Restriction map for
pFN21AE0521
(Linear) (Six-base) MAPSORT of: KIEE0521.seq Check: 472 from: 1 to: 828
KIEE0521.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 0 398 828
Size: 398 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 828
Size: 337 491
AflIII A'CryG_T
Cuts at: 0 47 196 828
Size: 47 149 632
Fragments arranged by size:
632 149 47
AhdI GACnn_n'nnGTC
Cuts at: 0 306 828
Size: 306 522
AleI CACnn'nnGTG
Cuts at: 0 295 828
Size: 295 533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 828
Size: 490 338
AlwNI CAG_nnn'CTG
Cuts at: 0 549 828
Size: 549 279
ApoI r'AATT_y
Cuts at: 0 82 828
Size: 82 746
AvaI C'yCGr_G
Cuts at: 0 254 828
Size: 254 574
BanI G'GyrC_C
Cuts at: 0 451 703 828
Size: 451 252 125
Fragments arranged by size:
451 252 125
BlpI GC'TnA_GC
Cuts at: 0 463 661 828
Size: 463 198 167
Fragments arranged by size:
463 198 167
BmgBI CAC'GTC
Cuts at: 0 297 828
Size: 297 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 801 828
Size: 801 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 510 544 828
Size: 499 11 34 284
Fragments arranged by size:
499 284 34 11
Bpu10I CC'TnA_GC
Cuts at: 0 376 828
Size: 376 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 330 790 828
Size: 330 460 38
Fragments arranged by size:
460 330 38
BsaAI yAC'GTr
Cuts at: 0 199 648 747 828
Size: 199 449 99 81
Fragments arranged by size:
449 199 99 81
BsaHI Gr'CG_yC
Cuts at: 0 395 445 828
Size: 395 50 383
Fragments arranged by size:
395 383 50
BseYI C'CCAG_C
Cuts at: 0 183 828
Size: 183 645
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 828
Size: 670 158
BsiHKAI G_wGCw'C
Cuts at: 0 382 566 590 828
Size: 382 184 24 238
Fragments arranged by size:
382 238 184 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 386 828
Size: 386 442
Bsp1286I G_dGCh'C
Cuts at: 0 382 566 590 828
Size: 382 184 24 238
Fragments arranged by size:
382 238 184 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 828
Size: 236 592
BsrGI T'GTAC_A
Cuts at: 0 344 828
Size: 344 484
BstAPI GCAn_nnn'nTGC
Cuts at: 0 657 828
Size: 657 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 579 828
Size: 297 282 249
Fragments arranged by size:
297 282 249
BstYI r'GATC_y
Cuts at: 0 134 828
Size: 134 694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 552 828
Size: 552 276
BtsI GCAGTG_nn'
Cuts at: 0 817 828
Size: 817 11
EaeI y'GGCC_r
Cuts at: 0 620 741 828
Size: 620 121 87
Fragments arranged by size:
620 121 87
EarI CTCTTCn'nnn_
Cuts at: 0 523 593 828
Size: 523 70 235
Fragments arranged by size:
523 235 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 499 510 544 828
Size: 337 162 11 34 284
Fragments arranged by size:
337 284 162 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 828
Size: 319 509
EcoO109I rG'GnC_Cy
Cuts at: 0 780 828
Size: 780 48
EcoRI G'AATT_C
Cuts at: 0 82 828
Size: 82 746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 519 534 828
Size: 519 15 294
Fragments arranged by size:
519 294 15
HaeII r_GCGC'y
Cuts at: 0 550 828
Size: 550 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 828
Size: 292 32 504
Fragments arranged by size:
504 292 32
MluI A'CGCG_T
Cuts at: 0 47 828
Size: 47 781
MslI CAynn'nnrTG
Cuts at: 0 295 828
Size: 295 533
MspA1I CmG'CkG
Cuts at: 0 240 609 828
Size: 240 369 219
Fragments arranged by size:
369 240 219
PfoI T'CCnGG_A
Cuts at: 0 521 578 828
Size: 521 57 250
Fragments arranged by size:
521 250 57
PmlI CAC'GTG
Cuts at: 0 199 747 828
Size: 199 548 81
Fragments arranged by size:
548 199 81
PpuMI rG'GwC_Cy
Cuts at: 0 780 828
Size: 780 48
PstI C_TGCA'G
Cuts at: 0 233 824 828
Size: 233 591 4
Fragments arranged by size:
591 233 4
PvuII CAG'CTG
Cuts at: 0 240 609 828
Size: 240 369 219
Fragments arranged by size:
369 240 219
SapI GCTCTTCn'nnn_
Cuts at: 0 523 828
Size: 523 305
SfcI C'TryA_G
Cuts at: 0 87 229 820 828
Size: 87 142 591 8
Fragments arranged by size:
591 142 87 8
StyI C'CwwG_G
Cuts at: 0 554 828
Size: 554 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 828
Size: 292 536
TatI w'GTAC_w
Cuts at: 0 344 828
Size: 344 484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 828
Size: 346 482
ZraI GAC'GTC
Cuts at: 0 396 828
Size: 396 432
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AlfI AlwNI ApoI
AvaI BanI BlpI BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrGI BstAPI BstXI BstYI BtgZI BtsI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MluI
MslI MspA1I PfoI PmlI PpuMI PstI PvuII SapI
SfcI StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmtI BplI BpuEI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI