Restriction map for
pFN21AE0521
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 394 GACGTC GACGT^C
AcsI 82 GAATTC [GA]^AATT[CT]
AhdI 301 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 544 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 82 GAATTC [GA]^AATT[CT]
AspEI 301 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 254 CCCGAG C^[CT]CG[GA]G
BcnI 618 CCCGG CC^[GC]GG
BsgI 649 GTGCAG GTGCAG(16/14)
BsmBI 392 GAGACG CGTCTC(1/5)
BsoBI 254 CCCGAG C^[CT]CG[GA]G
Bsp1407I 344 TGTACA T^GTACA
BspMI 227 ACCTGC ACCTGC(4/8)
BsrGI 344 TGTACA T^GTACA
BstAPI 651 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 134 AGATCC [GA]^GATC[CT]
DraII 779 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 301 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 315 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 779 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 82 GAATTC G^AATTC
EcoT14I 554 CCAAGG C^C[AT][AT]GG
HaeII 546 GGCGCT [GA]GCGC^[CT]
HapII 619 CCGG C^CGG
HgaI 444 GACGC GACGC(5/10)
HinfI 483 GACTC G^A[ACGT]TC
HpaII 619 CCGG C^CGG
MflI 134 AGATCC [GA]^GATC[CT]
MluI 47 ACGCGT A^CGCGT
MseI 17 TTAA T^TAA
MslI 291 CACTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 619 CCGG C^CGG
NciI 618 CCCGG CC^[GC]GG
PleI 483 GACTC GAGTC(4/5)
PpuMI 779 AGGACCT [GA]G^G[AT]CC[CT]
SapI 516 GCTCTTC GCTCTTC(1/4)
SspBI 344 TGTACA T^GTACA
StyI 554 CCAAGG C^C[AT][AT]GG
TaqI 626 TCGA T^CGA
Tru9I 17 TTAA T^TAA
Tsp509I 83 AATT ^AATT
TthHB8I 626 TCGA T^CGA
XcmI 339 CCATCTGTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 134 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 394 GACGTC G[GA]^CG[CT]C
444 GACGCC
AflIII 47 ACGCGT A^C[GA][CT]GT
196 ACACGT
AvaII 630 GGACC G^G[AT]CC
780 GGACC
BanI 451 GGCACC G^G[CT][GA]CC
703 GGCACC
BbrPI 197 CACGTG CAC^GTG
745 CACGTG
BlpI 462 GCTGAGC GC^T[ACGT]AGC
660 GCTGAGC
Bpu1102I 462 GCTGAGC GC^T[ACGT]AGC
660 GCTGAGC
BsaHI 394 GACGTC G[GA]^CG[CT]C
444 GACGCC
BsaI 796 GAGACC GGTCTC(1/5)
324 GGTCTC
BsrI 245 ACTGG ACTGG(1/-1)
791 ACTGG
BstXI 290 CCACTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
572 CCAGCCTCCTGG
CelII 462 GCTGAGC GC^T[ACGT]AGC
660 GCTGAGC
EaeI 620 CGGCCA [CT]^GGCC[GA]
741 CGGCCA
EarI 517 CTCTTC CTCTTC(1/4)
598 GAAGAG
Hin1I 394 GACGTC G[GA]^CG[CT]C
444 GACGCC
Ksp632I 517 CTCTTC CTCTTC(1/4)
598 GAAGAG
MaeIII 274 GTCAC ^GT[ACGT]AC
748 GTGAC
MspA1I 238 CAGCTG C[AC]G^C[GT]G
607 CAGCTG
PmaCI 197 CACGTG CAC^GTG
745 CACGTG
PmlI 197 CACGTG CAC^GTG
745 CACGTG
PstI 229 CTGCAG CTGCA^G
820 CTGCAG
PvuII 238 CAGCTG CAG^CTG
607 CAGCTG
Tsp45I 274 GTCAC ^GT[GC]AC
748 GTGAC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BbvI BmyI BpmI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MaeII MboI
MboII MnlI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI