(Circular) (Six-base) MAPSORT of: pFN21AA1121.seq Check: 7177 from: 1 to: 8764 pFN21AA1121 8764 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4929 4929 Size: 8764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2947 6574 278 Size: 53 83 186 936 1411 3627 2468 Fragments arranged by size: 3627 2468 1411 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2947 4417 4797 6107 1937 Size: 1010 1470 380 1310 4594 Fragments arranged by size: 4594 1470 1310 1010 380 Acc65I G'GTAC_C Cuts at: 6085 6085 Size: 8764 AclI AA'CG_TT Cuts at: 1563 4336 6891 7264 1563 Size: 2773 2555 373 3063 Fragments arranged by size: 3063 2773 2555 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 3053 4443 4667 5210 5277 5312 6827 Size: 956 465 1390 224 543 67 35 1515 Cuts at: 6827 8211 8479 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1515 1390 1384 956 543 465 268 224 67 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8567 AflIII A'CryG_T Cuts at: 1203 3015 3660 4714 7663 8640 1203 Size: 1812 645 1054 2949 977 1327 Fragments arranged by size: 2949 1812 1327 1054 977 645 AgeI A'CCGG_T Cuts at: 2478 7595 2478 Size: 5117 3647 AhdI GACnn_n'nnGTC Cuts at: 2017 7493 2017 Size: 5476 3288 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2087 1184 Size: 903 7861 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2856 2888 3155 3187 2856 Size: 32 267 32 8433 Fragments arranged by size: 8433 267 32 32 AlwNI CAG_nnn'CTG Cuts at: 2461 4864 6507 8079 2461 Size: 2403 1643 1572 3146 Fragments arranged by size: 3146 2403 1643 1572 ApaLI G'TGCA_C Cuts at: 6821 7977 6821 Size: 1156 7608 ApoI r'AATT_y Cuts at: 1493 2723 2981 4004 4907 6073 6299 6451 8560 Size: 1230 258 1023 903 1166 226 152 2109 Cuts at: 8560 1493 Size: 1697 Fragments arranged by size: 2109 1697 1230 1166 1023 903 258 226 152 AseI AT'TA_AT Cuts at: 160 1032 7318 160 Size: 872 6286 1606 Fragments arranged by size: 6286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 AvaI C'yCGr_G Cuts at: 1870 1943 2625 5678 6089 1870 Size: 73 682 3053 411 4545 Fragments arranged by size: 4545 3053 682 411 73 BamHI G'GATC_C Cuts at: 6094 6461 6094 Size: 367 8397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2165 3335 4226 5143 Size: 325 217 627 372 6 1170 891 917 Cuts at: 5143 5189 6085 7540 618 Size: 46 896 1455 1842 Fragments arranged by size: 1842 1455 1170 917 896 891 627 372 325 217 46 6 BanII G_rGCy'C Cuts at: 1391 2129 2711 3985 6083 1391 Size: 738 582 1274 2098 4072 Fragments arranged by size: 4072 2098 1274 738 582 BbeI G_GCGC'C Cuts at: 2163 2169 4230 2163 Size: 6 2061 6697 Fragments arranged by size: 6697 2061 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2626 6054 6063 928 Size: 971 727 3428 9 3629 Fragments arranged by size: 3629 3428 971 727 9 BbvCI CC'TCA_GC Cuts at: 2687 3942 2687 Size: 1255 7509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4170 4204 6955 6989 1903 Size: 34 50 34 2149 34 2751 34 3678 Fragments arranged by size: 3678 2751 2149 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3007 3091 6656 7866 3007 Size: 84 3565 1210 3905 Fragments arranged by size: 3905 3565 1210 84 BclI T'GATC_A Cuts at: 1548 3304 5779 1548 Size: 1756 2475 4533 Fragments arranged by size: 4533 2475 1756 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2256 7375 136 Size: 107 122 71 631 605 584 5119 1525 Fragments arranged by size: 5119 1525 631 605 584 122 107 71 BglII A'GATC_T Cuts at: 3523 5200 8759 3523 Size: 1677 3559 3528 Fragments arranged by size: 3559 3528 1677 BlpI GC'TnA_GC Cuts at: 2705 6181 2705 Size: 3476 5288 Bme1580I G_kGCm'C Cuts at: 1790 3338 3897 6825 7981 1790 Size: 1548 559 2928 1156 2573 Fragments arranged by size: 2928 2573 1548 1156 559 BmgBI CAC'GTC Cuts at: 1318 5689 1318 Size: 4371 4393 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4481 6495 7453 Size: 416 53 83 399 24 3064 2014 958 Cuts at: 7453 442 Size: 1753 Fragments arranged by size: 3064 2014 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2257 3089 4812 7424 1239 Size: 1018 832 1723 2612 2579 Fragments arranged by size: 2612 2579 1723 1018 832 Bpu10I CC'TnA_GC Cuts at: 2687 3942 2687 Size: 1255 7509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6889 7754 8052 6889 Size: 865 298 7601 Fragments arranged by size: 7601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7427 882 Size: 617 5928 2219 Fragments arranged by size: 5928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5704 6555 493 Size: 711 4500 851 2702 Fragments arranged by size: 4500 2702 851 711 BsaBI GATnn'nnATC Cuts at: 8758 8758 Size: 8764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 2166 2944 3408 Size: 53 83 186 936 627 6 778 464 Cuts at: 3408 4227 5220 5697 6571 6953 7588 275 Size: 819 993 477 874 382 635 1451 Fragments arranged by size: 1451 993 936 874 819 778 635 627 477 464 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 2478 5829 5958 7197 7595 7869 8016 8427 2478 Size: 3351 129 1239 398 274 147 411 2815 Fragments arranged by size: 3351 2815 1239 411 398 274 147 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2163 2193 2856 2886 3020 Size: 30 165 30 760 30 663 30 134 Cuts at: 3020 3050 3155 3185 3792 3822 3921 3951 5780 Size: 30 105 30 607 30 99 30 1829 Cuts at: 5780 5810 1178 Size: 30 4132 Fragments arranged by size: 4132 1829 760 663 607 165 134 105 99 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2069 2351 2354 2357 3041 3167 3300 Size: 880 3 282 3 3 684 126 133 Cuts at: 3300 4514 4709 5768 1186 Size: 1214 195 1059 4182 Fragments arranged by size: 4182 1214 1059 880 684 282 195 133 126 3 3 3 BseYI C'CCAG_C Cuts at: 2108 2146 2243 2279 3750 7967 8463 2108 Size: 38 97 36 1471 4217 496 2409 Fragments arranged by size: 4217 2409 1471 496 97 38 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2205 2564 3665 3694 5231 5797 1163 Size: 573 469 359 1101 29 1537 566 4130 Fragments arranged by size: 4130 1537 1101 573 566 469 359 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 2266 2578 6214 6975 8003 Size: 275 186 132 133 312 3636 761 1028 Cuts at: 8003 1540 Size: 2301 Fragments arranged by size: 3636 2301 1028 761 312 275 186 133 132 BsiHKAI G_wGCw'C Cuts at: 2076 2711 3404 3985 4701 6825 6910 7981 2076 Size: 635 693 581 716 2124 85 1071 2859 Fragments arranged by size: 2859 2124 1071 716 693 635 581 85 BsmI GAATG_Cn' Cuts at: 3574 6275 6368 3574 Size: 2701 93 5970 Fragments arranged by size: 5970 2701 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4766 5972 4766 Size: 1206 7558 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2129 2711 3338 3404 3897 3985 Size: 399 286 53 582 627 66 493 88 Cuts at: 3985 4701 6083 6825 6910 7981 1391 Size: 716 1382 742 85 1071 2174 Fragments arranged by size: 2174 1382 1071 742 716 627 582 493 399 286 88 85 66 53 BspEI T'CCGG_A Cuts at: 5958 8427 5958 Size: 2469 6295 BspHI T'CATG_A Cuts at: 2532 6653 8383 2532 Size: 4121 1730 2913 Fragments arranged by size: 4121 2913 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3057 3637 4929 5253 6119 844 Size: 2213 580 1292 324 866 3489 Fragments arranged by size: 3489 2213 1292 866 580 324 BsrBI CCG'CTC Cuts at: 1394 1823 2061 2323 5008 6651 1394 Size: 429 238 262 2685 1643 3507 Fragments arranged by size: 3507 2685 1643 429 262 238 BsrDI GCAATG_nn' Cuts at: 65 1248 4906 7259 7433 65 Size: 1183 3658 2353 174 1396 Fragments arranged by size: 3658 2353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2132 2162 2265 2433 2478 3076 4614 Size: 110 322 30 103 168 45 598 1538 Cuts at: 4614 7408 7595 1700 Size: 2794 187 2869 Fragments arranged by size: 2869 2794 1538 598 322 187 168 110 103 45 30 BsrGI T'GTAC_A Cuts at: 96 3018 5760 96 Size: 2922 2742 3100 Fragments arranged by size: 3100 2922 2742 BssHII G'CGCG_C Cuts at: 1925 2094 2096 1925 Size: 169 2 8593 Fragments arranged by size: 8593 169 2 BssSI C'ACGA_G Cuts at: 6824 7836 6824 Size: 1012 7752 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8764 BstBI TT'CG_AA Cuts at: 2862 7568 2862 Size: 4706 4058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8764 BstXI CCAn_nnnn'nTGG Cuts at: 2425 3007 4286 4836 5662 2425 Size: 582 1279 550 826 5527 Fragments arranged by size: 5527 1279 826 582 550 BstYI r'GATC_y Cuts at: 1972 3523 4960 5200 6094 6461 6846 6863 8315 Size: 1551 1437 240 894 367 385 17 1452 Cuts at: 8315 8401 8413 8759 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1551 1452 1437 894 385 367 346 240 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3702 3702 Size: 8764 BtgI C'CryG_G Cuts at: 513 1065 2740 2756 3870 4285 513 Size: 552 1675 16 1114 415 4992 Fragments arranged by size: 4992 1675 1114 552 415 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2374 6054 487 Size: 683 56 1148 3680 3197 Fragments arranged by size: 3680 3197 1148 683 56 BtsI GCAGTG_nn' Cuts at: 3365 6280 7074 7094 3365 Size: 2915 794 20 5035 Fragments arranged by size: 5035 2915 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3258 3293 529 Size: 35 2694 35 6000 Fragments arranged by size: 6000 2694 35 35 DraI TTT'AAA Cuts at: 6068 6068 Size: 8764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 7771 809 Size: 507 3464 2991 1802 Fragments arranged by size: 3464 2991 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2263 2718 Size: 54 1425 50 275 18 300 133 455 Cuts at: 2718 3057 3769 4123 5691 6211 7100 8729 8 Size: 339 712 354 1568 520 889 1629 43 Fragments arranged by size: 1629 1568 1425 889 712 520 455 354 339 300 275 133 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 2263 6211 1537 Size: 275 318 133 3948 4090 Fragments arranged by size: 4090 3948 318 275 133 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 2348 2564 2648 2742 3608 4018 4578 Size: 954 41 216 84 94 866 410 560 Cuts at: 4578 5256 6694 1353 Size: 678 1438 3423 Fragments arranged by size: 3423 1438 954 866 678 560 410 216 94 84 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2602 5705 7351 7725 7871 387 Size: 1064 138 1013 3103 1646 374 146 1280 Fragments arranged by size: 3103 1646 1280 1064 1013 374 146 138 EcoICRI GAG'CTC Cuts at: 2709 3983 2709 Size: 1274 7490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2257 2588 3053 3089 4443 4667 4812 Size: 393 625 331 465 36 1354 224 145 Cuts at: 4812 5210 5277 5312 6827 7424 8211 8479 1239 Size: 398 67 35 1515 597 787 268 1524 Fragments arranged by size: 1524 1515 1354 787 625 597 465 398 393 331 268 224 145 67 36 35 EcoNI CCTnn'n_nnAGG Cuts at: 2690 2690 Size: 8764 EcoO109I rG'GnC_Cy Cuts at: 2752 5242 5917 2752 Size: 2490 675 5599 Fragments arranged by size: 5599 2490 675 EcoRI G'AATT_C Cuts at: 6073 6451 6073 Size: 378 8386 FseI GG_CCGG'CC Cuts at: 2269 2269 Size: 8764 FspI TGC'GCA Cuts at: 2663 7270 2663 Size: 4607 4157 HaeII r_GCGC'y Cuts at: 1678 2142 2163 2169 4230 7911 1678 Size: 464 21 6 2061 3681 2531 Fragments arranged by size: 3681 2531 2061 464 21 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 3020 3052 4997 Size: 32 135 32 431 32 985 32 1945 Cuts at: 4997 5029 5168 5200 7405 7437 7479 7511 1373 Size: 32 139 32 2205 32 42 32 2626 Fragments arranged by size: 2626 2205 1945 985 431 139 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3046 4418 4549 5104 5823 6108 Size: 988 281 1108 1372 131 555 719 285 Cuts at: 6108 6354 669 Size: 246 3079 Fragments arranged by size: 3079 1372 1108 988 719 555 285 281 246 131 HindIII A'AGCT_T Cuts at: 748 4637 748 Size: 3889 4875 HpaI GTT'AAC Cuts at: 5104 6354 5104 Size: 1250 7514 KasI G'GCGC_C Cuts at: 2159 2165 4226 2159 Size: 6 2061 6697 Fragments arranged by size: 6697 2061 6 KpnI G_GTAC'C Cuts at: 6089 6089 Size: 8764 MfeI C'AATT_G Cuts at: 3716 4977 6363 3716 Size: 1261 1386 6117 Fragments arranged by size: 6117 1386 1261 MluI A'CGCG_T Cuts at: 8640 8640 Size: 8764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 3399 4041 7878 8062 1305 Size: 993 1101 642 3837 184 2007 Fragments arranged by size: 3837 2007 1101 993 642 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 3059 4125 10 Size: 54 1425 343 888 339 1066 4649 Fragments arranged by size: 4649 1425 1066 888 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3143 4284 5149 5468 5804 6026 Size: 648 1257 720 1141 865 319 336 222 Cuts at: 6026 6722 7081 7240 518 Size: 696 359 159 2042 Fragments arranged by size: 2042 1257 1141 865 720 696 648 359 336 319 222 159 MspA1I CmG'CkG Cuts at: 1606 2205 2217 2365 2461 2758 2893 3374 3946 Size: 599 12 148 96 297 135 481 572 Cuts at: 3946 4861 6180 6857 8005 8250 1606 Size: 915 1319 677 1148 245 2120 Fragments arranged by size: 2120 1319 1148 915 677 599 572 481 297 245 148 135 96 12 NaeI GCC'GGC Cuts at: 2134 2164 2267 2435 2134 Size: 30 103 168 8463 Fragments arranged by size: 8463 168 103 30 NarI GG'CG_CC Cuts at: 2160 2166 4227 2160 Size: 6 2061 6697 Fragments arranged by size: 6697 2061 6 NcoI C'CATG_G Cuts at: 513 1065 4285 513 Size: 552 3220 4992 Fragments arranged by size: 4992 3220 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8764 NgoMIV G'CCGG_C Cuts at: 2132 2162 2265 2433 2132 Size: 30 103 168 8463 Fragments arranged by size: 8463 168 103 30 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8764 NotI GC'GGCC_GC Cuts at: 6211 6211 Size: 8764 NspI r_CATG'y Cuts at: 1132 1229 3019 3076 4718 4769 6122 7667 1132 Size: 97 1790 57 1642 51 1353 1545 2229 Fragments arranged by size: 2229 1790 1642 1545 1353 97 57 51 PasI CC'CwG_GG Cuts at: 1348 3340 1348 Size: 1992 6772 PciI A'CATG_T Cuts at: 3015 4714 7663 3015 Size: 1699 2949 4116 Fragments arranged by size: 4116 2949 1699 PflMI CCAn_nnn'nTGG Cuts at: 1108 2515 1108 Size: 1407 7357 PfoI T'CCnGG_A Cuts at: 2711 5919 2711 Size: 3208 5556 PmeI GTTT'AAAC Cuts at: 6068 6068 Size: 8764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2856 2888 3155 3187 6833 6865 8346 8378 2856 Size: 32 267 32 3646 32 1481 32 3242 Fragments arranged by size: 3646 3242 1481 267 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2752 5242 5917 2752 Size: 2490 675 5599 Fragments arranged by size: 5599 2490 675 PshAI GACnn'nnGTC Cuts at: 1613 3905 5711 1613 Size: 2292 1806 4666 Fragments arranged by size: 4666 2292 1806 PsiI TTA'TAA Cuts at: 6334 6334 Size: 8764 PstI C_TGCA'G Cuts at: 830 2331 2364 2466 6018 6116 830 Size: 1501 33 102 3552 98 3478 Fragments arranged by size: 3552 3478 1501 102 98 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8764 PvuII CAG'CTG Cuts at: 2365 2461 2893 3374 3946 4861 2365 Size: 96 432 481 572 915 6268 Fragments arranged by size: 6268 915 572 481 432 96 RsrII CG'GwC_CG Cuts at: 5827 5827 Size: 8764 SacI G_AGCT'C Cuts at: 2711 3985 2711 Size: 1274 7490 SacII CC_GC'GG Cuts at: 2759 2759 Size: 8764 SalI G'TCGA_C Cuts at: 1936 4416 6106 1936 Size: 2480 1690 4594 Fragments arranged by size: 4594 2480 1690 SapI GCTCTTCn'nnn_ Cuts at: 2564 2564 Size: 8764 SbfI CC_TGCA'GG Cuts at: 6116 6116 Size: 8764 ScaI AGT'ACT Cuts at: 7012 8724 7012 Size: 1712 7052 SexAI A'CCwGG_T Cuts at: 5816 5816 Size: 8764 SfcI C'TryA_G Cuts at: 826 1045 2327 2360 2462 3296 4255 5893 6014 Size: 219 1282 33 102 834 959 1638 121 Cuts at: 6014 6112 7247 7928 8119 826 Size: 98 1135 681 191 1471 Fragments arranged by size: 1638 1471 1282 1135 959 834 681 219 191 121 102 98 33 SfoI GGC'GCC Cuts at: 2161 2167 4228 2161 Size: 6 2061 6697 Fragments arranged by size: 6697 2061 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 SgrAI Cr'CCGG_yG Cuts at: 1700 2162 1700 Size: 462 8302 SmaI CCC'GGG Cuts at: 1872 2627 5680 6091 1872 Size: 755 3053 411 4545 Fragments arranged by size: 4545 3053 755 411 SmlI C'TyrA_G Cuts at: 820 1017 1943 6868 7769 8031 820 Size: 197 926 4925 901 262 1553 Fragments arranged by size: 4925 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6555 493 Size: 6062 2702 SpeI A'CTAG_T Cuts at: 152 4666 152 Size: 4514 4250 SphI G_CATG'C Cuts at: 1132 3076 4769 6122 1132 Size: 1944 1693 1353 3774 Fragments arranged by size: 3774 1944 1693 1353 SspI AAT'ATT Cuts at: 5 52 6688 5 Size: 47 6636 2081 Fragments arranged by size: 6636 2081 47 StuI AGG'CCT Cuts at: 1517 3231 4297 1517 Size: 1714 1066 5984 Fragments arranged by size: 5984 1714 1066 StyI C'CwwG_G Cuts at: 513 1065 3226 3924 4285 6203 513 Size: 552 2161 698 361 1918 3074 Fragments arranged by size: 3074 2161 1918 698 552 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 3353 6791 6808 6961 7146 7591 Size: 1255 591 1182 3438 17 153 185 445 Cuts at: 7591 325 Size: 1498 Fragments arranged by size: 3438 1498 1255 1182 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3018 3657 4135 Size: 274 80 33 51 1443 1041 639 478 Cuts at: 4135 5760 7010 8722 96 Size: 1625 1250 1712 138 Fragments arranged by size: 1712 1625 1443 1250 1041 639 478 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 3116 3148 3792 3824 8724 8756 2163 Size: 32 921 32 644 32 4900 32 2171 Fragments arranged by size: 4900 2171 921 644 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8764 XbaI T'CTAG_A Cuts at: 6100 6100 Size: 8764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2843 3142 2287 Size: 556 299 7909 Fragments arranged by size: 7909 556 299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8764 XmaI C'CCGG_G Cuts at: 1870 2625 5678 6089 1870 Size: 755 3053 411 4545 Fragments arranged by size: 4545 3053 755 411 XmnI GAAnn'nnTTC Cuts at: 3389 4680 6893 3389 Size: 1291 2213 5260 Fragments arranged by size: 5260 2213 1291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2945 6572 276 Size: 53 83 186 936 1411 3627 2468 Fragments arranged by size: 3627 2468 1411 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI SanDI SfiI SrfI SwaI