Restriction map for
pFN21AF0120
(Linear) (Six-base) MAPSORT of: FLJ00120.seq Check: 7625 from: 1 to: 3462
FLJ00120.orf
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AccI GT'mk_AC
Cuts at: 0 2456 2573 3462
Size: 2456 117 889
Fragments arranged by size:
2456 889 117
Acc65I G'GTAC_C
Cuts at: 0 2270 3462
Size: 2270 1192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 778 847 1472 1525 1567 2404 2683
Size: 64 714 69 625 53 42 837 279
Cuts at: 2683 3334 3462
Size: 651 128
Fragments arranged by size:
837 714 651 625 279 128 69 64
53 42
AfeI AGC'GCT
Cuts at: 0 2630 3462
Size: 2630 832
AflIII A'CryG_T
Cuts at: 0 3095 3368 3462
Size: 3095 273 94
Fragments arranged by size:
3095 273 94
AgeI A'CCGG_T
Cuts at: 0 701 3462
Size: 701 2761
AhdI GACnn_n'nnGTC
Cuts at: 0 1409 3462
Size: 1409 2053
AleI CACnn'nnGTG
Cuts at: 0 352 1963 2408 3462
Size: 352 1611 445 1054
Fragments arranged by size:
1611 1054 445 352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1534 3462
Size: 1534 1928
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3277 3309 3462
Size: 3277 32 153
Fragments arranged by size:
3277 153 32
AlwNI CAG_nnn'CTG
Cuts at: 0 798 846 954 1344 2136 2544 2973 3462
Size: 798 48 108 390 792 408 429 489
Fragments arranged by size:
798 792 489 429 408 390 108 48
ApaI G_GGCC'C
Cuts at: 0 1984 1991 3462
Size: 1984 7 1471
Fragments arranged by size:
1984 1471 7
ApaLI G'TGCA_C
Cuts at: 0 2074 2401 3462
Size: 2074 327 1061
Fragments arranged by size:
2074 1061 327
ApoI r'AATT_y
Cuts at: 0 286 3462
Size: 286 3176
AvaI C'yCGr_G
Cuts at: 0 679 1154 1701 2648 2766 2982 3077 3462
Size: 679 475 547 947 118 216 95 385
Fragments arranged by size:
947 679 547 475 385 216 118 95
BanI G'GyrC_C
Cuts at: 0 1307 2270 2488 2578 2989 3462
Size: 1307 963 218 90 411 473
Fragments arranged by size:
1307 963 473 411 218 90
BanII G_rGCy'C
Cuts at: 0 332 1046 1700 1984 1991 2514 3462
Size: 332 714 654 284 7 523 948
Fragments arranged by size:
948 714 654 523 332 284 7
BbeI G_GCGC'C
Cuts at: 0 1311 3462
Size: 1311 2151
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 835 1460 1772 3061 3462
Size: 261 574 625 312 1289 401
Fragments arranged by size:
1289 625 574 401 312 261
BbvCI CC'TCA_GC
Cuts at: 0 2872 3462
Size: 2872 590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1740 1774 3462
Size: 1740 34 1688
Fragments arranged by size:
1740 1688 34
BclI T'GATC_A
Cuts at: 0 2306 3191 3462
Size: 2306 885 271
Fragments arranged by size:
2306 885 271
BfrBI ATG'CAT
Cuts at: 0 3107 3462
Size: 3107 355
BglI GCCn_nnn'nGGC
Cuts at: 0 374 994 3462
Size: 374 620 2468
Fragments arranged by size:
2468 620 374
BglII A'GATC_T
Cuts at: 0 1797 3462
Size: 1797 1665
BlpI GC'TnA_GC
Cuts at: 0 97 3462
Size: 97 3365
Bme1580I G_kGCm'C
Cuts at: 0 1580 1973 1984 1991 2078 2405 2491 2601
Size: 1580 393 11 7 87 327 86 110
Cuts at: 2601 3462
Size: 861
Fragments arranged by size:
1580 861 393 327 110 87 86 11
7
BmgBI CAC'GTC
Cuts at: 0 2406 3462
Size: 2406 1056
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 3462
Size: 329 3133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 754 826 958 1042 1060 1135 1249
Size: 496 258 72 132 84 18 75 114
Cuts at: 1249 1312 1918 2337 3181 3349 3462
Size: 63 606 419 844 168 113
Fragments arranged by size:
844 606 496 419 258 168 132 114
113 84 75 72 63 18
Bpu10I CC'TnA_GC
Cuts at: 0 2872 3462
Size: 2872 590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 3462
Size: 1030 2432
BsaI GGTCTCn'nnnn_
Cuts at: 0 662 3055 3462
Size: 662 2393 407
Fragments arranged by size:
2393 662 407
BsaAI yAC'GTr
Cuts at: 0 1974 3462
Size: 1974 1488
BsaBI GATnn'nnATC
Cuts at: 0 1252 3462
Size: 1252 2210
BsaHI Gr'CG_yC
Cuts at: 0 59 1308 1598 1790 2366 2807 3462
Size: 59 1249 290 192 576 441 655
Fragments arranged by size:
1249 655 576 441 290 192 59
BsaWI w'CCGG_w
Cuts at: 0 701 2273 2603 3462
Size: 701 1572 330 859
Fragments arranged by size:
1572 859 701 330
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 835 865 1033 1063 1840 1870 3277 3307
Size: 835 30 168 30 777 30 1407 30
Cuts at: 3307 3462
Size: 155
Fragments arranged by size:
1407 835 777 168 155 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 652 675 733 997 1015 1039 1132
Size: 23 629 23 58 264 18 24 93
Cuts at: 1132 1246 1456 1830 1842 1845 1848 1851 2512
Size: 114 210 374 12 3 3 3 661
Cuts at: 2512 2581 3301 3304 3400 3436 3462
Size: 69 720 3 96 36 26
Fragments arranged by size:
720 661 629 374 264 210 114 96
93 69 58 36 26 24 23 23
18 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1487 2033 2046 2497 2513 2859 3166 3462
Size: 1487 546 13 451 16 346 307 296
Fragments arranged by size:
1487 546 451 346 307 296 16 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2017 2230 2247 2355 2986 3162 3462
Size: 2017 213 17 108 631 176 300
Fragments arranged by size:
2017 631 300 213 176 108 17
BsiEI CG_ry'CG
Cuts at: 0 924 3462
Size: 924 2538
BsiHKAI G_wGCw'C
Cuts at: 0 1046 1054 2078 2405 2933 3221 3462
Size: 1046 8 1024 327 528 288 241
Fragments arranged by size:
1046 1024 528 327 288 241 8
BsmI GAATG_Cn'
Cuts at: 0 3109 3462
Size: 3109 353
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 2668 3274 3462
Size: 31 2637 606 188
Fragments arranged by size:
2637 606 188 31
Bsp1286I G_dGCh'C
Cuts at: 0 332 1046 1054 1580 1700 1973 1984 1991
Size: 332 714 8 526 120 273 11 7
Cuts at: 1991 2078 2405 2491 2514 2601 2933 3221 3462
Size: 87 327 86 23 87 332 288 241
Fragments arranged by size:
714 526 332 332 327 288 273 241
120 87 87 86 23 11 8 7
BspEI T'CCGG_A
Cuts at: 0 2603 3462
Size: 2603 859
BspHI T'CATG_A
Cuts at: 0 656 3462
Size: 656 2806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 446 783 983 1364 1893 2702 3462
Size: 143 303 337 200 381 529 809 760
Fragments arranged by size:
809 760 529 381 337 303 200 143
BsrBI CCG'CTC
Cuts at: 0 534 558 810 1128 1134 2751 3462
Size: 534 24 252 318 6 1617 711
Fragments arranged by size:
1617 711 534 318 252 24 6
BsrFI r'CCGG_y
Cuts at: 0 86 221 701 2110 2921 3308 3462
Size: 86 135 480 1409 811 387 154
Fragments arranged by size:
1409 811 480 387 154 135 86
BsrGI T'GTAC_A
Cuts at: 0 305 1079 3462
Size: 305 774 2383
Fragments arranged by size:
2383 774 305
BssHII G'CGCG_C
Cuts at: 0 2447 3462
Size: 2447 1015
BssSI C'ACGA_G
Cuts at: 0 370 1579 2434 3462
Size: 370 1209 855 1028
Fragments arranged by size:
1209 1028 855 370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2973 3156 3462
Size: 2973 183 306
Fragments arranged by size:
2973 306 183
BstBI TT'CG_AA
Cuts at: 0 3101 3462
Size: 3101 361
BstEII G'GTnAC_C
Cuts at: 0 1956 3462
Size: 1956 1506
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 357 2361 2949 2961 3444 3462
Size: 354 3 2004 588 12 483 18
Fragments arranged by size:
2004 588 483 354 18 12 3
BstYI r'GATC_y
Cuts at: 0 965 1217 1618 1797 3462
Size: 965 252 401 179 1665
Fragments arranged by size:
1665 965 401 252 179
BtgI C'CryG_G
Cuts at: 0 2993 3353 3462
Size: 2993 360 109
Fragments arranged by size:
2993 360 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1732 3093 3462
Size: 1732 1361 369
Fragments arranged by size:
1732 1361 369
BtsI GCAGTG_nn'
Cuts at: 0 1189 3150 3462
Size: 1189 1961 312
Fragments arranged by size:
1961 1189 312
ClaI AT'CG_AT
Cuts at: 0 686 3462
Size: 686 2776
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 1717 2650 3462
Size: 580 1137 933 812
Fragments arranged by size:
1137 933 812 580
EaeI y'GGCC_r
Cuts at: 0 458 2099 2688 2951 3134 3462
Size: 458 1641 589 263 183 328
Fragments arranged by size:
1641 589 458 328 263 183
EarI CTCTTCn'nnn_
Cuts at: 0 47 548 2831 2938 3226 3462
Size: 47 501 2283 107 288 236
Fragments arranged by size:
2283 501 288 236 107 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1472 3462
Size: 1472 1990
EcoICRI GAG'CTC
Cuts at: 0 1044 3462
Size: 1044 2418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 496 754 778 826 847 958 1042
Size: 64 432 258 24 48 21 111 84
Cuts at: 1042 1060 1135 1249 1312 1472 1525 1567 1918
Size: 18 75 114 63 160 53 42 351
Cuts at: 1918 2337 2404 2683 3181 3334 3349 3462
Size: 419 67 279 498 153 15 113
Fragments arranged by size:
498 432 419 351 279 258 160 153
114 113 111 84 75 67 64 63
53 48 42 24 21 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 979 1063 1315 1900 2119 3160 3462
Size: 979 84 252 585 219 1041 302
Fragments arranged by size:
1041 979 585 302 252 219 84
EcoO109I rG'GnC_Cy
Cuts at: 0 375 435 933 1035 1878 1980 1987 1988
Size: 375 60 498 102 843 102 7 1
Cuts at: 1988 3333 3462
Size: 1345 129
Fragments arranged by size:
1345 843 498 375 129 102 102 60
7 1
EcoRV GAT'ATC
Cuts at: 0 3021 3462
Size: 3021 441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2647 3462
Size: 2647 815
HaeII r_GCGC'y
Cuts at: 0 1311 2544 2632 2919 3462
Size: 1311 1233 88 287 543
Fragments arranged by size:
1311 1233 543 287 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 1395 1427 3176 3208 3462
Size: 835 32 528 32 1749 32 254
Fragments arranged by size:
1749 835 528 254 32 32 32
HincII GTy'rAC
Cuts at: 0 960 1287 1908 2259 2457 3462
Size: 960 327 621 351 198 1005
Fragments arranged by size:
1005 960 621 351 327 198
HindIII A'AGCT_T
Cuts at: 0 2676 3462
Size: 2676 786
KasI G'GCGC_C
Cuts at: 0 1307 3462
Size: 1307 2155
KpnI G_GTAC'C
Cuts at: 0 2274 3462
Size: 2274 1188
MluI A'CGCG_T
Cuts at: 0 3095 3462
Size: 3095 367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 699 2162 3224 3462
Size: 209 490 1463 1062 238
Fragments arranged by size:
1463 1062 490 238 209
MscI TGG'CCA
Cuts at: 0 460 2690 2953 3462
Size: 460 2230 263 509
Fragments arranged by size:
2230 509 460 263
MslI CAynn'nnrTG
Cuts at: 0 352 355 1102 1648 1963 2408 3229 3442
Size: 352 3 747 546 315 445 821 213
Cuts at: 3442 3462
Size: 20
Fragments arranged by size:
821 747 546 445 352 315 213 20
3
MspA1I CmG'CkG
Cuts at: 0 474 1113 1518 1778 2133 2231 2361 3459
Size: 474 639 405 260 355 98 130 1098
Cuts at: 3459 3462
Size: 3
Fragments arranged by size:
1098 639 474 405 355 260 130 98
3
NaeI GCC'GGC
Cuts at: 0 223 2112 2923 3310 3462
Size: 223 1889 811 387 152
Fragments arranged by size:
1889 811 387 223 152
NarI GG'CG_CC
Cuts at: 0 1308 3462
Size: 1308 2154
NcoI C'CATG_G
Cuts at: 0 2993 3462
Size: 2993 469
NgoMIV G'CCGG_C
Cuts at: 0 221 2110 2921 3308 3462
Size: 221 1889 811 387 154
Fragments arranged by size:
1889 811 387 221 154
NruI TCG'CGA
Cuts at: 0 2670 3462
Size: 2670 792
NsiI A_TGCA'T
Cuts at: 0 3109 3462
Size: 3109 353
NspI r_CATG'y
Cuts at: 0 96 2192 3372 3462
Size: 96 2096 1180 90
Fragments arranged by size:
2096 1180 96 90
PasI CC'CwG_GG
Cuts at: 0 1059 1984 3462
Size: 1059 925 1478
Fragments arranged by size:
1478 1059 925
PciI A'CATG_T
Cuts at: 0 3368 3462
Size: 3368 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 2478 3462
Size: 2478 984
PfoI T'CCnGG_A
Cuts at: 0 527 902 3462
Size: 527 375 2560
Fragments arranged by size:
2560 527 375
PmlI CAC'GTG
Cuts at: 0 1974 3462
Size: 1974 1488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3277 3309 3462
Size: 3277 32 153
Fragments arranged by size:
3277 153 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 1035 3462
Size: 435 600 2427
Fragments arranged by size:
2427 600 435
PshAI GACnn'nnGTC
Cuts at: 0 1340 3462
Size: 1340 2122
PspOMI G'GGCC_C
Cuts at: 0 1980 1987 3462
Size: 1980 7 1475
Fragments arranged by size:
1980 1475 7
PstI C_TGCA'G
Cuts at: 0 416 851 980 1112 1523 2540 2618 3462
Size: 416 435 129 132 411 1017 78 844
Fragments arranged by size:
1017 844 435 416 411 132 129 78
PvuII CAG'CTG
Cuts at: 0 474 1113 1518 2133 2361 3459 3462
Size: 474 639 405 615 228 1098 3
Fragments arranged by size:
1098 639 615 474 405 228 3
SacI G_AGCT'C
Cuts at: 0 1046 3462
Size: 1046 2416
SalI G'TCGA_C
Cuts at: 0 2455 3462
Size: 2455 1007
SapI GCTCTTCn'nnn_
Cuts at: 0 548 2831 2938 3226 3462
Size: 548 2283 107 288 236
Fragments arranged by size:
2283 548 288 236 107
SbfI CC_TGCA'GG
Cuts at: 0 980 3462
Size: 980 2482
ScaI AGT'ACT
Cuts at: 0 1177 1465 3462
Size: 1177 288 1997
Fragments arranged by size:
1997 1177 288
SfcI C'TryA_G
Cuts at: 0 412 847 976 1108 1519 2502 2536 2614
Size: 412 435 129 132 411 983 34 78
Cuts at: 2614 2886 3462
Size: 272 576
Fragments arranged by size:
983 576 435 412 411 272 132 129
78 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 374 3462
Size: 374 3088
SfoI GGC'GCC
Cuts at: 0 1309 3462
Size: 1309 2153
SmaI CCC'GGG
Cuts at: 0 1703 2650 3462
Size: 1703 947 812
Fragments arranged by size:
1703 947 812
SmlI C'TyrA_G
Cuts at: 0 679 1045 3462
Size: 679 366 2417
Fragments arranged by size:
2417 679 366
StuI AGG'CCT
Cuts at: 0 1144 1276 3462
Size: 1144 132 2186
Fragments arranged by size:
2186 1144 132
StyI C'CwwG_G
Cuts at: 0 245 431 1316 1571 2993 3462
Size: 245 186 885 255 1422 469
Fragments arranged by size:
1422 885 469 255 245 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1944 2070 2596 2955 3432 3462
Size: 1944 126 526 359 477 30
Fragments arranged by size:
1944 526 477 359 126 30
TatI w'GTAC_w
Cuts at: 0 305 1079 1175 1205 1463 3462
Size: 305 774 96 30 258 1999
Fragments arranged by size:
1999 774 305 258 96 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 1033 1065 1840 1872 3462
Size: 49 32 952 32 775 32 1590
Fragments arranged by size:
1590 952 775 49 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 3462
Size: 354 3108
XhoI C'TCGA_G
Cuts at: 0 679 3462
Size: 679 2783
XmaI C'CCGG_G
Cuts at: 0 1701 2648 3462
Size: 1701 947 814
Fragments arranged by size:
1701 947 814
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AscI AseI AsiSI AvrII
BaeI BamHI BciVI BmtI BplI BsiWI BsrDI BstZ17I
Bsu36I CspCI DraI DraIII EagI EcoRI FseI FspI
FspAI HpaI MfeI NdeI NheI NotI PacI PmeI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI