(Circular) (Six-base) MAPSORT of: pFN21AE0178.seq Check: 1903 from: 1 to: 5746 pFN21AE0178 5746 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2766 2766 Size: 5746 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2961 3089 1937 Size: 1024 128 4594 Fragments arranged by size: 4594 1024 128 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 2655 3809 5193 5461 1632 Size: 884 139 1154 1384 268 1917 Fragments arranged by size: 1917 1384 1154 884 268 139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 2349 4645 5622 1203 Size: 1146 2296 977 1327 Fragments arranged by size: 2296 1327 1146 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 2054 2131 2813 3489 5061 2054 Size: 77 682 676 1572 2739 Fragments arranged by size: 2739 1572 682 676 77 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 2114 2528 3055 3281 3433 5542 1493 Size: 621 414 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 621 527 414 226 152 AseI AT'TA_AT Cuts at: 160 1032 2213 4300 160 Size: 872 1181 2087 1606 Fragments arranged by size: 2087 1606 1181 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 3071 1870 Size: 73 1128 4545 Fragments arranged by size: 4545 1128 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2287 2457 3065 1391 Size: 896 170 608 4072 Fragments arranged by size: 4072 896 608 170 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 2520 2914 928 Size: 971 498 123 394 3760 Fragments arranged by size: 3760 971 498 394 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2178 3638 4848 2178 Size: 1460 1210 3076 Fragments arranged by size: 3076 1460 1210 BclI T'GATC_A Cuts at: 1548 2553 1548 Size: 1005 4741 BfrBI ATG'CAT Cuts at: 2833 2833 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2485 3807 4963 1790 Size: 695 1322 1156 2573 Fragments arranged by size: 2573 1322 1156 695 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2724 2724 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 4406 1239 Size: 1062 2105 2579 Fragments arranged by size: 2579 2105 1062 Bpu10I CC'TnA_GC Cuts at: 2265 2670 2265 Size: 405 5341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2146 2698 4409 882 Size: 617 647 552 1711 2219 Fragments arranged by size: 2219 1711 647 617 552 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2517 3553 3935 4570 Size: 53 83 186 936 984 1036 382 635 Cuts at: 4570 275 Size: 1451 Fragments arranged by size: 1451 1036 984 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 1178 Size: 30 165 30 1374 30 4117 Fragments arranged by size: 4117 1374 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2716 1186 Size: 1530 4216 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2455 1163 Size: 573 719 4454 Fragments arranged by size: 4454 719 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2287 2795 3807 3892 4963 2287 Size: 508 1012 85 1071 3070 Fragments arranged by size: 3070 1071 1012 508 85 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2457 2485 2795 3065 3807 3892 Size: 399 497 170 28 310 270 742 85 Cuts at: 3892 4963 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 497 399 310 270 170 85 28 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2766 3101 844 Size: 1922 335 3489 Fragments arranged by size: 3489 1922 335 BsrBI CCG'CTC Cuts at: 1394 1823 2674 3633 1394 Size: 429 851 959 3507 Fragments arranged by size: 3507 959 851 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 4241 4415 65 Size: 1183 1086 1907 174 1396 Fragments arranged by size: 1907 1396 1183 1086 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 4390 4577 1700 Size: 110 265 2315 187 2869 Fragments arranged by size: 2869 2315 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 2839 794 Size: 2045 3701 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2087 2087 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2008 3076 3443 3828 3845 5297 5383 5395 Size: 36 1068 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1068 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2613 2613 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 2004 2696 513 Size: 552 939 692 3563 Fragments arranged by size: 3563 939 692 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3036 487 Size: 683 56 1810 3197 Fragments arranged by size: 3197 1810 683 56 BtsI GCAGTG_nn' Cuts at: 2223 3262 4056 4076 2223 Size: 1039 794 20 3893 Fragments arranged by size: 3893 1039 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 2784 3050 2784 Size: 266 5480 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2925 3193 Size: 54 1425 50 275 18 247 848 268 Cuts at: 3193 4082 5711 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 848 275 268 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3676 1353 Size: 1224 1099 3423 Fragments arranged by size: 3423 1224 1099 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2285 2285 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2516 2655 3809 4406 5193 5461 Size: 393 669 215 139 1154 597 787 268 Cuts at: 5461 1239 Size: 1524 Fragments arranged by size: 1524 1154 787 669 597 393 268 215 139 EcoNI CCTnn'n_nnAGG Cuts at: 2576 2576 Size: 5746 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 EcoRV GAT'ATC Cuts at: 2950 2950 Size: 5746 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2812 2812 Size: 5746 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2299 2662 4893 1678 Size: 621 363 2231 2531 Fragments arranged by size: 2531 2231 621 363 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2527 2559 4387 Size: 32 135 32 493 32 430 32 1828 Cuts at: 4387 4419 4461 4493 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1828 493 430 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2020 2962 3090 3336 669 Size: 988 281 82 942 128 246 3079 Fragments arranged by size: 3079 988 942 281 246 128 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 2106 3345 2106 Size: 1239 4507 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4860 5044 1305 Size: 1251 2304 184 2007 Fragments arranged by size: 2304 2007 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2927 10 Size: 54 1425 343 1095 2829 Fragments arranged by size: 2829 1425 1095 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2382 3704 4063 4222 518 Size: 648 919 297 1322 359 159 2042 Fragments arranged by size: 2042 1322 919 648 359 297 159 MspA1I CmG'CkG Cuts at: 1606 2128 2865 3162 3839 4987 5232 1606 Size: 522 737 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 522 297 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2696 513 Size: 552 939 692 3563 Fragments arranged by size: 3563 939 692 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NsiI A_TGCA'T Cuts at: 2835 2835 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2095 2353 3104 4649 1132 Size: 97 866 258 751 1545 2229 Fragments arranged by size: 2229 1545 866 751 258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 2349 4645 2349 Size: 2296 3450 PflMI CCAn_nnn'nTGG Cuts at: 1108 2548 2655 2973 3010 1108 Size: 1440 107 318 37 3844 Fragments arranged by size: 3844 1440 318 107 37 PfoI T'CCnGG_A Cuts at: 2508 2508 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2663 2695 3815 3847 5328 5360 2663 Size: 32 1120 32 1481 32 3049 Fragments arranged by size: 3049 1481 1120 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2128 2128 Size: 5746 SacI G_AGCT'C Cuts at: 2287 2287 Size: 5746 SalI G'TCGA_C Cuts at: 1936 2960 3088 1936 Size: 1024 128 4594 Fragments arranged by size: 4594 1024 128 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 2522 2696 3185 513 Size: 552 939 201 317 174 489 3074 Fragments arranged by size: 3074 939 552 489 317 201 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2820 2984 3773 3790 3943 4128 4573 Size: 1255 1240 164 789 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 1240 789 445 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3992 5704 Size: 274 80 33 51 1443 369 1646 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1646 1443 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 2913 1316 Size: 1597 4149 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 2586 3875 2586 Size: 1289 4457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI