(Circular) (Six-base) MAPSORT of: pFN21AE0414.seq Check: 2936 from: 1 to: 5263 pFN21AE0414 5263 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3073 278 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 1937 Size: 669 4594 Acc65I G'GTAC_C Cuts at: 2584 2584 Size: 5263 AclI AA'CG_TT Cuts at: 1563 3390 3763 1563 Size: 1827 373 3063 Fragments arranged by size: 3063 1827 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3326 4710 4978 1632 Size: 785 909 1384 268 1917 Fragments arranged by size: 1917 1384 909 785 268 AfeI AGC'GCT Cuts at: 2300 2300 Size: 5263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5066 AflIII A'CryG_T Cuts at: 1203 2172 4162 5139 1203 Size: 969 1990 977 1327 Fragments arranged by size: 1990 1327 977 969 AgeI A'CCGG_T Cuts at: 4094 4094 Size: 5263 AhdI GACnn_n'nnGTC Cuts at: 3992 3992 Size: 5263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5263 AlwNI CAG_nnn'CTG Cuts at: 3006 4578 3006 Size: 1572 3691 ApaLI G'TGCA_C Cuts at: 2351 3320 4476 2351 Size: 969 1156 3138 Fragments arranged by size: 3138 1156 969 ApoI r'AATT_y Cuts at: 1493 2572 2798 2950 5059 1493 Size: 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 3817 160 Size: 872 2785 1606 Fragments arranged by size: 2785 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 AvaI C'yCGr_G Cuts at: 1870 1943 2179 2222 2588 1870 Size: 73 236 43 366 4545 Fragments arranged by size: 4545 366 236 73 43 BamHI G'GATC_C Cuts at: 2593 2960 2593 Size: 367 4896 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2473 2584 4039 618 Size: 325 217 627 318 368 111 1455 1842 Fragments arranged by size: 1842 1455 627 368 325 318 217 111 BanII G_rGCy'C Cuts at: 1391 2529 2582 1391 Size: 1138 53 4072 Fragments arranged by size: 4072 1138 53 BbeI G_GCGC'C Cuts at: 2109 2109 Size: 5263 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3454 3488 1903 Size: 34 1517 34 3678 Fragments arranged by size: 3678 1517 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3155 4365 3155 Size: 1210 4053 BclI T'GATC_A Cuts at: 1548 1548 Size: 5263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3874 136 Size: 107 122 71 631 605 2202 1525 Fragments arranged by size: 2202 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5258 5258 Size: 5263 BlpI GC'TnA_GC Cuts at: 2680 2680 Size: 5263 Bme1580I G_kGCm'C Cuts at: 1790 2355 2478 3324 4480 1790 Size: 565 123 846 1156 2573 Fragments arranged by size: 2573 1156 846 565 123 BmgBI CAC'GTC Cuts at: 1318 2350 2470 1318 Size: 1032 120 4111 Fragments arranged by size: 4111 1032 120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2994 3952 442 Size: 416 53 83 399 24 1577 958 1753 Fragments arranged by size: 1753 1577 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2257 2257 Size: 5263 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 3923 1239 Size: 1106 1578 2579 Fragments arranged by size: 2579 1578 1106 Bpu10I CC'TnA_GC Cuts at: 2312 2312 Size: 5263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3388 4253 4551 3388 Size: 865 298 4100 Fragments arranged by size: 4100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3926 882 Size: 617 2427 2219 Fragments arranged by size: 2427 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2173 3054 493 Size: 711 969 881 2702 Fragments arranged by size: 2702 969 881 711 BsaBI GATnn'nnATC Cuts at: 5257 5257 Size: 5263 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 3070 3452 4087 Size: 53 83 186 936 573 964 382 635 Cuts at: 4087 275 Size: 1451 Fragments arranged by size: 1451 964 936 635 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3696 4094 4368 4515 4926 3696 Size: 398 274 147 411 4033 Fragments arranged by size: 4033 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5038 Fragments arranged by size: 5038 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 1186 Size: 897 4366 BseYI C'CCAG_C Cuts at: 2332 4466 4962 2332 Size: 2134 496 2633 Fragments arranged by size: 2633 2134 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 1163 Size: 573 678 4012 Fragments arranged by size: 4012 678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2445 2713 3474 4502 1540 Size: 275 186 444 268 761 1028 2301 Fragments arranged by size: 2301 1028 761 444 275 268 186 BsiHKAI G_wGCw'C Cuts at: 2355 2529 3324 3409 4480 2355 Size: 174 795 85 1071 3138 Fragments arranged by size: 3138 1071 795 174 85 BsmI GAATG_Cn' Cuts at: 2774 2867 2774 Size: 93 5170 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2355 2478 2529 2582 3324 3409 4480 Size: 399 565 123 51 53 742 85 1071 Cuts at: 4480 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 565 399 123 85 53 51 BspEI T'CCGG_A Cuts at: 4926 4926 Size: 5263 BspHI T'CATG_A Cuts at: 3152 4882 3152 Size: 1730 3533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2421 2618 844 Size: 1577 197 3489 Fragments arranged by size: 3489 1577 197 BsrBI CCG'CTC Cuts at: 1394 1823 3150 1394 Size: 429 1327 3507 Fragments arranged by size: 3507 1327 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3758 3932 65 Size: 1183 2510 174 1396 Fragments arranged by size: 2510 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3907 4094 1700 Size: 110 2097 187 2869 Fragments arranged by size: 2869 2097 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5263 BssHII G'CGCG_C Cuts at: 1925 2120 2316 2318 2416 1925 Size: 195 196 2 98 4772 Fragments arranged by size: 4772 196 195 98 2 BssSI C'ACGA_G Cuts at: 3323 4335 3323 Size: 1012 4251 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5263 BstBI TT'CG_AA Cuts at: 4067 4067 Size: 5263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5263 BstXI CCAn_nnnn'nTGG Cuts at: 2254 2488 2254 Size: 234 5029 BstYI r'GATC_y Cuts at: 1972 2088 2593 2960 3345 3362 4814 4900 4912 Size: 116 505 367 385 17 1452 86 12 Cuts at: 4912 5258 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 505 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2265 513 Size: 552 939 261 3511 Fragments arranged by size: 3511 939 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4524 Fragments arranged by size: 4524 683 56 BtsI GCAGTG_nn' Cuts at: 2779 3573 3593 2779 Size: 794 20 4449 Fragments arranged by size: 4449 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5228 DraI TTT'AAA Cuts at: 2567 2567 Size: 5263 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4270 809 Size: 507 2954 1802 Fragments arranged by size: 2954 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2442 2710 Size: 54 1425 50 275 18 381 231 268 Cuts at: 2710 3599 5228 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 381 275 268 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2442 2710 1537 Size: 275 630 268 4090 Fragments arranged by size: 4090 630 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 2558 3193 1353 Size: 1205 635 3423 Fragments arranged by size: 3423 1205 635 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3850 4224 4370 387 Size: 1064 138 2261 374 146 1280 Fragments arranged by size: 2261 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2527 2527 Size: 5263 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2417 3326 3923 4710 4978 1239 Size: 393 713 72 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 713 597 393 268 72 EcoRI G'AATT_C Cuts at: 2572 2950 2572 Size: 378 4885 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2569 2569 Size: 5263 FspI TGC'GCA Cuts at: 2453 3769 2453 Size: 1316 3947 HaeII r_GCGC'y Cuts at: 1678 2109 2302 4410 1678 Size: 431 193 2108 2531 Fragments arranged by size: 2531 2108 431 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3904 3936 3978 4010 1373 Size: 32 135 32 2332 32 42 32 2626 Fragments arranged by size: 2626 2332 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2607 2853 669 Size: 988 281 669 246 3079 Fragments arranged by size: 3079 988 669 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5263 HpaI GTT'AAC Cuts at: 2853 2853 Size: 5263 KasI G'GCGC_C Cuts at: 2105 2105 Size: 5263 KpnI G_GTAC'C Cuts at: 2588 2588 Size: 5263 MfeI C'AATT_G Cuts at: 2862 2862 Size: 5263 MluI A'CGCG_T Cuts at: 5139 5139 Size: 5263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4377 4561 1305 Size: 3072 184 2007 Fragments arranged by size: 3072 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 10 Size: 54 1425 343 381 3060 Fragments arranged by size: 3060 1425 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3221 3580 3739 518 Size: 648 1302 753 359 159 2042 Fragments arranged by size: 2042 1302 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2404 2679 3356 4504 4749 1606 Size: 543 255 275 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 275 255 245 NarI GG'CG_CC Cuts at: 2106 2106 Size: 5263 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3772 Fragments arranged by size: 3772 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5263 NotI GC'GGCC_GC Cuts at: 2710 2710 Size: 5263 NspI r_CATG'y Cuts at: 1132 1229 2621 4166 1132 Size: 97 1392 1545 2229 Fragments arranged by size: 2229 1545 1392 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5263 PciI A'CATG_T Cuts at: 4162 4162 Size: 5263 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5263 PmeI GTTT'AAAC Cuts at: 2567 2567 Size: 5263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3332 3364 4845 4877 3332 Size: 32 1481 32 3718 Fragments arranged by size: 3718 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5263 PsiI TTA'TAA Cuts at: 2833 2833 Size: 5263 PstI C_TGCA'G Cuts at: 830 2615 830 Size: 1785 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5263 PvuII CAG'CTG Cuts at: 2149 2149 Size: 5263 SacI G_AGCT'C Cuts at: 2529 2529 Size: 5263 SalI G'TCGA_C Cuts at: 1936 2605 1936 Size: 669 4594 SbfI CC_TGCA'GG Cuts at: 2615 2615 Size: 5263 ScaI AGT'ACT Cuts at: 3511 5223 3511 Size: 1712 3551 SfcI C'TryA_G Cuts at: 826 1045 2338 2611 3746 4427 4618 826 Size: 219 1293 273 1135 681 191 1471 Fragments arranged by size: 1471 1293 1135 681 273 219 191 SfoI GGC'GCC Cuts at: 2107 2107 Size: 5263 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5263 SmaI CCC'GGG Cuts at: 1872 2181 2590 1872 Size: 309 409 4545 Fragments arranged by size: 4545 409 309 SmlI C'TyrA_G Cuts at: 820 1017 1943 3367 4268 4530 820 Size: 197 926 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3054 493 Size: 2561 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5263 SphI G_CATG'C Cuts at: 1132 2621 1132 Size: 1489 3774 SspI AAT'ATT Cuts at: 5 52 3187 5 Size: 47 3135 2081 Fragments arranged by size: 3135 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5263 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2702 513 Size: 552 939 528 170 3074 Fragments arranged by size: 3074 939 552 528 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3290 3307 3460 3645 4090 325 Size: 1255 1710 17 153 185 445 1498 Fragments arranged by size: 1710 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3509 5221 96 Size: 274 80 33 51 1443 1532 1712 138 Fragments arranged by size: 1712 1532 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5223 5255 5223 Size: 32 5231 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5263 XbaI T'CTAG_A Cuts at: 2599 2599 Size: 5263 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2251 Size: 5263 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5263 XmaI C'CCGG_G Cuts at: 1870 2179 2588 1870 Size: 309 409 4545 Fragments arranged by size: 4545 409 309 XmnI GAAnn'nnTTC Cuts at: 3392 3392 Size: 5263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3071 276 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI