Restriction map for
pFN21AE0509
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FspI HaeII HgaI Hin1I
HphI KasI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 235 GAATTT [GA]^AATT[CT]
AlwI 72 GGATC GGATC(4/5)
ApoI 235 GAATTT [GA]^AATT[CT]
BfaI 485 CTAG C^TAG
BlpI 382 GCTAAGC GC^T[ACGT]AGC
BpmI 83 CTGGAG CTGGAG(16/14)
Bpu1102I 382 GCTAAGC GC^T[ACGT]AGC
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsrFI 14 GCCGGC [GA]^CCGG[CT]
BssKI 40 CCTGG ^CC[ACGT]GG
BstNI 40 CCTGG CC^[AT]GG
CelII 382 GCTAAGC GC^T[ACGT]AGC
CfoI 7 GCGC GCG^C
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
Cfr13I 17 GGCCC G^G[ACGT]CC
DpnII 73 GATC ^GATC
DraIII 92 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 287 CCATGG C^C[GA][CT]GG
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
EcoRII 40 CCTGG ^CC[AT]GG
EcoT14I 287 CCATGG C^C[AT][AT]GG
FseI 13 GGCCGGCC GGCCGG^CC
HapII 15 CCGG C^CGG
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HincII 178 GTTAAC GT[CT]^[GA]AC
HindII 178 GTTAAC GT[CT]^[GA]AC
HindIII 115 AAGCTT A^AGCTT
HinfI 268 GAGTC G^A[ACGT]TC
HpaI 178 GTTAAC GTT^AAC
HpaII 15 CCGG C^CGG
MaeI 485 CTAG C^TAG
MboI 73 GATC ^GATC
MseI 179 TTAA T^TAA
MspI 15 CCGG C^CGG
MvaI 40 CCTGG CC^[AT]GG
NaeI 14 GCCGGC GCC^GGC
NcoI 287 CCATGG C^CATGG
NdeII 73 GATC ^GATC
NgoMIV 14 GCCGGC G^CCGGC
NlaIV 435 GGAACC GG[ACGT]^[ACGT]CC
NspI 387 GCATGT [GA]CATG^[CT]
PleI 268 GAGTC GAGTC(4/5)
Sau3AI 73 GATC ^GATC
Sau96I 17 GGCCC G^G[ACGT]CC
ScrFI 40 CCTGG CC^[ACGT]GG
StyI 287 CCATGG C^C[AT][AT]GG
Tru9I 179 TTAA T^TAA
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbvI 4 GCTGC GCAGC(8/12)
394 GCTGC
BsiYI 19 CCCTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
35 CCATACCTGGG
BslI 19 CCCTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
35 CCATACCTGGG
BsmAI 475 GAGAC GTCTC(1/5)
456 GTCTC
BsrI 82 ACTGG ACTGG(1/-1)
191 CCAGT
BstF5I 344 CATCC GGATG(2/0)
452 GGATG
Cac8I 14 GCCGGC GC[ACGT]^[ACGT]GC
397 GCTGGC
EaeI 12 CGGCCG [CT]^GGCC[GA]
442 CGGCCA
EarI 228 GAAGAG CTCTTC(1/4)
496 GAAGAG
Fnu4HI 4 GCTGC GC^[ACGT]GC
394 GCTGC
FokI 344 CATCC GGATG(9/13)
452 GGATG
ItaI 4 GCTGC GC^[ACGT]GC
394 GCTGC
Ksp632I 228 GAAGAG CTCTTC(1/4)
496 GAAGAG
MwoI 9 GCTCGGCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
397 GCTGGCACTGC
TseI 4 GCTGC G^C[AT]GC
394 GCTGC
Tsp509I 141 AATT ^AATT
236 AATT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AluI BsaJI DdeI HaeIII MboII
MnlI NlaIII TspRI