Restriction map for
pFN21AE0514
(Linear) (Six-base) MAPSORT of: KIEE0514.seq Check: 2062 from: 1 to: 687
KIEE0514.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 322 687
Size: 169 153 365
Fragments arranged by size:
365 169 153
AlwNI CAG_nnn'CTG
Cuts at: 0 437 504 687
Size: 437 67 183
Fragments arranged by size:
437 183 67
ApaI G_GGCC'C
Cuts at: 0 281 470 687
Size: 281 189 217
Fragments arranged by size:
281 217 189
ApaLI G'TGCA_C
Cuts at: 0 376 687
Size: 376 311
AvaI C'yCGr_G
Cuts at: 0 366 687
Size: 366 321
BanI G'GyrC_C
Cuts at: 0 64 687
Size: 64 623
BanII G_rGCy'C
Cuts at: 0 218 281 470 687
Size: 218 63 189 217
Fragments arranged by size:
218 217 189 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 687
Size: 447 240
BbvCI CC'TCA_GC
Cuts at: 0 484 687
Size: 484 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 687
Size: 357 34 296
Fragments arranged by size:
357 296 34
BglII A'GATC_T
Cuts at: 0 98 687
Size: 98 589
Bme1580I G_kGCm'C
Cuts at: 0 281 380 470 687
Size: 281 99 90 217
Fragments arranged by size:
281 217 99 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 687
Size: 669 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 540 687
Size: 540 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 250 651 687
Size: 115 135 401 36
Fragments arranged by size:
401 135 115 36
Bpu10I CC'TnA_GC
Cuts at: 0 484 687
Size: 484 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 193 687
Size: 90 103 494
Fragments arranged by size:
494 103 90
BsaAI yAC'GTr
Cuts at: 0 321 687
Size: 321 366
BsaHI Gr'CG_yC
Cuts at: 0 452 687
Size: 452 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 382 412 687
Size: 124 30 228 30 275
Fragments arranged by size:
275 228 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 643 687
Size: 403 240 44
Fragments arranged by size:
403 240 44
BseYI C'CCAG_C
Cuts at: 0 422 504 571 687
Size: 422 82 67 116
Fragments arranged by size:
422 116 82 67
BsiEI CG_ry'CG
Cuts at: 0 555 687
Size: 555 132
BsiHKAI G_wGCw'C
Cuts at: 0 251 269 380 687
Size: 251 18 111 307
Fragments arranged by size:
307 251 111 18
Bsp1286I G_dGCh'C
Cuts at: 0 218 251 269 281 380 470 687
Size: 218 33 18 12 99 90 217
Fragments arranged by size:
218 217 99 90 33 18 12
BstYI r'GATC_y
Cuts at: 0 98 191 644 662 670 687
Size: 98 93 453 18 8 17
Fragments arranged by size:
453 98 93 18 17 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 362 687
Size: 362 325
DraI TTT'AAA
Cuts at: 0 546 687
Size: 546 141
DraIII CAC_nnn'GTG
Cuts at: 0 324 687
Size: 324 363
EarI CTCTTCn'nnn_
Cuts at: 0 602 687
Size: 602 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 169 250 322 651 687
Size: 115 54 81 72 329 36
Fragments arranged by size:
329 115 81 72 54 36
EcoO109I rG'GnC_Cy
Cuts at: 0 74 278 466 467 594 687
Size: 74 204 188 1 127 93
Fragments arranged by size:
204 188 127 93 74 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 687
Size: 234 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 382 414 687
Size: 298 32 52 32 273
Fragments arranged by size:
298 273 52 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 551 687
Size: 425 126 136
Fragments arranged by size:
425 136 126
MspA1I CmG'CkG
Cuts at: 0 138 165 256 422 687
Size: 138 27 91 166 265
Fragments arranged by size:
265 166 138 91 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 504 687
Size: 324 180 183
Fragments arranged by size:
324 183 180
PfoI T'CCnGG_A
Cuts at: 0 238 687
Size: 238 449
PmlI CAC'GTG
Cuts at: 0 321 687
Size: 321 366
PpuMI rG'GwC_Cy
Cuts at: 0 74 687
Size: 74 613
PshAI GACnn'nnGTC
Cuts at: 0 451 687
Size: 451 236
PspOMI G'GGCC_C
Cuts at: 0 277 466 687
Size: 277 189 221
Fragments arranged by size:
277 221 189
PvuII CAG'CTG
Cuts at: 0 138 165 687
Size: 138 27 522
Fragments arranged by size:
522 138 27
SmlI C'TyrA_G
Cuts at: 0 69 208 687
Size: 69 139 479
Fragments arranged by size:
479 139 69
StyI C'CwwG_G
Cuts at: 0 658 687
Size: 658 29
TatI w'GTAC_w
Cuts at: 0 185 687
Size: 185 502
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI AvaI BanI BanII BbsI
BbvCI BcgI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BstYI BtgZI DraI DraIII EarI Eco57MI EcoO109I
FalI Hin4I MmeI MspA1I PflMI PfoI PmlI PpuMI
PshAI PspOMI PvuII SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BsaI BsaBI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PpiI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI