(Circular) (Six-base) MAPSORT of: pFN21AE0140.seq Check: 8163 from: 1 to: 5560 pFN21AE0140 5560 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 2903 1937 Size: 541 425 4594 Fragments arranged by size: 4594 541 425 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3623 5007 5275 1632 Size: 1185 806 1384 268 1917 Fragments arranged by size: 1917 1384 1185 806 268 AflII C'TTAA_G Cuts at: 820 1017 2497 820 Size: 197 1480 3883 Fragments arranged by size: 3883 1480 197 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 2349 4391 2349 Size: 2042 3518 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 2522 3303 4875 2522 Size: 781 1572 3207 Fragments arranged by size: 3207 1572 781 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2211 2337 2869 3095 3247 5356 1493 Size: 718 126 532 226 152 2109 1697 Fragments arranged by size: 2109 1697 718 532 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2885 1870 Size: 73 121 821 4545 Fragments arranged by size: 4545 821 121 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2023 2881 4336 618 Size: 325 217 627 224 12 858 1455 1842 Fragments arranged by size: 1842 1455 858 627 325 224 217 12 BanII G_rGCy'C Cuts at: 1391 2148 2879 1391 Size: 757 731 4072 Fragments arranged by size: 4072 757 731 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2562 4171 136 Size: 107 122 71 631 605 890 1609 1525 Fragments arranged by size: 1609 1525 890 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 2603 3621 4777 1790 Size: 813 1018 1156 2573 Fragments arranged by size: 2573 1156 1018 813 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4220 1239 Size: 2981 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 3685 4550 4848 2129 Size: 1556 865 298 2841 Fragments arranged by size: 2841 1556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3367 3749 4384 275 Size: 53 83 186 936 1834 382 635 1451 Fragments arranged by size: 1834 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2349 3993 4391 4665 4812 5223 2349 Size: 1644 398 274 147 411 2686 Fragments arranged by size: 2686 1644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5335 Fragments arranged by size: 5335 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4620 BseYI C'CCAG_C Cuts at: 2268 2415 2596 4763 5259 2268 Size: 147 181 2167 496 2569 Fragments arranged by size: 2569 2167 496 181 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2355 1163 Size: 573 619 4368 Fragments arranged by size: 4368 619 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 3621 3706 4777 2739 Size: 882 85 1071 3522 Fragments arranged by size: 3522 1071 882 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2603 2739 2879 3621 3706 4777 Size: 399 358 455 136 140 742 85 1071 Cuts at: 4777 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 455 399 358 140 136 85 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2915 844 Size: 2071 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3447 1394 Size: 429 1624 3507 Fragments arranged by size: 3507 1624 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2349 4204 4391 1700 Size: 110 210 329 1855 187 2869 Fragments arranged by size: 2869 1855 329 210 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 2388 3620 4632 2388 Size: 1232 1012 3316 Fragments arranged by size: 3316 1232 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2378 794 Size: 1584 3976 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555 Size: 918 367 385 17 1452 86 12 346 Cuts at: 5555 1972 Size: 1977 Fragments arranged by size: 1977 1452 918 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2479 2479 Size: 5560 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 513 Size: 552 939 23 4046 Fragments arranged by size: 4046 939 552 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 2439 2807 3076 3870 3890 2439 Size: 368 269 794 20 4109 Fragments arranged by size: 4109 794 368 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DraIII CAC_nnn'GTG Cuts at: 2378 2378 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525 Size: 54 1425 50 275 18 1177 889 1629 Cuts at: 5525 8 Size: 43 Fragments arranged by size: 1629 1425 1177 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2700 3490 1353 Size: 1347 790 3423 Fragments arranged by size: 3423 1347 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2817 3623 4220 5007 5275 1239 Size: 393 1185 806 597 787 268 1524 Fragments arranged by size: 1524 1185 806 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2610 2610 Size: 5560 EcoO109I rG'GnC_Cy Cuts at: 2221 2221 Size: 5560 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2416 4707 1678 Size: 738 2291 2531 Fragments arranged by size: 2531 2291 738 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373 Size: 32 135 32 2629 32 42 32 2626 Fragments arranged by size: 2629 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2402 4674 4858 1305 Size: 1097 2272 184 2007 Fragments arranged by size: 2272 2007 1097 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3738 Fragments arranged by size: 3738 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3518 3877 4036 518 Size: 648 843 1509 359 159 2042 Fragments arranged by size: 2042 1509 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2976 3653 4801 5046 1606 Size: 423 947 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4069 Fragments arranged by size: 4069 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PfoI T'CCnGG_A Cuts at: 2178 2178 Size: 5560 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2221 2221 Size: 5560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PstI C_TGCA'G Cuts at: 830 2434 2912 830 Size: 1604 478 3478 Fragments arranged by size: 3478 1604 478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SacII CC_GC'GG Cuts at: 2030 2030 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 2639 3808 5520 2639 Size: 1169 1712 2679 Fragments arranged by size: 2679 1712 1169 SfcI C'TryA_G Cuts at: 826 1045 2041 2250 2430 2607 2908 4043 4724 Size: 219 996 209 180 177 301 1135 681 Cuts at: 4724 4915 826 Size: 191 1471 Fragments arranged by size: 1471 1135 996 681 301 219 209 191 180 177 SfiI GGCCn_nnn'nGGCC Cuts at: 2562 2562 Size: 5560 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2497 3664 4565 4827 820 Size: 197 926 165 389 1167 901 262 1553 Fragments arranged by size: 1553 1167 926 901 389 262 197 165 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 2465 152 Size: 2313 3247 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 2556 2616 1517 Size: 1039 60 4461 Fragments arranged by size: 4461 1039 60 StyI C'CwwG_G Cuts at: 513 1065 2004 2999 513 Size: 552 939 995 3074 Fragments arranged by size: 3074 995 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2260 3587 3604 3757 3942 4387 325 Size: 1255 680 1327 17 153 185 445 1498 Fragments arranged by size: 1498 1327 1255 680 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 3806 5518 Size: 274 80 33 51 1443 660 1169 1712 Cuts at: 5518 96 Size: 138 Fragments arranged by size: 1712 1443 1169 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 2830 3689 2830 Size: 859 4701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI XcmI