(Circular) (Six-base) MAPSORT of: pFN21AE0338.seq Check: 2066 from: 1 to: 6220 pFN21AE0338 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2586 2945 4283 5667 5935 1632 Size: 714 240 359 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 714 359 268 240 AflII C'TTAA_G Cuts at: 820 1017 2542 820 Size: 197 1525 4498 Fragments arranged by size: 4498 1525 197 AflIII A'CryG_T Cuts at: 1203 3040 5119 6096 1203 Size: 1837 2079 977 1327 Fragments arranged by size: 2079 1837 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 2942 3963 5535 2942 Size: 1021 1572 3627 Fragments arranged by size: 3627 1572 1021 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3309 3545 1870 Size: 73 1366 236 4545 Fragments arranged by size: 4545 1366 236 73 BamHI G'GATC_C Cuts at: 3131 3550 3917 3131 Size: 419 367 5434 Fragments arranged by size: 5434 419 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3212 3541 4996 618 Size: 325 217 627 1425 329 1455 1842 Fragments arranged by size: 1842 1455 1425 627 329 325 217 BanII G_rGCy'C Cuts at: 1391 3539 1391 Size: 2148 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2574 3001 928 Size: 971 675 427 4147 Fragments arranged by size: 4147 971 675 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2347 4112 5322 2347 Size: 1765 1210 3245 Fragments arranged by size: 3245 1765 1210 BclI T'GATC_A Cuts at: 1548 3510 1548 Size: 1962 4258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2088 4281 5437 1790 Size: 298 2193 1156 2573 Fragments arranged by size: 2573 2193 1156 298 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 2150 4880 1239 Size: 844 67 2730 2579 Fragments arranged by size: 2730 2579 844 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3260 3490 4883 882 Size: 617 1761 230 1393 2219 Fragments arranged by size: 2219 1761 1393 617 230 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 2600 3141 1186 Size: 1339 75 541 4265 Fragments arranged by size: 4265 1339 541 75 BseYI C'CCAG_C Cuts at: 2942 5423 5919 2942 Size: 2481 496 3243 Fragments arranged by size: 3243 2481 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 2893 3204 4281 4366 5437 2580 Size: 313 311 1077 85 1071 3363 Fragments arranged by size: 3363 1077 1071 313 311 85 BsmI GAATG_Cn' Cuts at: 2164 2500 3731 3824 2164 Size: 336 1231 93 4560 Fragments arranged by size: 4560 1231 336 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2580 2893 3204 3539 4281 4366 Size: 399 298 492 313 311 335 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 335 313 311 298 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 3464 3575 844 Size: 1242 1378 111 3489 Fragments arranged by size: 3489 1378 1242 111 BsrBI CCG'CTC Cuts at: 1394 1823 2967 3293 4107 1394 Size: 429 1144 326 814 3507 Fragments arranged by size: 3507 1144 814 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 2221 3066 3165 4715 4889 65 Size: 1183 973 845 99 1550 174 1396 Fragments arranged by size: 1550 1396 1183 973 845 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 2506 3188 3467 2506 Size: 682 279 5259 Fragments arranged by size: 5259 682 279 BstYI r'GATC_y Cuts at: 1972 3131 3550 3917 4302 4319 5771 5857 5869 Size: 1159 419 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1159 419 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3216 513 Size: 552 2151 3517 Fragments arranged by size: 3517 2151 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2578 3736 4530 4550 2578 Size: 1158 794 20 4248 Fragments arranged by size: 4248 1158 794 20 ClaI AT'CG_AT Cuts at: 1554 2245 1554 Size: 691 5529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3667 4556 6185 Size: 54 1425 50 275 18 1837 889 1629 Cuts at: 6185 8 Size: 43 Fragments arranged by size: 1837 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2843 2974 3405 4150 1353 Size: 1490 131 431 745 3423 Fragments arranged by size: 3423 1490 745 431 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2150 2346 2586 2945 4283 4880 Size: 393 451 67 196 240 359 1338 597 Cuts at: 4880 5667 5935 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1338 787 597 451 393 359 268 240 196 67 EcoO109I rG'GnC_Cy Cuts at: 2664 2664 Size: 6220 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373 Size: 32 135 32 3289 32 42 32 2626 Fragments arranged by size: 3289 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3564 3810 669 Size: 988 281 1024 602 246 3079 Fragments arranged by size: 3079 1024 988 602 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3501 3819 3501 Size: 318 5902 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 4178 4537 4696 518 Size: 648 1323 1689 359 159 2042 Fragments arranged by size: 2042 1689 1323 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2939 3636 4313 5461 5706 1606 Size: 1333 697 677 1148 245 2120 Fragments arranged by size: 2120 1333 1148 697 677 245 NcoI C'CATG_G Cuts at: 513 1065 3216 513 Size: 552 2151 3517 Fragments arranged by size: 3517 2151 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2095 3044 3089 3578 5123 1132 Size: 97 866 949 45 489 1545 2229 Fragments arranged by size: 2229 1545 949 866 489 97 45 PasI CC'CwG_GG Cuts at: 1348 2668 1348 Size: 1320 4900 PciI A'CATG_T Cuts at: 3040 5119 3040 Size: 2079 4141 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 1108 Size: 1596 4624 PfoI T'CCnGG_A Cuts at: 3339 3339 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2018 2050 4289 4321 5802 5834 2018 Size: 32 2239 32 1481 32 2404 Fragments arranged by size: 2404 2239 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2664 2664 Size: 6220 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 2034 2607 3572 830 Size: 1204 573 965 3478 Fragments arranged by size: 3478 1204 965 573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2939 2939 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SanDI GG'GwC_CC Cuts at: 2664 2664 Size: 6220 SapI GCTCTTCn'nnn_ Cuts at: 2974 2974 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2030 2603 3226 3568 4703 5384 5575 Size: 219 985 573 623 342 1135 681 191 Cuts at: 5575 826 Size: 1471 Fragments arranged by size: 1471 1135 985 681 623 573 342 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2542 4324 5225 5487 820 Size: 197 926 599 1782 901 262 1553 Fragments arranged by size: 1782 1553 926 901 599 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 2516 2649 1517 Size: 999 133 5088 Fragments arranged by size: 5088 999 133 StyI C'CwwG_G Cuts at: 513 1065 2517 3003 3216 3659 513 Size: 552 1452 486 213 443 3074 Fragments arranged by size: 3074 1452 552 486 443 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3409 4247 4264 4417 4602 5047 325 Size: 1255 1829 838 17 153 185 445 1498 Fragments arranged by size: 1829 1498 1255 838 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3118 4466 6178 Size: 274 80 33 51 1443 1141 1348 1712 Cuts at: 6178 96 Size: 138 Fragments arranged by size: 1712 1443 1348 1141 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3183 3215 6180 6212 3183 Size: 32 2965 32 3191 Fragments arranged by size: 3191 2965 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2276 2459 2821 3403 2276 Size: 183 362 582 5093 Fragments arranged by size: 5093 582 362 183 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2078 2899 4349 2078 Size: 821 1450 3949 Fragments arranged by size: 3949 1450 821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI