Restriction map for
pFN21AF0120
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# NO CUT ENZYMES ( 55 enzymes) #
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AatII AclI AflII AocI AscI
AseI AsnI Asp700I AspI AviII
AvrII BamHI BciVI BfrI BlnI
BsiWI BsrDI Bst1107I BstZ17I Bsu36I
CpoI DraI DraIII EagI EclXI
Eco52I Eco81I EcoRI FseI FspI
HpaI KspI MfeI MunI NdeI
NheI NotI PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SacII
SexAI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 2270 GGTACC G^GTACC
AccIII 2603 TCCGGA T^CCGGA
AcsI 286 GAATTT [GA]^AATT[CT]
AgeI 701 ACCGGT A^CCGGT
AhdI 1404 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2628 AGCGCT AGC^GCT
ApoI 286 GAATTT [GA]^AATT[CT]
Asp718I 2270 GGTACC G^GTACC
AspEI 1404 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1307 GGCGCC GGCGC^C
BbrPI 1972 CACGTG CAC^GTG
BbvCI 2871 CCTCAGC CCTCAGC(-5/-2)
BcgI 1751 GCAGCACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1797 AGATCT A^GATCT
BlpI 96 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 96 GCTCAGC GC^T[ACGT]AGC
BsaAI 1972 CACGTG [CT]AC^GT[GA]
BsaBI 1248 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2603 TCCGGA T^CCGGA
BsiEI 921 CGACCG CG[GA][CT]^CG
BsmI 3103 GAATGC GAATGC(1/-1)
BspDI 685 ATCGAT AT^CGAT
BspEI 2603 TCCGGA T^CCGGA
BspHI 656 TCATGA T^CATGA
BspLU11I 3368 ACATGT A^CATGT
BssHII 2447 GCGCGC G^CGCGC
BstBI 3100 TTCGAA TT^CGAA
BstEII 1956 GGTCACC G^GT[ACGT]ACC
BstPI 1956 GGTCACC G^GT[ACGT]ACC
CelII 96 GCTCAGC GC^T[ACGT]AGC
ClaI 685 ATCGAT AT^CGAT
Eam1105I 1404 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2628 AGCGCT AGC^GCT
EcoO65I 1956 GGTCACC G^GT[ACGT]ACC
EcoRV 3019 GATATC GAT^ATC
EcoT22I 3105 ATGCAT ATGCA^T
HindIII 2676 AAGCTT A^AGCTT
KasI 1307 GGCGCC G^GCGCC
KpnI 2270 GGTACC GGTAC^C
MamI 1248 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3095 ACGCGT A^CGCGT
MroI 2603 TCCGGA T^CCGGA
NarI 1307 GGCGCC GG^CGCC
NcoI 2993 CCATGG C^CATGG
NruI 2668 TCGCGA TCG^CGA
NsiI 3105 ATGCAT ATGCA^T
NspV 3100 TTCGAA TT^CGAA
PaeR7I 679 CTCGAG C^TCGAG
PflMI 2472 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 701 ACCGGT A^CCGGT
PmaCI 1972 CACGTG CAC^GTG
PmlI 1972 CACGTG CAC^GTG
PshAI 1336 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 656 TCATGA T^CATGA
SacI 1042 GAGCTC GAGCT^C
SalI 2455 GTCGAC G^TCGAC
SbfI 975 CCTGCAGG CCTGCA^GG
SfiI 367 GGCCACGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1307 GGCGCC GGC^GCC
SfuI 3100 TTCGAA TT^CGAA
Sse8387I 975 CCTGCAGG CCTGCA^GG
Tsp509I 287 AATT ^AATT
Van91I 2472 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 347 CCACCATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 679 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 2455 GTCGAC GT^[AC][GT]AC
2572 GTAGAC
AflIII 3095 ACGCGT A^C[GA][CT]GT
3368 ACATGT
Alw44I 2074 GTGCAC G^TGCAC
2401 GTGCAC
ApaI 1980 GGGCCC GGGCC^C
1987 GGGCCC
ApaLI 2074 GTGCAC G^TGCAC
2401 GTGCAC
BclI 2306 TGATCA T^GATCA
3191 TGATCA
BglI 368 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
988 GCCCGCCAGGC
BsaI 668 GAGACC GGTCTC(1/5)
3061 GAGACC
Bsp1407I 305 TGTACA T^GTACA
1079 TGTACA
BsrGI 305 TGTACA T^GTACA
1079 TGTACA
BstAPI 2967 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3150 GCACTGCCTGC
DsaI 2993 CCATGG C^C[GA][CT]GG
3353 CCACGG
FbaI 2306 TGATCA T^GATCA
3191 TGATCA
MaeII 1973 ACGT A^CGT
2405 ACGT
MseI 182 TTAA T^TAA
1189 TTAA
PpuMI 434 AGGACCT [GA]G^G[AT]CC[CT]
1034 AGGACCT
ScaI 1175 AGTACT AGT^ACT
1463 AGTACT
SmaI 1701 CCCGGG CCC^GGG
2648 CCCGGG
SmlI 679 CTCGAG C^T[CT][GA]AG
1045 CTCAAG
SspBI 305 TGTACA T^GTACA
1079 TGTACA
StuI 1142 AGGCCT AGG^CCT
1274 AGGCCT
TfiI 341 GATTC G^A[AT]TC
1426 GATTC
Tru9I 182 TTAA T^TAA
1189 TTAA
XmaCI 1701 CCCGGG C^CCGGG
2648 CCCGGG
XmaI 1701 CCCGGG C^CCGGG
2648 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII