 30 4153 Fragments arranged by size: 4153 726 228 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2648 1186 Size: 1222 240 3930 Fragments arranged by size: 3930 1222 240 BseYI C'CCAG_C Cuts at: 2427 2509 2576 4595 5091 2427 Size: 82 67 2019 496 2728 Fragments arranged by size: 2728 2019 496 82 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4819 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2560 2842 3603 4631 1540 Size: 275 186 559 282 761 1028 2301 Fragments arranged by size: 2301 1028 761 559 282 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2274 2385 3453 3538 4609 2256 Size: 18 111 1068 85 1071 3039 Fragments arranged by size: 3039 1071 1068 111 85 18 BsmI GAATG_Cn' Cuts at: 2903 2996 2903 Size: 93 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2256 2274 2286 2385 2475 2711 Size: 399 433 33 18 12 99 90 236 Cuts at: 2711 3453 3538 4609 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 236 99 90 85 33 18 12 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 3281 5011 3281 Size: 1730 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 844 Size: 1903 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3279 1394 Size: 429 1456 3507 Fragments arranged by size: 3507 1456 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3887 4061 65 Size: 1183 2639 174 1396 Fragments arranged by size: 2639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 4223 1700 Size: 110 2226 187 2869 Fragments arranged by size: 2869 2226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5392 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5392 BssSI C'ACGA_G Cuts at: 3452 4464 3452 Size: 1012 4380 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5392 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstYI r'GATC_y Cuts at: 1972 2103 2196 2649 2667 2675 2722 3089 3474 Size: 131 93 453 18 8 47 367 385 Cuts at: 3474 3491 4943 5029 5041 5387 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 453 385 367 346 131 93 86 47 18 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2367 487 Size: 683 56 1141 3512 Fragments arranged by size: 3512 1141 683 56 BtsI GCAGTG_nn' Cuts at: 2908 3702 3722 2908 Size: 794 20 4578 Fragments arranged by size: 4578 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2551 2696 2551 Size: 145 5247 DraIII CAC_nnn'GTG Cuts at: 2329 2329 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4399 809 Size: 507 3083 1802 Fragments arranged by size: 3083 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357 Size: 54 1425 50 275 18 1009 889 1629 Cuts at: 5357 8 Size: 43 Fragments arranged by size: 1629 1425 1009 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 1537 Size: 275 1027 4090 Fragments arranged by size: 4090 1027 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 3322 1353 Size: 1254 715 3423 Fragments arranged by size: 3423 1254 715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3979 4353 4499 387 Size: 1064 138 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2174 2255 2327 2656 3455 4052 Size: 393 488 54 81 72 329 799 597 Cuts at: 4052 4839 5107 1239 Size: 787 268 1524 Fragments arranged by size: 1524 799 787 597 488 393 329 268 81 72 54 EcoO109I rG'GnC_Cy Cuts at: 2079 2283 2471 2472 2599 2079 Size: 204 188 1 127 4872 Fragments arranged by size: 4872 204 188 127 1 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2239 Size: 5392 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 4539 1678 Size: 2861 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2387 2419 4033 Size: 32 135 32 731 32 52 32 1614 Cuts at: 4033 4065 4107 4139 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1614 731 135 52 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5392 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2717 2717 Size: 5392 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 2556 4506 4690 1305 Size: 1125 126 1950 184 2007 Fragments arranged by size: 2007 1950 1125 184 126 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3570 Fragments arranged by size: 3570 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 3709 3868 518 Size: 648 2184 359 159 2042 Fragments arranged by size: 2184 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2170 2261 2427 2808 3485 4633 4878 Size: 537 27 91 166 381 677 1148 245 Cuts at: 4878 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 537 381 245 166 91 27 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2839 2839 Size: 5392 NspI r_CATG'y Cuts at: 1132 1229 2750 4295 1132 Size: 97 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5392 PciI A'CATG_T Cuts at: 4291 4291 Size: 5392 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 2509 1108 Size: 1221 180 3991 Fragments arranged by size: 3991 1221 180 PfoI T'CCnGG_A Cuts at: 2243 2243 Size: 5392 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PmlI CAC'GTG Cuts at: 2326 2326 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3461 3493 4974 5006 3461 Size: 32 1481 32 3847 Fragments arranged by size: 3847 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 2079 Size: 5392 PshAI GACnn'nnGTC Cuts at: 1613 2456 1613 Size: 843 4549 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PspOMI G'GGCC_C Cuts at: 2282 2471 2282 Size: 189 5203 PstI C_TGCA'G Cuts at: 830 2744 830 Size: 1914 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5392 PvuII CAG'CTG Cuts at: 2143 2170 2143 Size: 27 5365 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 3640 5352 3640 Size: 1712 3680 SfcI C'TryA_G Cuts at: 826 1045 2740 3875 4556 4747 826 Size: 219 1695 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2719 1872 Size: 847 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 2213 3496 4397 4659 820 Size: 197 926 131 139 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 901 262 197 139 131 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2750 1132 Size: 1618 3774 SspI AAT'ATT Cuts at: 5 52 3316 5 Size: 47 3264 2081 Fragments arranged by size: 3264 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2004 2663 2831 513 Size: 552 939 659 168 3074 Fragments arranged by size: 3074 939 659 552 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3419 3436 3589 3774 4219 325 Size: 1255 1839 17 153 185 445 1498 Fragments arranged by size: 1839 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 3638 5350 Size: 274 80 33 51 1443 213 1448 1712 Cuts at: 5350 96 Size: 138 Fragments arranged by size: 1712 1448 1443 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 5352 Size: 32 5360 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5392 XmaI C'CCGG_G Cuts at: 1870 2717 1870 Size: 847 4545 XmnI GAAnn'nnTTC Cuts at: 3521 3521 Size: 5392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI