Restriction map for
pFN21AA1121
(Linear) (Six-base) MAPSORT of: KIAA1121.seq Check: 1427 from: 1 to: 4059
KIAA1121.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2924 4059
Size: 2924 1135
AatII G_ACGT'C
Cuts at: 0 942 4059
Size: 942 3117
AccI GT'mk_AC
Cuts at: 0 942 2412 2792 4059
Size: 942 1470 380 1267
Fragments arranged by size:
1470 1267 942 380
AclI AA'CG_TT
Cuts at: 0 2331 4059
Size: 2331 1728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1048 2438 2662 3205 3272 3307 4059
Size: 583 465 1390 224 543 67 35 752
Fragments arranged by size:
1390 752 583 543 465 224 67 35
AflIII A'CryG_T
Cuts at: 0 1010 1655 2709 4059
Size: 1010 645 1054 1350
Fragments arranged by size:
1350 1054 1010 645
AgeI A'CCGG_T
Cuts at: 0 473 4059
Size: 473 3586
AhdI GACnn_n'nnGTC
Cuts at: 0 12 4059
Size: 12 4047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 82 4059
Size: 82 3977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 851 883 1150 1182 4059
Size: 851 32 267 32 2877
Fragments arranged by size:
2877 851 267 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 456 2859 4059
Size: 456 2403 1200
Fragments arranged by size:
2403 1200 456
ApoI r'AATT_y
Cuts at: 0 718 976 1999 2902 4059
Size: 718 258 1023 903 1157
Fragments arranged by size:
1157 1023 903 718 258
AvaI C'yCGr_G
Cuts at: 0 620 3673 4059
Size: 620 3053 386
Fragments arranged by size:
3053 620 386
BanI G'GyrC_C
Cuts at: 0 154 160 1330 2221 3138 3184 4059
Size: 154 6 1170 891 917 46 875
Fragments arranged by size:
1170 917 891 875 154 46 6
BanII G_rGCy'C
Cuts at: 0 124 706 1980 4059
Size: 124 582 1274 2079
Fragments arranged by size:
2079 1274 582 124
BbeI G_GCGC'C
Cuts at: 0 158 164 2225 4059
Size: 158 6 2061 1834
Fragments arranged by size:
2061 1834 158 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 621 4049 4058 4059
Size: 621 3428 9 1
Fragments arranged by size:
3428 621 9 1
BbvCI CC'TCA_GC
Cuts at: 0 682 1937 4059
Size: 682 1255 2122
Fragments arranged by size:
2122 1255 682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2165 2199 4059
Size: 2165 34 1860
Fragments arranged by size:
2165 1860 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1002 1086 4059
Size: 1002 84 2973
Fragments arranged by size:
2973 1002 84
BclI T'GATC_A
Cuts at: 0 1299 3774 4059
Size: 1299 2475 285
Fragments arranged by size:
2475 1299 285
BglI GCCn_nnn'nGGC
Cuts at: 0 251 4059
Size: 251 3808
BglII A'GATC_T
Cuts at: 0 1518 3195 4059
Size: 1518 1677 864
Fragments arranged by size:
1677 1518 864
BlpI GC'TnA_GC
Cuts at: 0 700 4059
Size: 700 3359
Bme1580I G_kGCm'C
Cuts at: 0 1333 1892 4059
Size: 1333 559 2167
Fragments arranged by size:
2167 1333 559
BmgBI CAC'GTC
Cuts at: 0 3684 4059
Size: 3684 375
BmrI ACTGGGnnnn_n'
Cuts at: 0 2476 4059
Size: 2476 1583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 1084 2807 4059
Size: 252 832 1723 1252
Fragments arranged by size:
1723 1252 832 252
Bpu10I CC'TnA_GC
Cuts at: 0 682 1937 4059
Size: 682 1255 2122
Fragments arranged by size:
2122 1255 682
BsaAI yAC'GTr
Cuts at: 0 3699 4059
Size: 3699 360
BsaHI Gr'CG_yC
Cuts at: 0 155 161 939 1403 2222 3215 3692 4059
Size: 155 6 778 464 819 993 477 367
Fragments arranged by size:
993 819 778 477 464 367 155 6
BsaWI w'CCGG_w
Cuts at: 0 473 3824 3953 4059
Size: 473 3351 129 106
Fragments arranged by size:
3351 473 129 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 851 881 1015 1045 1150 1180
Size: 158 30 663 30 134 30 105 30
Cuts at: 1180 1787 1817 1916 1946 3775 3805 4059
Size: 607 30 99 30 1829 30 254
Fragments arranged by size:
1829 663 607 254 158 134 105 99
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 64 346 349 352 1036 1162 1295
Size: 61 3 282 3 3 684 126 133
Cuts at: 1295 2509 2704 3763 4059
Size: 1214 195 1059 296
Fragments arranged by size:
1214 1059 684 296 282 195 133 126
61 3 3 3
BseYI C'CCAG_C
Cuts at: 0 103 141 238 274 1745 4059
Size: 103 38 97 36 1471 2314
Fragments arranged by size:
2314 1471 103 97 38 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 559 1660 1689 3226 3792 4059
Size: 200 359 1101 29 1537 566 267
Fragments arranged by size:
1537 1101 566 359 267 200 29
BsiEI CG_ry'CG
Cuts at: 0 128 261 573 4059
Size: 128 133 312 3486
Fragments arranged by size:
3486 312 133 128
BsiHKAI G_wGCw'C
Cuts at: 0 71 706 1399 1980 2696 4059
Size: 71 635 693 581 716 1363
Fragments arranged by size:
1363 716 693 635 581 71
BsmI GAATG_Cn'
Cuts at: 0 1569 4059
Size: 1569 2490
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2761 3967 4059
Size: 2761 1206 92
Fragments arranged by size:
2761 1206 92
Bsp1286I G_dGCh'C
Cuts at: 0 71 124 706 1333 1399 1892 1980 2696
Size: 71 53 582 627 66 493 88 716
Cuts at: 2696 4059
Size: 1363
Fragments arranged by size:
1363 716 627 582 493 88 71 66
53
BspEI T'CCGG_A
Cuts at: 0 3953 4059
Size: 3953 106
BspHI T'CATG_A
Cuts at: 0 527 4059
Size: 527 3532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 1632 2924 3248 4059
Size: 1052 580 1292 324 811
Fragments arranged by size:
1292 1052 811 580 324
BsrBI CCG'CTC
Cuts at: 0 56 318 3003 4059
Size: 56 262 2685 1056
Fragments arranged by size:
2685 1056 262 56
BsrDI GCAATG_nn'
Cuts at: 0 2901 4059
Size: 2901 1158
BsrFI r'CCGG_y
Cuts at: 0 127 157 260 428 473 1071 2609 4059
Size: 127 30 103 168 45 598 1538 1450
Fragments arranged by size:
1538 1450 598 168 127 103 45 30
BsrGI T'GTAC_A
Cuts at: 0 1013 3755 4059
Size: 1013 2742 304
Fragments arranged by size:
2742 1013 304
BssHII G'CGCG_C
Cuts at: 0 89 91 4059
Size: 89 2 3968
Fragments arranged by size:
3968 89 2
BstBI TT'CG_AA
Cuts at: 0 857 4059
Size: 857 3202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 1002 2281 2831 3657 4059
Size: 420 582 1279 550 826 402
Fragments arranged by size:
1279 826 582 550 420 402
BstYI r'GATC_y
Cuts at: 0 1518 2955 3195 4059
Size: 1518 1437 240 864
Fragments arranged by size:
1518 1437 864 240
Bsu36I CC'TnA_GG
Cuts at: 0 1697 4059
Size: 1697 2362
BtgI C'CryG_G
Cuts at: 0 735 751 1865 2280 4059
Size: 735 16 1114 415 1779
Fragments arranged by size:
1779 1114 735 415 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 369 4049 4059
Size: 369 3680 10
Fragments arranged by size:
3680 369 10
BtsI GCAGTG_nn'
Cuts at: 0 1360 4059
Size: 1360 2699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1253 1288 4059
Size: 1253 35 2771
Fragments arranged by size:
2771 1253 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2775 4059
Size: 2775 1284
EaeI y'GGCC_r
Cuts at: 0 125 258 713 1052 1764 2118 3686 4059
Size: 125 133 455 339 712 354 1568 373
Fragments arranged by size:
1568 712 455 373 354 339 133 125
EagI C'GGCC_G
Cuts at: 0 125 258 4059
Size: 125 133 3801
Fragments arranged by size:
3801 133 125
EarI CTCTTCn'nnn_
Cuts at: 0 302 343 559 643 737 1603 2013 2573
Size: 302 41 216 84 94 866 410 560
Cuts at: 2573 3251 4059
Size: 678 808
Fragments arranged by size:
866 808 678 560 410 302 216 94
84 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 597 3700 4059
Size: 597 3103 359
Fragments arranged by size:
3103 597 359
EcoICRI GAG'CTC
Cuts at: 0 704 1978 4059
Size: 704 1274 2081
Fragments arranged by size:
2081 1274 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 583 1048 1084 2438 2662 2807 3205
Size: 252 331 465 36 1354 224 145 398
Cuts at: 3205 3272 3307 4059
Size: 67 35 752
Fragments arranged by size:
1354 752 465 398 331 252 224 145
67 36 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 4059
Size: 685 3374
EcoO109I rG'GnC_Cy
Cuts at: 0 747 3237 3912 4059
Size: 747 2490 675 147
Fragments arranged by size:
2490 747 675 147
FseI GG_CCGG'CC
Cuts at: 0 264 4059
Size: 264 3795
FspI TGC'GCA
Cuts at: 0 658 4059
Size: 658 3401
HaeII r_GCGC'y
Cuts at: 0 137 158 164 2225 4059
Size: 137 21 6 2061 1834
Fragments arranged by size:
2061 1834 137 21 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1015 1047 2992 3024 3163 3195 4059
Size: 30 985 32 1945 32 139 32 864
Fragments arranged by size:
1945 985 864 139 32 32 32 30
HincII GTy'rAC
Cuts at: 0 1041 2413 2544 3099 3818 4059
Size: 1041 1372 131 555 719 241
Fragments arranged by size:
1372 1041 719 555 241 131
HindIII A'AGCT_T
Cuts at: 0 2632 4059
Size: 2632 1427
HpaI GTT'AAC
Cuts at: 0 3099 4059
Size: 3099 960
KasI G'GCGC_C
Cuts at: 0 154 160 2221 4059
Size: 154 6 2061 1838
Fragments arranged by size:
2061 1838 154 6
MfeI C'AATT_G
Cuts at: 0 1711 2972 4059
Size: 1711 1261 1087
Fragments arranged by size:
1711 1261 1087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1394 2036 4059
Size: 293 1101 642 2023
Fragments arranged by size:
2023 1101 642 293
MscI TGG'CCA
Cuts at: 0 715 1054 2120 4059
Size: 715 339 1066 1939
Fragments arranged by size:
1939 1066 715 339
MslI CAynn'nnrTG
Cuts at: 0 418 1138 2279 3144 3463 3799 4021 4059
Size: 418 720 1141 865 319 336 222 38
Fragments arranged by size:
1141 865 720 418 336 319 222 38
MspA1I CmG'CkG
Cuts at: 0 200 212 360 456 753 888 1369 1941
Size: 200 12 148 96 297 135 481 572
Cuts at: 1941 2856 4059
Size: 915 1203
Fragments arranged by size:
1203 915 572 481 297 200 148 135
96 12
NaeI GCC'GGC
Cuts at: 0 129 159 262 430 4059
Size: 129 30 103 168 3629
Fragments arranged by size:
3629 168 129 103 30
NarI GG'CG_CC
Cuts at: 0 155 161 2222 4059
Size: 155 6 2061 1837
Fragments arranged by size:
2061 1837 155 6
NcoI C'CATG_G
Cuts at: 0 2280 4059
Size: 2280 1779
NgoMIV G'CCGG_C
Cuts at: 0 127 157 260 428 4059
Size: 127 30 103 168 3631
Fragments arranged by size:
3631 168 127 103 30
NspI r_CATG'y
Cuts at: 0 1014 1071 2713 2764 4059
Size: 1014 57 1642 51 1295
Fragments arranged by size:
1642 1295 1014 57 51
PasI CC'CwG_GG
Cuts at: 0 1335 4059
Size: 1335 2724
PciI A'CATG_T
Cuts at: 0 1010 2709 4059
Size: 1010 1699 1350
Fragments arranged by size:
1699 1350 1010
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 4059
Size: 510 3549
PfoI T'CCnGG_A
Cuts at: 0 706 3914 4059
Size: 706 3208 145
Fragments arranged by size:
3208 706 145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 851 883 1150 1182 4059
Size: 851 32 267 32 2877
Fragments arranged by size:
2877 851 267 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 747 3237 3912 4059
Size: 747 2490 675 147
Fragments arranged by size:
2490 747 675 147
PshAI GACnn'nnGTC
Cuts at: 0 1900 3706 4059
Size: 1900 1806 353
Fragments arranged by size:
1900 1806 353
PstI C_TGCA'G
Cuts at: 0 326 359 461 4013 4059
Size: 326 33 102 3552 46
Fragments arranged by size:
3552 326 102 46 33
PvuII CAG'CTG
Cuts at: 0 360 456 888 1369 1941 2856 4059
Size: 360 96 432 481 572 915 1203
Fragments arranged by size:
1203 915 572 481 432 360 96
RsrII CG'GwC_CG
Cuts at: 0 3822 4059
Size: 3822 237
SacI G_AGCT'C
Cuts at: 0 706 1980 4059
Size: 706 1274 2079
Fragments arranged by size:
2079 1274 706
SacII CC_GC'GG
Cuts at: 0 754 4059
Size: 754 3305
SalI G'TCGA_C
Cuts at: 0 2411 4059
Size: 2411 1648
SapI GCTCTTCn'nnn_
Cuts at: 0 559 4059
Size: 559 3500
SexAI A'CCwGG_T
Cuts at: 0 3811 4059
Size: 3811 248
SfcI C'TryA_G
Cuts at: 0 322 355 457 1291 2250 3888 4009 4059
Size: 322 33 102 834 959 1638 121 50
Fragments arranged by size:
1638 959 834 322 121 102 50 33
SfoI GGC'GCC
Cuts at: 0 156 162 2223 4059
Size: 156 6 2061 1836
Fragments arranged by size:
2061 1836 156 6
SgrAI Cr'CCGG_yG
Cuts at: 0 157 4059
Size: 157 3902
SmaI CCC'GGG
Cuts at: 0 622 3675 4059
Size: 622 3053 384
Fragments arranged by size:
3053 622 384
SpeI A'CTAG_T
Cuts at: 0 2661 4059
Size: 2661 1398
SphI G_CATG'C
Cuts at: 0 1071 2764 4059
Size: 1071 1693 1295
Fragments arranged by size:
1693 1295 1071
StuI AGG'CCT
Cuts at: 0 1226 2292 4059
Size: 1226 1066 1767
Fragments arranged by size:
1767 1226 1066
StyI C'CwwG_G
Cuts at: 0 1221 1919 2280 4059
Size: 1221 698 361 1779
Fragments arranged by size:
1779 1221 698 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 1348 4059
Size: 166 1182 2711
Fragments arranged by size:
2711 1182 166
TatI w'GTAC_w
Cuts at: 0 1013 1652 2130 3755 4059
Size: 1013 639 478 1625 304
Fragments arranged by size:
1625 1013 639 478 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1111 1143 1787 1819 4059
Size: 158 32 921 32 644 32 2240
Fragments arranged by size:
2240 921 644 158 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 838 1137 4059
Size: 282 556 299 2922
Fragments arranged by size:
2922 556 299 282
XmaI C'CCGG_G
Cuts at: 0 620 3673 4059
Size: 620 3053 386
Fragments arranged by size:
3053 620 386
XmnI GAAnn'nnTTC
Cuts at: 0 1384 2675 4059
Size: 1384 1291 1384
Fragments arranged by size:
1384 1384 1291
ZraI GAC'GTC
Cuts at: 0 940 4059
Size: 940 3119
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII RsrII SacI SacII
SalI SapI SexAI SfcI SfoI SgrAI SmaI SpeI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AleI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BfrBI BmtI BplI BpuEI
BsaI BsaBI BsiWI BssSI BstAPI BstEII BstZ17I ClaI
DraI DraIII EcoRI EcoRV FalI FspAI KpnI MluI
NdeI NheI NotI NruI NsiI PacI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI SanDI SbfI ScaI
SfiI SgfI SmlI SnaBI SrfI SspI SwaI Tth111I
XbaI XhoI