(Circular) (Six-base) MAPSORT of: pFN21AB5996.seq Check: 9981 from: 1 to: 6544 pFN21AB5996 6544 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3887 1937 Size: 1950 4594 Acc65I G'GTAC_C Cuts at: 3865 3865 Size: 6544 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2784 3116 4607 5991 6259 1632 Size: 1152 332 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 1152 332 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6347 AflIII A'CryG_T Cuts at: 1203 2156 3696 5443 6420 1203 Size: 953 1540 1747 977 1327 Fragments arranged by size: 1747 1540 1327 977 953 AgeI A'CCGG_T Cuts at: 5375 5375 Size: 6544 AhdI GACnn_n'nnGTC Cuts at: 5273 5273 Size: 6544 AleI CACnn'nnGTG Cuts at: 1166 3413 1166 Size: 2247 4297 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3108 1184 Size: 1924 4620 AlwNI CAG_nnn'CTG Cuts at: 2251 4287 5859 2251 Size: 2036 1572 2936 Fragments arranged by size: 2936 2036 1572 ApaI G_GGCC'C Cuts at: 3529 3529 Size: 6544 ApaLI G'TGCA_C Cuts at: 2070 4601 5757 2070 Size: 2531 1156 2857 Fragments arranged by size: 2857 2531 1156 ApoI r'AATT_y Cuts at: 1493 3853 4079 4231 6340 1493 Size: 2360 226 152 2109 1697 Fragments arranged by size: 2360 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2026 3869 1870 Size: 73 83 1843 4545 Fragments arranged by size: 4545 1843 83 73 AvrII C'CTAG_G Cuts at: 2536 2536 Size: 6544 BamHI G'GATC_C Cuts at: 3874 4241 3874 Size: 367 6177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 2199 2320 2353 2409 Size: 325 217 627 226 186 121 33 56 Cuts at: 2409 2540 2981 3865 5320 618 Size: 131 441 884 1455 1842 Fragments arranged by size: 1842 1455 884 627 441 325 226 217 186 131 121 56 33 BanII G_rGCy'C Cuts at: 1391 2516 2747 2895 3030 3056 3212 3309 3529 Size: 1125 231 148 135 26 156 97 220 Cuts at: 3529 3630 3687 3863 1391 Size: 101 57 176 4072 Fragments arranged by size: 4072 1125 231 220 176 156 148 135 101 97 57 26 BbeI G_GCGC'C Cuts at: 2985 2985 Size: 6544 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2312 3816 928 Size: 971 413 1504 3656 Fragments arranged by size: 3656 1504 971 413 BbvCI CC'TCA_GC Cuts at: 2205 2393 2205 Size: 188 6356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4735 4769 1903 Size: 34 2798 34 3678 Fragments arranged by size: 3678 2798 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4436 5646 4436 Size: 1210 5334 BclI T'GATC_A Cuts at: 1548 1548 Size: 6544 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2997 3185 3533 Size: 107 122 71 631 605 1325 188 348 Cuts at: 3533 5155 136 Size: 1622 1525 Fragments arranged by size: 1622 1525 1325 631 605 348 188 122 107 71 BglII A'GATC_T Cuts at: 3037 6539 3037 Size: 3502 3042 BlpI GC'TnA_GC Cuts at: 2167 3545 3671 3961 2167 Size: 1378 126 290 4750 Fragments arranged by size: 4750 1378 290 126 Bme1580I G_kGCm'C Cuts at: 1790 2074 2377 2545 3181 3529 4605 5761 1790 Size: 284 303 168 636 348 1076 1156 2573 Fragments arranged by size: 2573 1156 1076 636 348 303 284 168 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2339 4275 5233 Size: 416 53 83 399 24 922 1936 958 Cuts at: 5233 442 Size: 1753 Fragments arranged by size: 1936 1753 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6544 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2220 3049 3592 2220 Size: 829 543 5172 Fragments arranged by size: 5172 829 543 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2282 2449 2603 2633 2805 2873 3100 Size: 920 123 167 154 30 172 68 227 Cuts at: 3100 3530 5204 1239 Size: 430 1674 2579 Fragments arranged by size: 2579 1674 920 430 227 172 167 154 123 68 30 Bpu10I CC'TnA_GC Cuts at: 2205 2393 3419 2205 Size: 188 1026 5330 Fragments arranged by size: 5330 1026 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 2731 3188 4669 5534 5832 2579 Size: 152 457 1481 865 298 3291 Fragments arranged by size: 3291 1481 865 457 298 152 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3473 5207 882 Size: 617 1974 1734 2219 Fragments arranged by size: 2219 1974 1734 617 BsaAI yAC'GTr Cuts at: 493 1204 3415 4335 493 Size: 711 2211 920 2702 Fragments arranged by size: 2702 2211 920 711 BsaBI GATnn'nnATC Cuts at: 6538 6538 Size: 6544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2982 4351 4733 5368 Size: 53 83 186 936 1449 1369 382 635 Cuts at: 5368 275 Size: 1451 Fragments arranged by size: 1451 1449 1369 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3486 4977 5375 5649 5796 6207 3486 Size: 1491 398 274 147 411 3823 Fragments arranged by size: 3823 1491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2819 2849 1178 Size: 30 165 30 1416 30 4873 Fragments arranged by size: 4873 1416 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3168 3317 3527 1186 Size: 1982 149 210 4203 Fragments arranged by size: 4203 1982 210 149 BseYI C'CCAG_C Cuts at: 2315 2387 2677 2740 3324 3385 5747 6243 2315 Size: 72 290 63 584 61 2362 496 2616 Fragments arranged by size: 2616 2362 584 496 290 72 63 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2086 2099 2357 2416 3602 3691 1163 Size: 573 350 13 258 59 1186 89 4016 Fragments arranged by size: 4016 1186 573 350 258 89 59 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2423 3001 3994 4755 5783 1540 Size: 275 186 422 578 993 761 1028 2301 Fragments arranged by size: 2301 1028 993 761 578 422 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 3309 3420 4605 4690 5761 2074 Size: 1235 111 1185 85 1071 2857 Fragments arranged by size: 2857 1235 1185 1071 111 85 BsmI GAATG_Cn' Cuts at: 4055 4148 4055 Size: 93 6451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2377 2516 2545 2747 2895 3030 Size: 399 284 303 139 29 202 148 135 Cuts at: 3030 3056 3181 3212 3309 3420 3529 3630 3687 Size: 26 125 31 97 111 109 101 57 Cuts at: 3687 3863 4605 4690 5761 1391 Size: 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 284 202 176 148 139 135 125 111 109 101 97 85 57 31 29 26 BspEI T'CCGG_A Cuts at: 6207 6207 Size: 6544 BspHI T'CATG_A Cuts at: 4433 6163 4433 Size: 1730 4814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2874 3216 3899 844 Size: 2030 342 683 3489 Fragments arranged by size: 3489 2030 683 342 BsrBI CCG'CTC Cuts at: 1394 1823 2884 3636 4431 1394 Size: 429 1061 752 795 3507 Fragments arranged by size: 3507 1061 795 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2552 5039 5213 65 Size: 1183 1304 2487 174 1396 Fragments arranged by size: 2487 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2406 2501 3663 3710 5188 5375 1700 Size: 110 596 95 1162 47 1478 187 2869 Fragments arranged by size: 2869 1478 1162 596 187 110 95 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6544 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6544 BssSI C'ACGA_G Cuts at: 4604 5616 4604 Size: 1012 5532 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6544 BstBI TT'CG_AA Cuts at: 3363 5348 3363 Size: 1985 4559 BstEII G'GTnAC_C Cuts at: 1184 2030 1184 Size: 846 5698 BstXI CCAn_nnnn'nTGG Cuts at: 3022 3745 3022 Size: 723 5821 BstYI r'GATC_y Cuts at: 1972 3037 3874 4241 4626 4643 6095 6181 6193 Size: 1065 837 367 385 17 1452 86 12 Cuts at: 6193 6539 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1065 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2977 513 Size: 552 939 973 4080 Fragments arranged by size: 4080 973 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2882 487 Size: 683 56 1656 4149 Fragments arranged by size: 4149 1656 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2986 3556 3603 4060 4854 4874 2074 Size: 912 570 47 457 794 20 3744 Fragments arranged by size: 3744 912 794 570 457 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6509 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5551 809 Size: 507 4235 1802 Fragments arranged by size: 4235 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2037 2114 Size: 54 1425 50 275 18 177 30 77 Cuts at: 2114 2215 2420 2499 2998 3186 3991 4880 6509 Size: 101 205 79 499 188 805 889 1629 Cuts at: 6509 8 Size: 43 Fragments arranged by size: 1629 1425 889 805 499 275 205 188 177 101 79 77 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2420 2998 3991 1537 Size: 275 608 578 993 4090 Fragments arranged by size: 4090 993 608 578 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2936 4474 1353 Size: 1583 1538 3423 Fragments arranged by size: 3423 1583 1538 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5131 5505 5651 387 Size: 1064 138 3542 374 146 1280 Fragments arranged by size: 3542 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3307 3307 Size: 6544 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2282 2449 2603 2633 2784 2805 Size: 393 527 123 167 154 30 151 21 Cuts at: 2805 2873 3100 3116 3530 4607 5204 5991 6259 Size: 68 227 16 414 1077 597 787 268 Cuts at: 6259 1239 Size: 1524 Fragments arranged by size: 1524 1077 787 597 527 414 393 268 227 167 154 151 123 68 30 21 16 EcoNI CCTnn'n_nnAGG Cuts at: 3299 3299 Size: 6544 EcoO109I rG'GnC_Cy Cuts at: 2248 2341 3370 3424 3525 2248 Size: 93 1029 54 101 5267 Fragments arranged by size: 5267 1029 101 93 54 EcoRI G'AATT_C Cuts at: 3853 4231 3853 Size: 378 6166 FspI TGC'GCA Cuts at: 5050 5050 Size: 6544 HaeII r_GCGC'y Cuts at: 1678 2985 5691 1678 Size: 1307 2706 2531 Fragments arranged by size: 2706 2531 1307 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2819 2851 3186 3218 5185 Size: 32 135 32 1247 32 335 32 1967 Cuts at: 5185 5217 5259 5291 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1967 1247 335 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3888 4134 669 Size: 988 281 1950 246 3079 Fragments arranged by size: 3079 1950 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6544 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KasI G'GCGC_C Cuts at: 2981 2981 Size: 6544 KpnI G_GTAC'C Cuts at: 3869 3869 Size: 6544 MfeI C'AATT_G Cuts at: 4143 4143 Size: 6544 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 3312 5658 5842 1305 Size: 1197 810 2346 184 2007 Fragments arranged by size: 2346 2007 1197 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2217 3188 10 Size: 54 1425 343 177 208 971 3366 Fragments arranged by size: 3366 1425 971 343 208 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2155 3413 3713 4502 4861 5020 518 Size: 648 989 1258 300 789 359 159 2042 Fragments arranged by size: 2042 1258 989 789 648 359 300 159 MspA1I CmG'CkG Cuts at: 1606 2083 2611 2851 3247 3385 3960 4637 5785 Size: 477 528 240 396 138 575 677 1148 Cuts at: 5785 6030 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 575 528 477 396 245 240 138 NaeI GCC'GGC Cuts at: 2503 3665 2503 Size: 1162 5382 NarI GG'CG_CC Cuts at: 2982 2982 Size: 6544 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5053 Fragments arranged by size: 5053 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NgoMIV G'CCGG_C Cuts at: 2501 3663 2501 Size: 1162 5382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6544 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 2160 2381 3277 3700 3902 5447 1132 Size: 97 931 221 896 423 202 1545 2229 Fragments arranged by size: 2229 1545 931 896 423 221 202 97 PasI CC'CwG_GG Cuts at: 1348 3433 1348 Size: 2085 4459 PciI A'CATG_T Cuts at: 2156 3696 5443 2156 Size: 1540 1747 3257 Fragments arranged by size: 3257 1747 1540 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 2251 2363 2965 1108 Size: 1116 27 112 602 4687 Fragments arranged by size: 4687 1116 602 112 27 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 6544 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PmlI CAC'GTG Cuts at: 3415 3415 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 4613 4645 6126 6158 3782 Size: 32 799 32 1481 32 4168 Fragments arranged by size: 4168 1481 799 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2341 3370 3424 2248 Size: 93 1029 54 5368 Fragments arranged by size: 5368 1029 93 54 PshAI GACnn'nnGTC Cuts at: 1613 3000 1613 Size: 1387 5157 PsiI TTA'TAA Cuts at: 4114 4114 Size: 6544 PspOMI G'GGCC_C Cuts at: 3525 3525 Size: 6544 PstI C_TGCA'G Cuts at: 830 3218 3563 3896 830 Size: 2388 345 333 3478 Fragments arranged by size: 3478 2388 345 333 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 2083 2611 2851 3385 2083 Size: 528 240 534 5242 Fragments arranged by size: 5242 534 528 240 SacI G_AGCT'C Cuts at: 3309 3309 Size: 6544 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SbfI CC_TGCA'GG Cuts at: 3896 3896 Size: 6544 ScaI AGT'ACT Cuts at: 4792 6504 4792 Size: 1712 4832 SfcI C'TryA_G Cuts at: 826 1045 2606 3214 3559 3892 5027 5708 5899 Size: 219 1561 608 345 333 1135 681 191 Cuts at: 5899 826 Size: 1471 Fragments arranged by size: 1561 1471 1135 681 608 345 333 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3533 3533 Size: 6544 SfoI GGC'GCC Cuts at: 2983 2983 Size: 6544 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 3871 1872 Size: 1999 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 2746 3203 4648 5549 5811 Size: 197 926 651 152 457 1445 901 262 Cuts at: 5811 820 Size: 1553 Fragments arranged by size: 1553 1445 926 901 651 457 262 197 152 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3902 1132 Size: 2770 3774 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 2631 2782 3536 1517 Size: 1114 151 754 4525 Fragments arranged by size: 4525 1114 754 151 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 2545 3339 3738 3983 513 Size: 552 939 532 9 794 399 245 3074 Fragments arranged by size: 3074 939 794 552 532 399 245 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2160 3425 3614 4571 4588 4741 4926 Size: 1255 580 1265 189 957 17 153 185 Cuts at: 4926 5371 325 Size: 445 1498 Fragments arranged by size: 1498 1265 1255 957 580 445 189 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4790 6502 96 Size: 274 80 33 51 1443 2813 1712 138 Fragments arranged by size: 2813 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2135 2167 6504 6536 2135 Size: 32 4337 32 2143 Fragments arranged by size: 4337 2143 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6544 XbaI T'CTAG_A Cuts at: 3880 3880 Size: 6544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2095 2095 Size: 6544 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6544 XmaI C'CCGG_G Cuts at: 1870 3869 1870 Size: 1999 4545 XmnI GAAnn'nnTTC Cuts at: 4673 4673 Size: 6544 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI