Restriction map for
pFN21AB6352
(Circular) (Six-base) MAPSORT of: pFN21AB6352.seq Check: 8382 from: 1 to: 5584
pFN21AB6352 5584 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3647 5031 5299 1632
Size: 2015 1384 268 1917
Fragments arranged by size:
2015 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 1184
Size: 1188 4396
AlwNI CAG_nnn'CTG
Cuts at: 2219 3327 4899 2219
Size: 1108 1572 2904
Fragments arranged by size:
2904 1572 1108
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2496 2909 1870
Size: 73 553 413 4545
Fragments arranged by size:
4545 553 413 73
BamHI G'GATC_C
Cuts at: 2529 2914 3281 2529
Size: 385 367 4832
Fragments arranged by size:
4832 385 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2625 2905 4360 618
Size: 325 217 627 838 280 1455 1842
Fragments arranged by size:
1842 1455 838 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2055 2903 1391
Size: 664 848 4072
Fragments arranged by size:
4072 848 664
BbeI G_GCGC'C
Cuts at: 2629 2629
Size: 5584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2129 2345 2805 928
Size: 971 230 216 460 3707
Fragments arranged by size:
3707 971 460 230 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3476 4686 2727
Size: 749 1210 3625
Fragments arranged by size:
3625 1210 749
BclI T'GATC_A
Cuts at: 1548 2064 1548
Size: 516 5068
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 3645 4801 1790
Size: 1855 1156 2573
Fragments arranged by size:
2573 1855 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2641 3315 4273
Size: 416 53 83 399 24 1224 674 958
Cuts at: 4273 442
Size: 1753
Fragments arranged by size:
1753 1224 958 674 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2499 4244 1239
Size: 1260 1745 2579
Fragments arranged by size:
2579 1745 1260
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 4247 882
Size: 617 816 1932 2219
Fragments arranged by size:
2219 1932 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 2854 3375 493
Size: 711 1263 387 521 2702
Fragments arranged by size:
2702 1263 711 521 387
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2626 3391 3773 4408
Size: 53 83 186 936 1093 765 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1093 936 765 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2169 4017 4415 4689 4836 5247 2169
Size: 1848 398 274 147 411 2506
Fragments arranged by size:
2506 1848 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2472 2481 2502 1178
Size: 30 165 30 1048 21 9 21 4260
Fragments arranged by size:
4260 1048 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5584
BseYI C'CCAG_C
Cuts at: 4787 5283 4787
Size: 496 5088
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5011
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 3645 3730 4801 2055
Size: 1590 85 1071 2838
Fragments arranged by size:
2838 1590 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
BsmBI CGTCTCn'nnnn_
Cuts at: 2660 2660
Size: 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2903 3645 3730 4801 1391
Size: 399 265 848 742 85 1071 2174
Fragments arranged by size:
2174 1071 848 742 399 265 85
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 2939 844
Size: 1894 201 3489
Fragments arranged by size:
3489 1894 201
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 3471 1394
Size: 429 311 1337 3507
Fragments arranged by size:
3507 1337 429 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 2031 4079 4253 65
Size: 1183 783 2048 174 1396
Fragments arranged by size:
2048 1396 1183 783 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2306 2622 4228 4415 1700
Size: 110 496 316 1606 187 2869
Fragments arranged by size:
2869 1606 496 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 2012 2702 3644 4656 2012
Size: 690 942 1012 2940
Fragments arranged by size:
2940 1012 942 690
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 794
Size: 1910 3674
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2529 2914 3281 3666 3683 5135 5221 5233
Size: 557 385 367 385 17 1452 86 12
Cuts at: 5233 5579 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 557 385 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2455 2676 2455
Size: 221 5363
BtgI C'CryG_G
Cuts at: 513 1065 2004 2508 513
Size: 552 939 504 3589
Fragments arranged by size:
3589 939 552 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2531 3100 3894 3914 2531
Size: 569 794 20 4201
Fragments arranged by size:
4201 794 569 20
ClaI AT'CG_AT
Cuts at: 1554 2589 1554
Size: 1035 4549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2786 4591 809
Size: 507 1470 1805 1802
Fragments arranged by size:
1805 1802 1470 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2382 2469
Size: 54 1425 50 275 18 465 87 87
Cuts at: 2469 2535 3031 3920 5549 8
Size: 66 496 889 1629 43
Fragments arranged by size:
1629 1425 889 496 465 275 87 87
66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2274 3514 1353
Size: 723 198 1240 3423
Fragments arranged by size:
3423 1240 723 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 4171 4545 4691 387
Size: 1064 138 713 1869 374 146 1280
Fragments arranged by size:
1869 1280 1064 713 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2499 3647 4244 5031 5299 1239
Size: 393 867 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 867 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2454 2537 2454
Size: 83 5501
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 2348 4090 2348
Size: 1742 3842
HaeII r_GCGC'y
Cuts at: 1678 2629 4731 1678
Size: 951 2102 2531
Fragments arranged by size:
2531 2102 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2768 2800 4225
Size: 32 135 32 721 32 443 32 1425
Cuts at: 4225 4257 4299 4331 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 721 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KasI G'GCGC_C
Cuts at: 2625 2625
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 4698 4882 1305
Size: 951 2442 184 2007
Fragments arranged by size:
2442 2007 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 10
Size: 54 1425 343 639 3123
Fragments arranged by size:
3123 1425 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 3901 4060 518
Size: 648 2376 359 159 2042
Fragments arranged by size:
2376 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2641 2767 3000 3677 4825 5070 1606
Size: 456 579 126 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 456 245 233 126
NaeI GCC'GGC
Cuts at: 2624 2624
Size: 5584
NarI GG'CG_CC
Cuts at: 2626 2626
Size: 5584
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NgoMIV G'CCGG_C
Cuts at: 2622 2622
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 3653 3685 5166 5198 2669
Size: 32 952 32 1481 32 3055
Fragments arranged by size:
3055 1481 952 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2376 2936 830
Size: 1546 560 3478
Fragments arranged by size:
3478 1546 560
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5584
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SapI GCTCTTCn'nnn_
Cuts at: 2076 2076
Size: 5584
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2372 2932 4067 4748 4939 826
Size: 219 1327 560 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 681 560 219 191
SfoI GGC'GCC
Cuts at: 2627 2627
Size: 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2498 2911 1872
Size: 626 413 4545
Fragments arranged by size:
4545 626 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 2681 1517
Size: 1164 4420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 3023 513
Size: 552 939 198 821 3074
Fragments arranged by size:
3074 939 821 552 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3611 3628 3781 3966 4411 325
Size: 1255 1196 835 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1196 835 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2401 2433 2451 2472 2483 2504 2510 2542 5544
Size: 32 18 21 11 21 6 32 3002
Cuts at: 5544 5576 2401
Size: 32 2409
Fragments arranged by size:
3002 2409 32 32 32 21 21 18
11 6
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2496 2909 1870
Size: 626 413 4545
Fragments arranged by size:
4545 626 413
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI