Restriction map for
pFN21AE0153
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstF5I BstNI
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT22I
FbaI FokI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
HphI KasI KspI MaeI MaeII
MfeI MluI MroI MslI MspA1I
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 710 GGTACC G^GTACC
AlwNI 731 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 710 GGTACC G^GTACC
AspHI 718 GAGCTC G[AT]GC[AT]^C
AvaII 244 GGTCC G^G[AT]CC
BalI 927 TGGCCA TGG^CCA
BanI 710 GGTACC G^G[CT][GA]CC
BanII 718 GAGCTC G[GA]GC[CT]^C
BcnI 28 CCCGG CC^[GC]GG
BmyI 718 GAGCTC G[AGT]GC[ACT]^C
BsaBI 154 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 225 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 718 GAGCTC G[AT]GC[AT]^C
Bsp1286I 718 GAGCTC G[AGT]GC[ACT]^C
BssKI 28 CCCGG ^CC[ACGT]GG
BstBI 110 TTCGAA TT^CGAA
BstXI 587 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 885 GGATCT [GA]^GATC[CT]
CfoI 21 GCGC GCG^C
EaeI 927 TGGCCA [CT]^GGCC[GA]
EarI 960 GAAGAG CTCTTC(1/4)
EcoRV 253 GATATC GAT^ATC
EcoT14I 225 CCTTGG C^C[AT][AT]GG
HapII 29 CCGG C^CGG
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HpaII 29 CCGG C^CGG
KpnI 710 GGTACC GGTAC^C
Ksp632I 960 GAAGAG CTCTTC(1/4)
MamI 154 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 885 GGATCT [GA]^GATC[CT]
MluNI 927 TGGCCA TGG^CCA
MscI 927 TGGCCA TGG^CCA
MspI 29 CCGG C^CGG
NciI 28 CCCGG CC^[GC]GG
NspV 110 TTCGAA TT^CGAA
PstI 488 CTGCAG CTGCA^G
SacI 718 GAGCTC GAGCT^C
ScrFI 28 CCCGG CC^[ACGT]GG
SfcI 488 CTGCAG C^T[GA][CT]AG
SfuI 110 TTCGAA TT^CGAA
SmlI 742 CTCAAG C^T[CT][GA]AG
StuI 294 AGGCCT AGG^CCT
StyI 225 CCTTGG C^C[AT][AT]GG
TaqI 111 TCGA T^CGA
TthHB8I 111 TCGA T^CGA
XhoII 885 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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BpmI 99 CTCCAG CTGGAG(16/14)
315 CTGGAG
BseRI 784 GAGGAG GAGGAG(10/8)
988 GAGGAG
BsgI 671 GTGCAG GTGCAG(16/14)
832 GTGCAG
BsmAI 789 GAGAC GTCTC(1/5)
97 GTCTC
DdeI 338 CTGAG C^T[ACGT]AG
374 CTCAG
FauI 14 GCGGG CCCGC(4/6)
32 GCGGG
HindIII 136 AAGCTT A^AGCTT
797 AAGCTT
MaeIII 104 GTGAC ^GT[ACGT]AC
652 GTGAC
MseI 305 TTAA T^TAA
518 TTAA
MwoI 14 GCGGGAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
23 GCAGGCCCGGC
NlaIV 39 GGGGCC GG[ACGT]^[ACGT]CC
710 GGTACC
SfaNI 733 GATGC GCATC(5/9)
993 GCATC
Tru9I 305 TTAA T^TAA
518 TTAA
Tsp45I 104 GTGAC ^GT[GC]AC
652 GTGAC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsrI Cac8I Cfr13I
DpnII Fnu4HI HaeIII HinfI ItaI
MboI MboII MnlI NdeII NlaIII
RsaI Sau3AI Sau96I TfiI TseI
Tsp509I TspRI