(Circular) (Six-base) MAPSORT of: pFN21AF0120.seq Check: 8063 from: 1 to: 8167 pFN21AF0120 8167 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5977 278 Size: 53 83 186 936 4441 2468 Fragments arranged by size: 4441 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4461 4578 5510 1937 Size: 2524 117 932 4594 Fragments arranged by size: 4594 2524 932 117 Acc65I G'GTAC_C Cuts at: 4275 5488 4275 Size: 1213 6954 AclI AA'CG_TT Cuts at: 1563 6294 6667 1563 Size: 4731 373 3063 Fragments arranged by size: 4731 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2783 2852 3477 3530 3572 4409 4688 Size: 437 714 69 625 53 42 837 279 Cuts at: 4688 5339 6230 7614 7882 1632 Size: 651 891 1384 268 1917 Fragments arranged by size: 1917 1384 891 837 714 651 625 437 279 268 69 53 42 AfeI AGC'GCT Cuts at: 4635 4635 Size: 8167 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7970 AflIII A'CryG_T Cuts at: 1203 5100 5373 7066 8043 1203 Size: 3897 273 1693 977 1327 Fragments arranged by size: 3897 1693 1327 977 273 AgeI A'CCGG_T Cuts at: 2706 6998 2706 Size: 4292 3875 AhdI GACnn_n'nnGTC Cuts at: 3414 6896 3414 Size: 3482 4685 AleI CACnn'nnGTG Cuts at: 1166 2357 3968 4413 1166 Size: 1191 1611 445 4920 Fragments arranged by size: 4920 1611 1191 445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3539 1184 Size: 2355 5812 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5282 5314 5282 Size: 32 8135 AlwNI CAG_nnn'CTG Cuts at: 2803 2851 2959 3349 4141 4549 4978 5910 7482 Size: 48 108 390 792 408 429 932 1572 Cuts at: 7482 2803 Size: 3488 Fragments arranged by size: 3488 1572 932 792 429 408 390 108 48 ApaI G_GGCC'C Cuts at: 3989 3996 3989 Size: 7 8160 ApaLI G'TGCA_C Cuts at: 4079 4406 6224 7380 4079 Size: 327 1818 1156 4866 Fragments arranged by size: 4866 1818 1156 327 ApoI r'AATT_y Cuts at: 1493 2291 5476 5702 5854 7963 1493 Size: 798 3185 226 152 2109 1697 Fragments arranged by size: 3185 2109 1697 798 226 152 AseI AT'TA_AT Cuts at: 160 1032 6721 160 Size: 872 5689 1606 Fragments arranged by size: 5689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 AvaI C'yCGr_G Cuts at: 1870 1943 2684 3159 3706 4653 4771 4987 5082 Size: 73 741 475 547 947 118 216 95 Cuts at: 5082 5492 1870 Size: 410 4545 Fragments arranged by size: 4545 947 741 547 475 410 216 118 95 73 BamHI G'GATC_C Cuts at: 5497 5864 5497 Size: 367 7800 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3312 4275 4493 4583 4994 Size: 325 217 627 1525 963 218 90 411 Cuts at: 4994 5488 6943 618 Size: 494 1455 1842 Fragments arranged by size: 1842 1525 1455 963 627 494 411 325 218 217 90 BanII G_rGCy'C Cuts at: 1391 2337 3051 3705 3989 3996 4519 5486 1391 Size: 946 714 654 284 7 523 967 4072 Fragments arranged by size: 4072 967 946 714 654 523 284 7 BbeI G_GCGC'C Cuts at: 3316 3316 Size: 8167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2266 2840 3465 3777 5066 928 Size: 971 367 574 625 312 1289 4029 Fragments arranged by size: 4029 1289 971 625 574 367 312 BbvCI CC'TCA_GC Cuts at: 4877 4877 Size: 8167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3745 3779 6358 6392 1903 Size: 34 50 34 1724 34 2579 34 3678 Fragments arranged by size: 3678 2579 1724 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6059 7269 6059 Size: 1210 6957 BclI T'GATC_A Cuts at: 1548 4311 5196 1548 Size: 2763 885 4519 Fragments arranged by size: 4519 2763 885 BfrBI ATG'CAT Cuts at: 5112 5112 Size: 8167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2379 2999 6778 Size: 107 122 71 631 605 707 620 3779 Cuts at: 6778 136 Size: 1525 Fragments arranged by size: 3779 1525 707 631 620 605 122 107 71 BglII A'GATC_T Cuts at: 3802 8162 3802 Size: 4360 3807 BlpI GC'TnA_GC Cuts at: 2102 5584 2102 Size: 3482 4685 Bme1580I G_kGCm'C Cuts at: 1790 3585 3978 3989 3996 4083 4410 4496 4606 Size: 1795 393 11 7 87 327 86 110 Cuts at: 4606 6228 7384 1790 Size: 1622 1156 2573 Fragments arranged by size: 2573 1795 1622 1156 393 327 110 87 86 11 7 BmgBI CAC'GTC Cuts at: 1318 4411 1318 Size: 3093 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 5898 6856 Size: 416 53 83 399 24 917 3564 958 Cuts at: 6856 442 Size: 1753 Fragments arranged by size: 3564 1753 958 917 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2759 2831 2963 3047 3065 3140 3254 Size: 1262 258 72 132 84 18 75 114 Cuts at: 3254 3317 3923 4342 5186 5354 6827 1239 Size: 63 606 419 844 168 1473 2579 Fragments arranged by size: 2579 1473 1262 844 606 419 258 168 132 114 84 75 72 63 18 Bpu10I CC'TnA_GC Cuts at: 4877 4877 Size: 8167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3035 6292 7157 7455 3035 Size: 3257 865 298 3747 Fragments arranged by size: 3747 3257 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2667 5060 6830 882 Size: 617 1168 2393 1770 2219 Fragments arranged by size: 2393 2219 1770 1168 617 BsaAI yAC'GTr Cuts at: 493 1204 3979 5958 493 Size: 711 2775 1979 2702 Fragments arranged by size: 2775 2702 1979 711 BsaBI GATnn'nnATC Cuts at: 3257 8161 3257 Size: 4904 3263 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 3313 3603 3795 Size: 53 83 186 936 531 1249 290 192 Cuts at: 3795 4371 4812 5974 6356 6991 275 Size: 576 441 1162 382 635 1451 Fragments arranged by size: 1451 1249 1162 936 635 576 531 441 382 290 192 186 83 53 BsaWI w'CCGG_w Cuts at: 2706 4278 4608 6600 6998 7272 7419 7830 2706 Size: 1572 330 1992 398 274 147 411 3043 Fragments arranged by size: 3043 1992 1572 411 398 330 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2840 2870 3038 3068 3845 Size: 30 165 30 1437 30 168 30 777 Cuts at: 3845 3875 5282 5312 1178 Size: 30 1407 30 4033 Fragments arranged by size: 4033 1437 1407 777 168 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 2657 2680 2738 3002 3020 3044 3137 Size: 842 629 23 58 264 18 24 93 Cuts at: 3137 3251 3461 3835 3847 3850 3853 3856 4517 Size: 114 210 374 12 3 3 3 661 Cuts at: 4517 4586 5306 5309 5405 5441 1186 Size: 69 720 3 96 36 3912 Fragments arranged by size: 3912 842 720 661 629 374 264 210 114 96 93 69 58 36 24 23 18 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 3492 4038 4051 4502 4518 4864 5171 7370 7866 Size: 546 13 451 16 346 307 2199 496 Cuts at: 7866 3492 Size: 3793 Fragments arranged by size: 3793 2199 546 496 451 346 307 16 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4022 4235 4252 4360 4991 5167 1163 Size: 573 2286 213 17 108 631 176 4163 Fragments arranged by size: 4163 2286 631 573 213 176 108 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 5617 6378 7406 1540 Size: 275 186 928 2688 761 1028 2301 Fragments arranged by size: 2688 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3051 3059 4083 4410 4938 5226 6228 6313 7384 Size: 8 1024 327 528 288 1002 85 1071 Cuts at: 7384 3051 Size: 3834 Fragments arranged by size: 3834 1071 1024 1002 528 327 288 85 8 BsmI GAATG_Cn' Cuts at: 5114 5678 5771 5114 Size: 564 93 7510 Fragments arranged by size: 7510 564 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 4673 5279 2036 Size: 2637 606 4924 Fragments arranged by size: 4924 2637 606 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 3051 3059 3585 3705 3978 3989 Size: 399 547 714 8 526 120 273 11 Cuts at: 3989 3996 4083 4410 4496 4519 4606 4938 5226 Size: 7 87 327 86 23 87 332 288 Cuts at: 5226 5486 6228 6313 7384 1391 Size: 260 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 547 526 399 332 327 288 273 260 120 87 87 86 85 23 11 8 7 BspEI T'CCGG_A Cuts at: 4608 7830 4608 Size: 3222 4945 BspHI T'CATG_A Cuts at: 2661 6056 7786 2661 Size: 3395 1730 3042 Fragments arranged by size: 3395 3042 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2451 2788 2988 3369 3898 4707 5522 Size: 1304 303 337 200 381 529 809 815 Cuts at: 5522 844 Size: 3489 Fragments arranged by size: 3489 1304 815 809 529 381 337 303 200 BsrBI CCG'CTC Cuts at: 1394 1823 2539 2563 2815 3133 3139 4756 6054 Size: 429 716 24 252 318 6 1617 1298 Cuts at: 6054 1394 Size: 3507 Fragments arranged by size: 3507 1617 1298 716 429 318 252 24 6 BsrDI GCAATG_nn' Cuts at: 65 1248 6662 6836 65 Size: 1183 5414 174 1396 Fragments arranged by size: 5414 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2226 2706 4115 4926 5313 6811 Size: 110 281 135 480 1409 811 387 1498 Cuts at: 6811 6998 1700 Size: 187 2869 Fragments arranged by size: 2869 1498 1409 811 480 387 281 187 135 110 BsrGI T'GTAC_A Cuts at: 96 2310 3084 96 Size: 2214 774 5179 Fragments arranged by size: 5179 2214 774 BssHII G'CGCG_C Cuts at: 1925 4452 1925 Size: 2527 5640 BssSI C'ACGA_G Cuts at: 2375 3584 4439 6227 7239 2375 Size: 1209 855 1788 1012 3303 Fragments arranged by size: 3303 1788 1209 1012 855 BstAPI GCAn_nnn'nTGC Cuts at: 794 4978 5161 794 Size: 4184 183 3800 Fragments arranged by size: 4184 3800 183 BstBI TT'CG_AA Cuts at: 5106 6971 5106 Size: 1865 6302 BstEII G'GTnAC_C Cuts at: 1184 3961 1184 Size: 2777 5390 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2362 4366 4954 4966 5449 2359 Size: 3 2004 588 12 483 5077 Fragments arranged by size: 5077 2004 588 483 12 3 BstYI r'GATC_y Cuts at: 1972 2970 3222 3623 3802 5497 5864 6249 6266 Size: 998 252 401 179 1695 367 385 17 Cuts at: 6266 7718 7804 7816 8162 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1695 1452 998 401 385 367 346 252 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4998 5358 513 Size: 552 3933 360 3322 Fragments arranged by size: 3933 3322 552 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3737 5098 487 Size: 683 56 2511 1361 3556 Fragments arranged by size: 3556 2511 1361 683 56 BtsI GCAGTG_nn' Cuts at: 3194 5155 5683 6477 6497 3194 Size: 1961 528 794 20 4864 Fragments arranged by size: 4864 1961 794 528 20 ClaI AT'CG_AT Cuts at: 1554 2691 1554 Size: 1137 7030 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8132 DraI TTT'AAA Cuts at: 5471 5471 Size: 8167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2585 3722 4655 7174 809 Size: 507 1269 1137 933 2519 1802 Fragments arranged by size: 2519 1802 1269 1137 933 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 4104 4693 Size: 54 1425 50 275 18 633 1641 589 Cuts at: 4693 4956 5139 5614 6503 8132 8 Size: 263 183 475 889 1629 43 Fragments arranged by size: 1641 1629 1425 889 633 589 475 275 263 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5614 1537 Size: 275 3802 4090 Fragments arranged by size: 4090 3802 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2553 4836 4943 5231 6097 1353 Size: 699 501 2283 107 288 866 3423 Fragments arranged by size: 3423 2283 866 699 501 288 107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3477 6754 7128 7274 387 Size: 1064 138 1888 3277 374 146 1280 Fragments arranged by size: 3277 1888 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3049 3049 Size: 8167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2501 2759 2783 2831 2852 2963 Size: 393 437 432 258 24 48 21 111 Cuts at: 2963 3047 3065 3140 3254 3317 3477 3530 3572 Size: 84 18 75 114 63 160 53 42 Cuts at: 3572 3923 4342 4409 4688 5186 5339 5354 6230 Size: 351 419 67 279 498 153 15 876 Cuts at: 6230 6827 7614 7882 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 876 787 597 498 437 432 419 393 351 279 268 258 160 153 114 111 84 75 67 63 53 48 42 24 21 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 2984 3068 3320 3905 4124 5165 2984 Size: 84 252 585 219 1041 5986 Fragments arranged by size: 5986 1041 585 252 219 84 EcoO109I rG'GnC_Cy Cuts at: 2380 2440 2938 3040 3883 3985 3992 3993 5338 Size: 60 498 102 843 102 7 1 1345 Cuts at: 5338 2380 Size: 5209 Fragments arranged by size: 5209 1345 843 498 102 102 60 7 1 EcoRI G'AATT_C Cuts at: 5476 5854 5476 Size: 378 7789 EcoRV GAT'ATC Cuts at: 5026 5026 Size: 8167 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4652 4652 Size: 8167 FspI TGC'GCA Cuts at: 6673 6673 Size: 8167 HaeII r_GCGC'y Cuts at: 1678 3316 4549 4637 4924 7314 1678 Size: 1638 1233 88 287 2390 2531 Fragments arranged by size: 2531 2390 1638 1233 287 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2840 2872 3400 3432 5181 Size: 32 135 32 1268 32 528 32 1749 Cuts at: 5181 5213 6808 6840 6882 6914 1373 Size: 32 1595 32 42 32 2626 Fragments arranged by size: 2626 1749 1595 1268 528 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2965 3292 3913 4264 4462 5511 Size: 988 281 1027 327 621 351 198 1049 Cuts at: 5511 5757 669 Size: 246 3079 Fragments arranged by size: 3079 1049 1027 988 621 351 327 281 246 198 HindIII A'AGCT_T Cuts at: 748 4681 748 Size: 3933 4234 HpaI GTT'AAC Cuts at: 5757 5757 Size: 8167 KasI G'GCGC_C Cuts at: 3312 3312 Size: 8167 KpnI G_GTAC'C Cuts at: 4279 5492 4279 Size: 1213 6954 MfeI C'AATT_G Cuts at: 5766 5766 Size: 8167 MluI A'CGCG_T Cuts at: 5100 8043 5100 Size: 2943 5224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2214 2704 4167 5229 7281 7465 1305 Size: 909 490 1463 1062 2052 184 2007 Fragments arranged by size: 2052 2007 1463 1062 909 490 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 4695 4958 10 Size: 54 1425 343 633 2230 263 3219 Fragments arranged by size: 3219 2230 1425 633 343 263 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2360 3107 3653 3968 4413 5234 Size: 648 1191 3 747 546 315 445 821 Cuts at: 5234 5447 6125 6484 6643 518 Size: 213 678 359 159 2042 Fragments arranged by size: 2042 1191 821 747 678 648 546 445 359 315 213 159 3 MspA1I CmG'CkG Cuts at: 1606 2479 3118 3523 3783 4138 4236 4366 5464 Size: 873 639 405 260 355 98 130 1098 Cuts at: 5464 5583 6260 7408 7653 1606 Size: 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 1098 873 677 639 405 355 260 245 130 119 98 NaeI GCC'GGC Cuts at: 2228 4117 4928 5315 2228 Size: 1889 811 387 5080 Fragments arranged by size: 5080 1889 811 387 NarI GG'CG_CC Cuts at: 3313 3313 Size: 8167 NcoI C'CATG_G Cuts at: 513 1065 4998 513 Size: 552 3933 3682 Fragments arranged by size: 3933 3682 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8167 NgoMIV G'CCGG_C Cuts at: 2226 4115 4926 5313 2226 Size: 1889 811 387 5080 Fragments arranged by size: 5080 1889 811 387 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8167 NotI GC'GGCC_GC Cuts at: 5614 5614 Size: 8167 NruI TCG'CGA Cuts at: 4675 4675 Size: 8167 NsiI A_TGCA'T Cuts at: 5114 5114 Size: 8167 NspI r_CATG'y Cuts at: 1132 1229 2101 4197 5377 5525 7070 1132 Size: 97 872 2096 1180 148 1545 2229 Fragments arranged by size: 2229 2096 1545 1180 872 148 97 PasI CC'CwG_GG Cuts at: 1348 3064 3989 1348 Size: 1716 925 5526 Fragments arranged by size: 5526 1716 925 PciI A'CATG_T Cuts at: 5373 7066 5373 Size: 1693 6474 PflMI CCAn_nnn'nTGG Cuts at: 1108 4483 1108 Size: 3375 4792 PfoI T'CCnGG_A Cuts at: 2532 2907 2532 Size: 375 7792 PmeI GTTT'AAAC Cuts at: 5471 5471 Size: 8167 PmlI CAC'GTG Cuts at: 3979 3979 Size: 8167 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5282 5314 6236 6268 7749 7781 5282 Size: 32 922 32 1481 32 5668 Fragments arranged by size: 5668 1481 922 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 3040 2440 Size: 600 7567 PshAI GACnn'nnGTC Cuts at: 1613 3345 1613 Size: 1732 6435 PsiI TTA'TAA Cuts at: 5737 5737 Size: 8167 PspOMI G'GGCC_C Cuts at: 3985 3992 3985 Size: 7 8160 PstI C_TGCA'G Cuts at: 830 2421 2856 2985 3117 3528 4545 4623 5519 Size: 1591 435 129 132 411 1017 78 896 Cuts at: 5519 830 Size: 3478 Fragments arranged by size: 3478 1591 1017 896 435 411 132 129 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8167 PvuII CAG'CTG Cuts at: 2479 3118 3523 4138 4366 5464 2479 Size: 639 405 615 228 1098 5182 Fragments arranged by size: 5182 1098 639 615 405 228 SacI G_AGCT'C Cuts at: 3051 3051 Size: 8167 SalI G'TCGA_C Cuts at: 1936 4460 5509 1936 Size: 2524 1049 4594 Fragments arranged by size: 4594 2524 1049 SapI GCTCTTCn'nnn_ Cuts at: 2553 4836 4943 5231 2553 Size: 2283 107 288 5489 Fragments arranged by size: 5489 2283 288 107 SbfI CC_TGCA'GG Cuts at: 2985 5519 2985 Size: 2534 5633 ScaI AGT'ACT Cuts at: 3182 3470 6415 8127 3182 Size: 288 2945 1712 3222 Fragments arranged by size: 3222 2945 1712 288 SfcI C'TryA_G Cuts at: 826 1045 2417 2852 2981 3113 3524 4507 4541 Size: 219 1372 435 129 132 411 983 34 Cuts at: 4541 4619 4891 5515 6650 7331 7522 826 Size: 78 272 624 1135 681 191 1471 Fragments arranged by size: 1471 1372 1135 983 681 624 435 411 272 219 191 132 129 78 34 SfiI GGCCn_nnn'nGGCC Cuts at: 2379 2379 Size: 8167 SfoI GGC'GCC Cuts at: 3314 3314 Size: 8167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8167 SmaI CCC'GGG Cuts at: 1872 3708 4655 5494 1872 Size: 1836 947 839 4545 Fragments arranged by size: 4545 1836 947 839 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 3050 6271 7172 7434 820 Size: 197 926 741 366 3221 901 262 1553 Fragments arranged by size: 3221 1553 926 901 741 366 262 197 SnaBI TAC'GTA Cuts at: 493 5958 493 Size: 5465 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8167 SphI G_CATG'C Cuts at: 1132 5525 1132 Size: 4393 3774 SspI AAT'ATT Cuts at: 5 52 6091 5 Size: 47 6039 2081 Fragments arranged by size: 6039 2081 47 StuI AGG'CCT Cuts at: 1517 3149 3281 1517 Size: 1632 132 6403 Fragments arranged by size: 6403 1632 132 StyI C'CwwG_G Cuts at: 513 1065 2250 2436 3321 3576 4998 5606 513 Size: 552 1185 186 885 255 1422 608 3074 Fragments arranged by size: 3074 1422 1185 885 608 552 255 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3949 4075 4601 4960 5437 6194 6211 Size: 1255 2369 126 526 359 477 757 17 Cuts at: 6211 6364 6549 6994 325 Size: 153 185 445 1498 Fragments arranged by size: 2369 1498 1255 757 526 477 445 359 185 153 126 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 3084 3180 Size: 274 80 33 51 1443 333 774 96 Cuts at: 3180 3210 3468 6413 8125 96 Size: 30 258 2945 1712 138 Fragments arranged by size: 2945 1712 1443 774 333 274 258 138 96 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2054 2086 3038 3070 3845 3877 8127 8159 2054 Size: 32 952 32 775 32 4250 32 2062 Fragments arranged by size: 4250 2062 952 775 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8167 XbaI T'CTAG_A Cuts at: 5503 5503 Size: 8167 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2359 Size: 8167 XhoI C'TCGA_G Cuts at: 1943 2684 1943 Size: 741 7426 XmaI C'CCGG_G Cuts at: 1870 3706 4653 5492 1870 Size: 1836 947 839 4545 Fragments arranged by size: 4545 1836 947 839 XmnI GAAnn'nnTTC Cuts at: 6296 6296 Size: 8167 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5975 276 Size: 53 83 186 936 4441 2468 Fragments arranged by size: 4441 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I DraIII FseI FspAI PacI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI