################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AccII AclI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BciVI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BsoBI BspDI BspLU11I BssHII Bst1107I BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 601 GACGTC GACGT^C AccI 598 GTAGAC GT^[AC][GT]AC AccIII 2453 TCCGGA T^CCGGA AcyI 601 GACGTC G[GA]^CG[CT]C AflII 826 CTTAAG C^TTAAG AflIII 534 ACGTGT A^C[GA][CT]GT AgeI 2279 ACCGGT A^CCGGT Alw44I 2724 GTGCAC G^TGCAC ApaLI 2724 GTGCAC G^TGCAC BanII 2716 GAGCTC G[GA]GC[CT]^C BbvCI 882 GCTGAGG CCTCAGC(-5/-2) BcgI 2041 CGAGTAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1956 TGATCA T^GATCA BfrI 826 CTTAAG C^TTAAG BsaBI 1129 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 601 GACGTC G[GA]^CG[CT]C BseAI 2453 TCCGGA T^CCGGA Bsp1407I 335 TGTACA T^GTACA BspEI 2453 TCCGGA T^CCGGA BspHI 2361 TCATGA T^CATGA BspMI 2005 GCAGGT ACCTGC(4/8) BsrBI 832 GAGCGG CCGCTC(-3/-3) BsrFI 2279 ACCGGT [GA]^CCGG[CT] BsrGI 335 TGTACA T^GTACA BstBI 271 TTCGAA TT^CGAA BstXI 715 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 2279 ACCGGT [GA]^CCGG[CT] DraIII 2225 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2 TGGCCG [CT]^GGCC[GA] EcoT22I 1555 ATGCAT ATGCA^T FbaI 1956 TGATCA T^GATCA Hin1I 601 GACGTC G[GA]^CG[CT]C MamI 1129 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2453 TCCGGA T^CCGGA NsiI 1555 ATGCAT ATGCA^T NspI 764 GCATGC [GA]CATG^[CT] NspV 271 TTCGAA TT^CGAA PinAI 2279 ACCGGT A^CCGGT RcaI 2361 TCATGA T^CATGA SacI 2716 GAGCTC GAGCT^C SfuI 271 TTCGAA TT^CGAA SmlI 826 CTTAAG C^T[CT][GA]AG SpeI 2208 ACTAGT A^CTAGT SphI 764 GCATGC GCATG^C SspBI 335 TGTACA T^GTACA SspI 2132 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 1010 CCTCAGG CC^T[ACGT]AGG 2500 CCTCAGG Asp700I 1063 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2593 GAATCTCTTC BamHI 777 GGATCC G^GATCC 966 GGATCC BanI 465 GGCACC G^G[CT][GA]CC 558 GGCACC BsaI 2446 GAGACC GGTCTC(1/5) 2008 GGTCTC BsaWI 2279 ACCGGT [AT]^CCGG[AT] 2453 TCCGGA BseRI 1008 CTCCTC GAGGAG(10/8) 1382 CTCCTC BsgI 2558 CTGCAC GTGCAG(16/14) 917 GTGCAG BsrDI 2052 CATTGC GCAATG(2/0) 2553 CATTGC BstAPI 1656 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2527 GCAGAGTTTGC Bsu36I 1010 CCTCAGG CC^T[ACGT]AGG 2500 CCTCAGG CfoI 1075 GCGC GCG^C 2222 GCGC DsaI 820 CCATGG C^C[GA][CT]GG 2057 CCATGG Eco81I 1010 CCTCAGG CC^T[ACGT]AGG 2500 CCTCAGG HapII 2280 CCGG C^CGG 2454 CCGG HhaI 1075 GCGC GCG^C 2222 GCGC HinP1I 1075 GCGC G^CGC 2222 GCGC HpaII 2280 CCGG C^CGG 2454 CCGG MspI 2280 CCGG C^CGG 2454 CCGG NcoI 820 CCATGG C^CATGG 2057 CCATGG PflMI 1638 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1768 CCAGCTAATGG ScaI 921 AGTACT AGT^ACT 2396 AGTACT Van91I 1638 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1768 CCAGCTAATGG XcmI 1226 CCAAAGAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1457 CCAGAGTTCGGCTGG XmnI 1063 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2593 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII