Restriction map for
pFN21AE0163
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI AlwI AlwNI ApaI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I EarI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI Ksp632I MaeI
MaeII MamI MboI MboII MfeI
MflI MluI MluNI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 77 enzymes) #
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Acc65I 107 GGTACC G^GTACC
AccIII 218 TCCGGA T^CCGGA
AcsI 244 GAATTT [GA]^AATT[CT]
AfaI 108 GTAC GT^AC
AflIII 225 ACATGT A^C[GA][CT]GT
Alw44I 128 GTGCAC G^TGCAC
AocI 8 CCTTAGG CC^T[ACGT]AGG
Aor51HI 263 AGCGCT AGC^GCT
ApaLI 128 GTGCAC G^TGCAC
ApoI 244 GAATTT [GA]^AATT[CT]
Asp700I 117 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 107 GGTACC G^GTACC
AspHI 128 GTGCAC G[AT]GC[AT]^C
AvaII 14 GGTCC G^G[AT]CC
BanI 107 GGTACC G^G[CT][GA]CC
BanII 161 GAGCCC G[GA]GC[CT]^C
BbvI 187 GCTGC GCAGC(8/12)
BcgI 24 GCACACTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 18 CCCGG CC^[GC]GG
BpmI 317 CTGGAG CTGGAG(16/14)
BsaI 295 GGTCTC GGTCTC(1/5)
BsaWI 218 TCCGGA [AT]^CCGG[AT]
BseAI 218 TCCGGA T^CCGGA
BseRI 274 GAGGAG GAGGAG(10/8)
BsgI 156 GTGCAG GTGCAG(16/14)
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsiHKAI 128 GTGCAC G[AT]GC[AT]^C
BsmAI 296 GTCTC GTCTC(1/5)
BsmFI 15 GTCCC GGGAC(10/14)
BspEI 218 TCCGGA T^CCGGA
BspLU11I 225 ACATGT A^CATGT
BsrFI 73 ACCGGC [GA]^CCGG[CT]
BsrI 143 CCAGT ACTGG(1/-1)
BstEII 138 GGTCACC G^GT[ACGT]ACC
BstF5I 216 CATCC GGATG(2/0)
BstNI 316 CCTGG CC^[AT]GG
BstPI 138 GGTCACC G^GT[ACGT]ACC
BstXI 309 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 8 CCTTAGG CC^T[ACGT]AGG
Cac8I 283 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 73 ACCGGC [GA]^CCGG[CT]
DraII 13 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 95 CCGCGG C^C[GA][CT]GG
EagI 75 CGGCCG C^GGCCG
EclXI 75 CGGCCG C^GGCCG
Eco47III 263 AGCGCT AGC^GCT
Eco52I 75 CGGCCG C^GGCCG
Eco81I 8 CCTTAGG CC^T[ACGT]AGG
EcoO109I 13 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 138 GGTCACC G^GT[ACGT]ACC
EcoRII 316 CCTGG ^CC[AT]GG
FauI 45 GCGGG CCCGC(4/6)
FokI 216 CATCC GGATG(9/13)
HaeII 263 AGCGCT [GA]GCGC^[CT]
HinfI 277 GAGTC G^A[ACGT]TC
KpnI 107 GGTACC GGTAC^C
KspI 95 CCGCGG CCGC^GG
MaeIII 139 GTCAC ^GT[ACGT]AC
MroI 218 TCCGGA T^CCGGA
MspA1I 95 CCGCGG C[AC]G^C[GT]G
MvaI 316 CCTGG CC^[AT]GG
NciI 18 CCCGG CC^[GC]GG
NruI 87 TCGCGA TCG^CGA
NspI 225 ACATGT [GA]CATG^[CT]
PleI 277 GAGTC GAGTC(4/5)
PpuMI 13 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 108 GTAC GT^AC
SacII 95 CCGCGG CCGC^GG
SfaNI 215 GCATC GCATC(5/9)
SmlI 298 CTCAAG C^T[CT][GA]AG
TaqI 33 TCGA T^CGA
TseI 187 GCTGC G^C[AT]GC
Tsp45I 139 GTCAC ^GT[GC]AC
Tsp509I 245 AATT ^AATT
TspRI 144 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 33 TCGA T^CGA
XmnI 117 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 173 AGCT AG^CT
186 AGCT
BmyI 128 GTGCAC G[AGT]GC[ACT]^C
161 GAGCCC
BsaJI 17 CCCCGG C^C[ACGT][ACGT]GG
95 CCGCGG
Bsp1286I 128 GTGCAC G[AGT]GC[ACT]^C
161 GAGCCC
BssKI 18 CCCGG ^CC[ACGT]GG
316 CCTGG
Cfr13I 14 GGTCC G^G[ACGT]CC
231 GGCCC
DdeI 9 CTTAG C^T[ACGT]AG
61 CTGAG
EaeI 68 CGGCCA [CT]^GGCC[GA]
75 CGGCCG
EcoNI 111 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
234 CCTACATCAGG
HphI 259 GGTGA GGTGA(8/7)
140 TCACC
Sau96I 14 GGTCC G^G[ACGT]CC
231 GGCCC
ScrFI 18 CCCGG CC^[ACGT]GG
316 CCTGG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AccII AciI BsiYI BslI BstUI
CfoI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvnI MwoI NlaIII NlaIV