Restriction map for
pFN21AB5996
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BcgI BciVI BclI
BfrI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstAPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 988 CGCG CG^CG
AcyI 976 GGCGCC G[GA]^CG[CT]C
Alw44I 65 GTGCAC G^TGCAC
AlwNI 241 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1520 GGGCCC GGGCC^C
ApaLI 65 GTGCAC G^TGCAC
AvaI 21 CTCGGG C^[CT]CG[GA]G
AvrII 531 CCTAGG C^CTAGG
BbeI 976 GGCGCC GGCGC^C
BbrPI 1408 CACGTG CAC^GTG
BglII 1032 AGATCT A^GATCT
BlnI 531 CCTAGG C^CTAGG
BsaAI 1408 CACGTG [CT]AC^GT[GA]
BsaHI 976 GGCGCC G[GA]^CG[CT]C
BsaI 1474 GAGACC GGTCTC(1/5)
BsaWI 1481 ACCGGA [AT]^CCGG[AT]
BsoBI 21 CTCGGG C^[CT]CG[GA]G
BsrDI 548 CATTGC GCAATG(2/0)
BstBI 1357 TTCGAA TT^CGAA
BstEII 25 GGTTACC G^GT[ACGT]ACC
BstPI 25 GGTTACC G^GT[ACGT]ACC
BstUI 988 CGCG CG^CG
BstYI 1032 AGATCT [GA]^GATC[CT]
DsaI 972 CCGTGG C^C[GA][CT]GG
EarI 925 CTCTTC CTCTTC(1/4)
EcoNI 1290 CCTTTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 25 GGTTACC G^GT[ACGT]ACC
HaeII 976 GGCGCC [GA]GCGC^[CT]
Hin1I 976 GGCGCC G[GA]^CG[CT]C
HphI 1403 TCACC GGTGA(8/7)
KasI 976 GGCGCC G^GCGCC
Ksp632I 925 CTCTTC CTCTTC(1/4)
MaeII 1409 ACGT A^CGT
MflI 1032 AGATCT [GA]^GATC[CT]
MseI 1435 TTAA T^TAA
MvnI 988 CGCG CG^CG
NarI 976 GGCGCC GG^CGCC
NspV 1357 TTCGAA TT^CGAA
PmaCI 1408 CACGTG CAC^GTG
PmlI 1408 CACGTG CAC^GTG
PshAI 991 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1300 GAGCTC GAGCT^C
SfiI 1521 GGCCCGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 976 GGCGCC GGC^GCC
SfuI 1357 TTCGAA TT^CGAA
Tru9I 1435 TTAA T^TAA
Tsp45I 527 GTGAC ^GT[GC]AC
XcmI 83 CCAAGAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1032 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 151 ACATGT A^C[GA][CT]GT
1691 ACATGT
BbsI 300 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BbvCI 199 CCTCAGC CCTCAGC(-5/-2)
387 CCTCAGC
BfaI 532 CTAG C^TAG
1169 CTAG
BpuAI 300 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BsiEI 415 CGGCCG CG[GA][CT]^CG
993 CGGCCG
BspLU11I 151 ACATGT A^CATGT
1691 ACATGT
BspMI 860 ACCTGC ACCTGC(4/8)
1220 GCAGGT
BsrBI 877 CCGCTC CCGCTC(-3/-3)
1629 CCGCTC
BstXI 1010 CCACCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCAAGGACATGG
CfoI 285 GCGC GCG^C
977 GCGC
DpnII 825 GATC ^GATC
1033 GATC
EagI 415 CGGCCG C^GGCCG
993 CGGCCG
EclXI 415 CGGCCG C^GGCCG
993 CGGCCG
Eco52I 415 CGGCCG C^GGCCG
993 CGGCCG
HhaI 285 GCGC GCG^C
977 GCGC
HinP1I 285 GCGC G^CGC
977 GCGC
MaeI 532 CTAG C^TAG
1169 CTAG
MaeIII 26 GTTAC ^GT[ACGT]AC
527 GTGAC
MboI 825 GATC ^GATC
1033 GATC
NaeI 496 GCCGGC GCC^GGC
1658 GCCGGC
NdeII 825 GATC ^GATC
1033 GATC
NgoMIV 496 GCCGGC G^CCGGC
1658 GCCGGC
PstI 1209 CTGCAG CTGCA^G
1554 CTGCAG
Sau3AI 825 GATC ^GATC
1033 GATC
TfiI 667 GATTC G^A[AT]TC
1190 GATTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanI BanII BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CelII Cfr10I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI TspRI
TthHB8I Van91I