(Circular) (Six-base) MAPSORT of: pFN21AB6477.seq Check: 2149 from: 1 to: 5683 pFN21AB6477 5683 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 3026 1937 Size: 849 240 4594 Fragments arranged by size: 4594 849 240 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 3746 5130 5398 1632 Size: 767 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 2123 4412 2123 Size: 2289 3394 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2080 2377 2563 3426 4998 2080 Size: 297 186 863 1572 2765 Fragments arranged by size: 2765 1572 863 297 186 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2231 2243 2460 2992 3218 3370 5479 1493 Size: 738 12 217 532 226 152 2109 1697 Fragments arranged by size: 2109 1697 738 532 226 217 152 12 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 3008 1870 Size: 73 1065 4545 Fragments arranged by size: 4545 1065 73 BamHI G'GATC_C Cuts at: 2509 3013 3380 2509 Size: 504 367 4812 Fragments arranged by size: 4812 504 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 2730 3004 4459 618 Size: 325 217 627 349 594 274 1455 1842 Fragments arranged by size: 1842 1455 627 594 349 325 274 217 BanII G_rGCy'C Cuts at: 1391 2148 2408 3002 1391 Size: 757 260 594 4072 Fragments arranged by size: 4072 757 594 260 BbeI G_GCGC'C Cuts at: 2734 2734 Size: 5683 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 4294 136 Size: 107 122 71 631 605 616 2006 1525 Fragments arranged by size: 2006 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 2051 2941 3100 2051 Size: 890 159 4634 Fragments arranged by size: 4634 890 159 Bme1580I G_kGCm'C Cuts at: 1790 3744 4900 1790 Size: 1954 1156 2573 Fragments arranged by size: 2573 1954 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2076 2076 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2768 4343 1239 Size: 812 717 1575 2579 Fragments arranged by size: 2579 1575 812 717 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2731 3490 3872 4507 Size: 53 83 186 936 1198 759 382 635 Cuts at: 4507 275 Size: 1451 Fragments arranged by size: 1451 1198 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2486 2493 1186 Size: 1297 3 7 4376 Fragments arranged by size: 4376 1297 7 3 BseYI C'CCAG_C Cuts at: 2960 4886 5382 2960 Size: 1926 496 3261 Fragments arranged by size: 3261 1926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 1163 Size: 573 357 4753 Fragments arranged by size: 4753 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 3744 3829 4900 2148 Size: 1596 85 1071 2931 Fragments arranged by size: 2931 1596 1071 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 BsmBI CGTCTCn'nnnn_ Cuts at: 2131 2131 Size: 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2408 3002 3744 3829 4900 1391 Size: 399 358 260 594 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 399 358 260 85 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 844 Size: 2194 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 2856 3743 4755 2856 Size: 887 1012 3784 Fragments arranged by size: 3784 1012 887 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 794 Size: 1277 4406 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2509 2560 3013 3380 3765 3782 5234 5320 Size: 537 51 453 367 385 17 1452 86 Cuts at: 5320 5332 5678 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 537 453 385 367 346 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2787 2787 Size: 5683 Bsu36I CC'TnA_GG Cuts at: 2505 2505 Size: 5683 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2915 3199 3993 4013 2915 Size: 284 794 20 4585 Fragments arranged by size: 4585 794 284 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648 Size: 54 1425 50 275 18 1300 889 1629 Cuts at: 5648 8 Size: 43 Fragments arranged by size: 1629 1425 1300 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 3613 1353 Size: 1068 1192 3423 Fragments arranged by size: 3423 1192 1068 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2399 2768 3746 4343 5130 5398 Size: 393 419 348 369 978 597 787 268 Cuts at: 5398 1239 Size: 1524 Fragments arranged by size: 1524 978 787 597 419 393 369 348 268 EcoNI CCTnn'n_nnAGG Cuts at: 2517 2517 Size: 5683 EcoO109I rG'GnC_Cy Cuts at: 2117 2225 2522 2965 2117 Size: 108 297 443 4835 Fragments arranged by size: 4835 443 297 108 EcoRI G'AATT_C Cuts at: 2231 2992 3370 2231 Size: 761 378 4544 Fragments arranged by size: 4544 761 378 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2030 2734 4830 1678 Size: 352 704 2096 2531 Fragments arranged by size: 2531 2096 704 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 2420 2452 2795 Size: 32 135 32 537 32 279 32 343 Cuts at: 2795 2827 4324 4356 4398 4430 1373 Size: 32 1497 32 42 32 2626 Fragments arranged by size: 2626 1497 537 343 279 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 2171 2550 2849 748 Size: 1423 379 299 3582 Fragments arranged by size: 3582 1423 379 299 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KasI G'GCGC_C Cuts at: 2730 2730 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2620 2793 4797 4981 1305 Size: 1315 173 2004 184 2007 Fragments arranged by size: 2007 2004 1315 184 173 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2377 2672 2764 3099 3776 4924 5169 1606 Size: 771 295 92 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 677 335 295 245 92 NarI GG'CG_CC Cuts at: 2731 2731 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5131 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2706 3041 4586 1132 Size: 97 1477 335 1545 2229 Fragments arranged by size: 2229 1545 1477 335 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2117 2225 2522 2965 2117 Size: 108 297 443 4835 Fragments arranged by size: 4835 443 297 108 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2521 3035 830 Size: 1691 514 3478 Fragments arranged by size: 3478 1691 514 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2377 2672 2764 2377 Size: 295 92 5296 Fragments arranged by size: 5296 295 92 SacI G_AGCT'C Cuts at: 2148 2148 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SanDI GG'GwC_CC Cuts at: 2117 2117 Size: 5683 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2517 3031 4166 4847 5038 826 Size: 219 1472 514 1135 681 191 1471 Fragments arranged by size: 1472 1471 1135 681 514 219 191 SfoI GGC'GCC Cuts at: 2732 2732 Size: 5683 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 3122 513 Size: 552 2057 3074 Fragments arranged by size: 3074 2057 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 3727 3880 4065 4510 325 Size: 1255 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 2080 1316 Size: 764 4919 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI