Restriction map for
pFN21AB5996
(Linear) (Six-base) MAPSORT of: KIBB5996.seq Check: 4058 from: 1 to: 1839
KIBB5996.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1111 1839
Size: 779 332 728
Fragments arranged by size:
779 728 332
AflIII A'CryG_T
Cuts at: 0 151 1691 1839
Size: 151 1540 148
Fragments arranged by size:
1540 151 148
AleI CACnn'nnGTG
Cuts at: 0 1408 1839
Size: 1408 431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1103 1839
Size: 1103 736
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1839
Size: 246 1593
ApaI G_GGCC'C
Cuts at: 0 1524 1839
Size: 1524 315
ApaLI G'TGCA_C
Cuts at: 0 65 1839
Size: 65 1774
AvaI C'yCGr_G
Cuts at: 0 21 1839
Size: 21 1818
AvrII C'CTAG_G
Cuts at: 0 531 1839
Size: 531 1308
BanI G'GyrC_C
Cuts at: 0 8 194 315 348 404 535 976 1839
Size: 8 186 121 33 56 131 441 863
Fragments arranged by size:
863 441 186 131 121 56 33 8
BanII G_rGCy'C
Cuts at: 0 511 742 890 1025 1051 1207 1304 1524
Size: 511 231 148 135 26 156 97 220
Cuts at: 1524 1625 1682 1839
Size: 101 57 157
Fragments arranged by size:
511 231 220 157 156 148 135 101
97 57 26
BbeI G_GCGC'C
Cuts at: 0 980 1839
Size: 980 859
BbsI GAAGACnn'nnnn_
Cuts at: 0 307 1811 1839
Size: 307 1504 28
Fragments arranged by size:
1504 307 28
BbvCI CC'TCA_GC
Cuts at: 0 200 388 1839
Size: 200 188 1451
Fragments arranged by size:
1451 200 188
BglI GCCn_nnn'nGGC
Cuts at: 0 992 1180 1528 1839
Size: 992 188 348 311
Fragments arranged by size:
992 348 311 188
BglII A'GATC_T
Cuts at: 0 1032 1839
Size: 1032 807
BlpI GC'TnA_GC
Cuts at: 0 162 1540 1666 1839
Size: 162 1378 126 173
Fragments arranged by size:
1378 173 162 126
Bme1580I G_kGCm'C
Cuts at: 0 69 372 540 1176 1524 1839
Size: 69 303 168 636 348 315
Fragments arranged by size:
636 348 315 303 168 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 1839
Size: 334 1505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 215 1044 1587 1839
Size: 215 829 543 252
Fragments arranged by size:
829 543 252 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 277 444 598 628 800 868 1095
Size: 154 123 167 154 30 172 68 227
Cuts at: 1095 1525 1839
Size: 430 314
Fragments arranged by size:
430 314 227 172 167 154 154 123
68 30
Bpu10I CC'TnA_GC
Cuts at: 0 200 388 1414 1839
Size: 200 188 1026 425
Fragments arranged by size:
1026 425 200 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 726 1183 1839
Size: 574 152 457 656
Fragments arranged by size:
656 574 457 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 1468 1839
Size: 1468 371
BsaAI yAC'GTr
Cuts at: 0 1410 1839
Size: 1410 429
BsaHI Gr'CG_yC
Cuts at: 0 977 1839
Size: 977 862
BsaWI w'CCGG_w
Cuts at: 0 1481 1839
Size: 1481 358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 814 844 1839
Size: 814 30 995
Fragments arranged by size:
995 814 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1163 1312 1522 1839
Size: 1163 149 210 317
Fragments arranged by size:
1163 317 210 149
BseYI C'CCAG_C
Cuts at: 0 310 382 672 735 1319 1380 1839
Size: 310 72 290 63 584 61 459
Fragments arranged by size:
584 459 310 290 72 63 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 94 352 411 1597 1686 1839
Size: 81 13 258 59 1186 89 153
Fragments arranged by size:
1186 258 153 89 81 59 13
BsiEI CG_ry'CG
Cuts at: 0 418 996 1839
Size: 418 578 843
Fragments arranged by size:
843 578 418
BsiHKAI G_wGCw'C
Cuts at: 0 69 1304 1415 1839
Size: 69 1235 111 424
Fragments arranged by size:
1235 424 111 69
Bsp1286I G_dGCh'C
Cuts at: 0 69 372 511 540 742 890 1025 1051
Size: 69 303 139 29 202 148 135 26
Cuts at: 1051 1176 1207 1304 1415 1524 1625 1682 1839
Size: 125 31 97 111 109 101 57 157
Fragments arranged by size:
303 202 157 148 139 135 125 111
109 101 97 69 57 31 29 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 869 1211 1839
Size: 869 342 628
Fragments arranged by size:
869 628 342
BsrBI CCG'CTC
Cuts at: 0 879 1631 1839
Size: 879 752 208
Fragments arranged by size:
879 752 208
BsrDI GCAATG_nn'
Cuts at: 0 547 1839
Size: 547 1292
BsrFI r'CCGG_y
Cuts at: 0 401 496 1658 1705 1839
Size: 401 95 1162 47 134
Fragments arranged by size:
1162 401 134 95 47
BstBI TT'CG_AA
Cuts at: 0 1358 1839
Size: 1358 481
BstEII G'GTnAC_C
Cuts at: 0 25 1839
Size: 25 1814
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1017 1740 1839
Size: 1017 723 99
Fragments arranged by size:
1017 723 99
BstYI r'GATC_y
Cuts at: 0 1032 1839
Size: 1032 807
BtgI C'CryG_G
Cuts at: 0 972 1839
Size: 972 867
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 877 1839
Size: 877 962
BtsI GCAGTG_nn'
Cuts at: 0 69 981 1551 1598 1839
Size: 69 912 570 47 241
Fragments arranged by size:
912 570 241 69 47
EaeI y'GGCC_r
Cuts at: 0 2 32 109 210 415 494 993 1181
Size: 2 30 77 101 205 79 499 188
Cuts at: 1181 1839
Size: 658
Fragments arranged by size:
658 499 205 188 101 79 77 30
2
EagI C'GGCC_G
Cuts at: 0 415 993 1839
Size: 415 578 846
Fragments arranged by size:
846 578 415
EarI CTCTTCn'nnn_
Cuts at: 0 931 1839
Size: 931 908
EcoICRI GAG'CTC
Cuts at: 0 1302 1839
Size: 1302 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 277 444 598 628 779 800 868
Size: 154 123 167 154 30 151 21 68
Cuts at: 868 1095 1111 1525 1839
Size: 227 16 414 314
Fragments arranged by size:
414 314 227 167 154 154 151 123
68 30 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1839
Size: 1294 545
EcoO109I rG'GnC_Cy
Cuts at: 0 243 336 1365 1419 1520 1839
Size: 243 93 1029 54 101 319
Fragments arranged by size:
1029 319 243 101 93 54
HaeII r_GCGC'y
Cuts at: 0 980 1839
Size: 980 859
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 1181 1213 1839
Size: 814 32 335 32 626
Fragments arranged by size:
814 626 335 32 32
KasI G'GCGC_C
Cuts at: 0 976 1839
Size: 976 863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1307 1839
Size: 497 810 532
Fragments arranged by size:
810 532 497
MscI TGG'CCA
Cuts at: 0 4 212 1183 1839
Size: 4 208 971 656
Fragments arranged by size:
971 656 208 4
MslI CAynn'nnrTG
Cuts at: 0 150 1408 1708 1839
Size: 150 1258 300 131
Fragments arranged by size:
1258 300 150 131
MspA1I CmG'CkG
Cuts at: 0 78 606 846 1242 1380 1839
Size: 78 528 240 396 138 459
Fragments arranged by size:
528 459 396 240 138 78
NaeI GCC'GGC
Cuts at: 0 498 1660 1839
Size: 498 1162 179
Fragments arranged by size:
1162 498 179
NarI GG'CG_CC
Cuts at: 0 977 1839
Size: 977 862
NgoMIV G'CCGG_C
Cuts at: 0 496 1658 1839
Size: 496 1162 181
Fragments arranged by size:
1162 496 181
NspI r_CATG'y
Cuts at: 0 155 376 1272 1695 1839
Size: 155 221 896 423 144
Fragments arranged by size:
896 423 221 155 144
PasI CC'CwG_GG
Cuts at: 0 1428 1839
Size: 1428 411
PciI A'CATG_T
Cuts at: 0 151 1691 1839
Size: 151 1540 148
Fragments arranged by size:
1540 151 148
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 246 358 960 1839
Size: 219 27 112 602 879
Fragments arranged by size:
879 602 219 112 27
PfoI T'CCnGG_A
Cuts at: 0 245 1839
Size: 245 1594
PmlI CAC'GTG
Cuts at: 0 1410 1839
Size: 1410 429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1777 1809 1839
Size: 1777 32 30
Fragments arranged by size:
1777 32 30
PpuMI rG'GwC_Cy
Cuts at: 0 243 336 1365 1419 1839
Size: 243 93 1029 54 420
Fragments arranged by size:
1029 420 243 93 54
PshAI GACnn'nnGTC
Cuts at: 0 995 1839
Size: 995 844
PspOMI G'GGCC_C
Cuts at: 0 1520 1839
Size: 1520 319
PstI C_TGCA'G
Cuts at: 0 1213 1558 1839
Size: 1213 345 281
Fragments arranged by size:
1213 345 281
PvuII CAG'CTG
Cuts at: 0 78 606 846 1380 1839
Size: 78 528 240 534 459
Fragments arranged by size:
534 528 459 240 78
SacI G_AGCT'C
Cuts at: 0 1304 1839
Size: 1304 535
SfcI C'TryA_G
Cuts at: 0 601 1209 1554 1839
Size: 601 608 345 285
Fragments arranged by size:
608 601 345 285
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1528 1839
Size: 1528 311
SfoI GGC'GCC
Cuts at: 0 978 1839
Size: 978 861
SmlI C'TyrA_G
Cuts at: 0 589 741 1198 1839
Size: 589 152 457 641
Fragments arranged by size:
641 589 457 152
StuI AGG'CCT
Cuts at: 0 626 777 1531 1839
Size: 626 151 754 308
Fragments arranged by size:
754 626 308 151
StyI C'CwwG_G
Cuts at: 0 531 540 1334 1733 1839
Size: 531 9 794 399 106
Fragments arranged by size:
794 531 399 106 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 155 1420 1609 1839
Size: 155 1265 189 230
Fragments arranged by size:
1265 230 189 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 130 162 1839
Size: 130 32 1677
Fragments arranged by size:
1677 130 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 1839
Size: 90 1749
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SfcI SfiI SfoI SmlI StuI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApoI AscI AseI AsiSI BaeI BamHI
BcgI BciVI BclI BfrBI BmgBI BmtI BsaBI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI