Restriction map for
pFN21AB6352
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BbvCI BcgI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BseRI BsgI BsiEI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII CpoI
DraI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcyI 620 GGCGCC G[GA]^CG[CT]C
AlwNI 209 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 46 GAGCTC G[AT]GC[AT]^C
AvaI 491 CCCGGG C^[CT]CG[GA]G
AviII 341 TGCGCA TGC^GCA
BalI 464 TGGCCA TGG^CCA
BamHI 524 GGATCC G^GATCC
BanI 620 GGCGCC G^G[CT][GA]CC
BanII 46 GAGCTC G[GA]GC[CT]^C
BbeI 620 GGCGCC GGCGC^C
BciVI 711 GTATCC GTATCC(6/5)
BclI 59 TGATCA T^GATCA
BmyI 46 GAGCTC G[AGT]GC[ACT]^C
BpmI 473 CTGGAG CTGGAG(16/14)
BsaHI 620 GGCGCC G[GA]^CG[CT]C
BsaI 304 GGTCTC GGTCTC(1/5)
BsaWI 164 ACCGGA [AT]^CCGG[AT]
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
BsmBI 661 GAGACG CGTCTC(1/5)
BsmFI 512 GGGAC GGGAC(10/14)
BsoBI 491 CCCGGG C^[CT]CG[GA]G
Bsp1286I 46 GAGCTC G[AGT]GC[ACT]^C
BspDI 583 ATCGAT AT^CGAT
BspMI 724 ACCTGC ACCTGC(4/8)
BsrBI 127 GAGCGG CCGCTC(-3/-3)
BsrDI 27 CATTGC GCAATG(2/0)
BsrI 642 CCAGT ACTGG(1/-1)
BstAPI 693 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 524 GGATCC [GA]^GATC[CT]
ClaI 583 ATCGAT AT^CGAT
DrdI 775 GACTACTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 503 CCGTGG C^C[GA][CT]GG
EcoT14I 197 CCAAGG C^C[AT][AT]GG
FbaI 59 TGATCA T^GATCA
FspI 341 TGCGCA TGC^GCA
HaeII 620 GGCGCC [GA]GCGC^[CT]
Hin1I 620 GGCGCC G[GA]^CG[CT]C
KasI 620 GGCGCC G^GCGCC
MflI 524 GGATCC [GA]^GATC[CT]
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
NaeI 617 GCCGGC GCC^GGC
NarI 620 GGCGCC GG^CGCC
NgoMIV 617 GCCGGC G^CCGGC
PleI 316 GACTC GAGTC(4/5)
PstI 367 CTGCAG CTGCA^G
PvuII 55 CAGCTG CAG^CTG
SacI 46 GAGCTC GAGCT^C
SapI 76 GAAGAGC GCTCTTC(1/4)
SfcI 367 CTGCAG C^T[GA][CT]AG
SfoI 620 GGCGCC GGC^GCC
SmaI 491 CCCGGG CCC^GGG
StuI 674 AGGCCT AGG^CCT
StyI 197 CCAAGG C^C[AT][AT]GG
TaqI 584 TCGA T^CGA
TfiI 19 GATTC G^A[AT]TC
Tsp45I 413 GTGAC ^GT[GC]AC
TthHB8I 584 TCGA T^CGA
XhoII 524 GGATCC [GA]^GATC[CT]
XmaCI 491 CCCGGG C^CCGGG
XmaI 491 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 449 CCTCAGG CC^T[ACGT]AGG
670 CCTGAGG
AvaII 666 GGACC G^G[AT]CC
783 GGTCC
BsaAI 460 TACGTG [CT]AC^GT[GA]
847 TACGTG
BsrFI 301 GCCGGT [GA]^CCGG[CT]
617 GCCGGC
BssSI 7 CTCGTG CACGAG(-5/-1)
697 CTCGTG
Bsu36I 449 CCTCAGG CC^T[ACGT]AGG
670 CCTGAGG
Cac8I 617 GCCGGC GC[ACGT]^[ACGT]GC
702 GCTGGC
Cfr10I 301 GCCGGT [GA]^CCGG[CT]
617 GCCGGC
EarI 76 GAAGAG CTCTTC(1/4)
274 GAAGAG
Eco81I 449 CCTCAGG CC^T[ACGT]AGG
670 CCTGAGG
EcoNI 445 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
528 CCTGGCCGAGG
FauI 23 CCCGC CCCGC(4/6)
633 CCCGC
HinfI 19 GATTC G^A[ACGT]TC
316 GACTC
HphI 412 GGTGA GGTGA(8/7)
495 GGTGA
Ksp632I 76 GAAGAG CTCTTC(1/4)
274 GAAGAG
MaeII 461 ACGT A^CGT
848 ACGT
MaeIII 413 GTGAC ^GT[ACGT]AC
545 GTTAC
SfaNI 132 GATGC GCATC(5/9)
205 GATGC
TspRI 520 CAGTG CA[GC]TG[ACGT][ACGT]^
643 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AlwI BbsI
BbvI BcnI BpuAI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
BstUI CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI