Restriction map for
pFN21AA1121
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AflII Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbrPI BfrI
BlnI BsaBI BsaI BsiWI BspDI
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI DraI DraIII Eco47III
EcoO65I EcoRI EcoRV EcoT22I KpnI
MamI MluI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI PvuI
SbfI ScaI SfiI SmlI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 938 GACGTC GACGT^C
AccIII 3953 TCCGGA T^CCGGA
AclI 2330 AACGTT AA^CGTT
AgeI 473 ACCGGT A^CCGGT
AhdI 7 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1696 CCTCAGG CC^T[ACGT]AGG
AspEI 7 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 656 TGCGCA TGC^GCA
BcgI 2176 CGACATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 245 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 699 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 699 GCTGAGC GC^T[ACGT]AGC
BsaAI 3697 TACGTG [CT]AC^GT[GA]
BseAI 3953 TCCGGA T^CCGGA
BsmI 1569 GCATTC GAATGC(1/-1)
BspEI 3953 TCCGGA T^CCGGA
BspHI 527 TCATGA T^CATGA
BsrDI 2894 GCAATG GCAATG(2/0)
BstBI 856 TTCGAA TT^CGAA
Bsu36I 1696 CCTCAGG CC^T[ACGT]AGG
CelII 699 GCTGAGC GC^T[ACGT]AGC
CpoI 3821 CGGACCG CG^G[AT]CCG
DrdI 2769 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 7 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1696 CCTCAGG CC^T[ACGT]AGG
EcoNI 681 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 259 GGCCGGCC GGCCGG^CC
FspI 656 TGCGCA TGC^GCA
HindIII 2632 AAGCTT A^AGCTT
HpaI 3097 GTTAAC GTT^AAC
KspI 751 CCGCGG CCGC^GG
MroI 3953 TCCGGA T^CCGGA
NcoI 2280 CCATGG C^CATGG
NspV 856 TTCGAA TT^CGAA
PflMI 504 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 473 ACCGGT A^CCGGT
Psp1406I 2330 AACGTT AA^CGTT
RcaI 527 TCATGA T^CATGA
RsrII 3821 CGGACCG CG^G[AT]CCG
SacII 751 CCGCGG CCGC^GG
SalI 2411 GTCGAC G^TCGAC
SapI 564 GAAGAGC GCTCTTC(1/4)
SexAI 3811 ACCTGGT A^CC[AT]GGT
SfuI 856 TTCGAA TT^CGAA
SgrAI 156 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2661 ACTAGT A^CTAGT
Van91I 504 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 451 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2854 CAGCTGCTG
Asp700I 1380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2671 GAAGTTCTTC
AvaI 620 CCCGGG C^[CT]CG[GA]G
3673 CCCGGG
BbvCI 681 CCTCAGC CCTCAGC(-5/-2)
1936 CCTCAGC
BciVI 991 GTATCC GTATCC(6/5)
1075 GTATCC
BclI 1299 TGATCA T^GATCA
3774 TGATCA
BglII 1518 AGATCT A^GATCT
3195 AGATCT
BsmBI 3961 CGTCTC CGTCTC(1/5)
2767 GAGACG
BsoBI 620 CCCGGG C^[CT]CG[GA]G
3673 CCCGGG
Bsp1407I 1013 TGTACA T^GTACA
3755 TGTACA
BspLU11I 1010 ACATGT A^CATGT
2709 ACATGT
BsrGI 1013 TGTACA T^GTACA
3755 TGTACA
BssHII 89 GCGCGC G^CGCGC
91 GCGCGC
EagI 125 CGGCCG C^GGCCG
258 CGGCCG
EclXI 125 CGGCCG C^GGCCG
258 CGGCCG
Eco52I 125 CGGCCG C^GGCCG
258 CGGCCG
FbaI 1299 TGATCA T^GATCA
3774 TGATCA
MfeI 1711 CAATTG C^AATTG
2972 CAATTG
MunI 1711 CAATTG C^AATTG
2972 CAATTG
PshAI 1896 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3702 GACTTTCGTC
SacI 702 GAGCTC GAGCT^C
1976 GAGCTC
SmaI 620 CCCGGG CCC^GGG
3673 CCCGGG
SphI 1067 GCATGC GCATG^C
2760 GCATGC
SspBI 1013 TGTACA T^GTACA
3755 TGTACA
StuI 1224 AGGCCT AGG^CCT
2290 AGGCCT
XmaCI 620 CCCGGG C^CCGGG
3673 CCCGGG
XmaI 620 CCCGGG C^CCGGG
3673 CCCGGG
XmnI 1380 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2671 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII