(Circular) (Six-base) MAPSORT of: pFN21AE0330.seq Check: 9425 from: 1 to: 6529 pFN21AE0330 6529 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4339 278 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3872 1937 Size: 1935 4594 Acc65I G'GTAC_C Cuts at: 3850 3850 Size: 6529 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2814 3098 3260 3340 4592 5976 6244 1632 Size: 1182 284 162 80 1252 1384 268 1917 Fragments arranged by size: 1917 1384 1252 1182 284 268 162 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6332 AflIII A'CryG_T Cuts at: 1203 5428 6405 1203 Size: 4225 977 1327 Fragments arranged by size: 4225 1327 977 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 3297 5258 3297 Size: 1961 4568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6529 AlwNI CAG_nnn'CTG Cuts at: 2263 2295 4272 5844 2263 Size: 32 1977 1572 2948 Fragments arranged by size: 2948 1977 1572 32 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 6529 ApaLI G'TGCA_C Cuts at: 4586 5742 4586 Size: 1156 5373 ApoI r'AATT_y Cuts at: 1493 2579 3305 3838 4064 4216 6325 1493 Size: 1086 726 533 226 152 2109 1697 Fragments arranged by size: 2109 1697 1086 726 533 226 152 AseI AT'TA_AT Cuts at: 160 1032 5083 160 Size: 872 4051 1606 Fragments arranged by size: 4051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2206 3854 1870 Size: 73 106 157 1648 4545 Fragments arranged by size: 4545 1648 157 106 73 AvrII C'CTAG_G Cuts at: 2584 2584 Size: 6529 BamHI G'GATC_C Cuts at: 2030 3859 4226 2030 Size: 1829 367 4333 Fragments arranged by size: 4333 1829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 3523 3684 3850 5305 Size: 325 217 627 364 1372 161 166 1455 Cuts at: 5305 618 Size: 1842 Fragments arranged by size: 1842 1455 1372 627 364 325 217 166 161 BanII G_rGCy'C Cuts at: 1391 2116 2229 2454 2792 3318 3402 3848 1391 Size: 725 113 225 338 526 84 446 4072 Fragments arranged by size: 4072 725 526 446 338 225 113 84 BbeI G_GCGC'C Cuts at: 2155 2155 Size: 6529 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 928 Size: 971 690 4868 Fragments arranged by size: 4868 971 690 BbvCI CC'TCA_GC Cuts at: 3189 3693 3189 Size: 504 6025 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4720 4754 1903 Size: 34 50 34 2699 34 3678 Fragments arranged by size: 3678 2699 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 1548 Size: 6529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 3065 5140 Size: 107 122 71 631 605 371 1022 2075 Cuts at: 5140 136 Size: 1525 Fragments arranged by size: 2075 1525 1022 631 605 371 122 107 71 BglII A'GATC_T Cuts at: 2590 6524 2590 Size: 3934 2595 BlpI GC'TnA_GC Cuts at: 2363 3647 3946 2363 Size: 1284 299 4946 Fragments arranged by size: 4946 1284 299 Bme1580I G_kGCm'C Cuts at: 1790 2116 3553 4590 5746 1790 Size: 326 1437 1037 1156 2573 Fragments arranged by size: 2573 1437 1156 1037 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3547 4260 5218 Size: 416 53 83 399 24 2130 713 958 Cuts at: 5218 442 Size: 1753 Fragments arranged by size: 2130 1753 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2933 3332 5189 1239 Size: 1694 399 1857 2579 Fragments arranged by size: 2579 1857 1694 399 Bpu10I CC'TnA_GC Cuts at: 3189 3693 3189 Size: 504 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2377 3054 4654 5519 5817 2377 Size: 677 1600 865 298 3089 Fragments arranged by size: 3089 1600 865 677 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5192 882 Size: 617 3693 2219 Fragments arranged by size: 3693 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4320 493 Size: 711 3116 2702 Fragments arranged by size: 3116 2702 711 BsaBI GATnn'nnATC Cuts at: 6523 6523 Size: 6529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2152 2241 4336 4718 Size: 53 83 186 936 619 89 2095 382 Cuts at: 4718 5353 275 Size: 635 1451 Fragments arranged by size: 2095 1451 936 635 619 382 186 89 83 53 BsaWI w'CCGG_w Cuts at: 4962 5360 5634 5781 6192 4962 Size: 398 274 147 411 5299 Fragments arranged by size: 5299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6304 Fragments arranged by size: 6304 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 3206 3533 1186 Size: 943 1077 327 4182 Fragments arranged by size: 4182 1077 943 327 BseYI C'CCAG_C Cuts at: 3725 5732 6228 3725 Size: 2007 496 4026 Fragments arranged by size: 4026 2007 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3771 1163 Size: 573 2035 3921 Fragments arranged by size: 3921 2035 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 3979 4740 5768 1540 Size: 275 186 106 1872 761 1028 2301 Fragments arranged by size: 2301 1872 1028 761 275 186 106 BsiHKAI G_wGCw'C Cuts at: 3318 3402 3648 4590 4675 5746 3318 Size: 84 246 942 85 1071 4101 Fragments arranged by size: 4101 1071 942 246 85 84 BsmI GAATG_Cn' Cuts at: 2707 2933 3207 3711 4040 4133 2707 Size: 226 274 504 329 93 5103 Fragments arranged by size: 5103 504 329 274 226 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2229 2454 2792 3318 3402 3553 Size: 399 326 113 225 338 526 84 151 Cuts at: 3553 3648 3848 4590 4675 5746 1391 Size: 95 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 338 326 225 200 151 113 95 85 84 BspEI T'CCGG_A Cuts at: 6192 6192 Size: 6529 BspHI T'CATG_A Cuts at: 3253 4418 6148 3253 Size: 1165 1730 3634 Fragments arranged by size: 3634 1730 1165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2158 2903 3884 844 Size: 1314 745 981 3489 Fragments arranged by size: 3489 1314 981 745 BsrBI CCG'CTC Cuts at: 1394 1823 2054 2205 2494 3043 4416 1394 Size: 429 231 151 289 549 1373 3507 Fragments arranged by size: 3507 1373 549 429 289 231 151 BsrDI GCAATG_nn' Cuts at: 65 1248 5024 5198 65 Size: 1183 3776 174 1396 Fragments arranged by size: 3776 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2102 5173 5360 1700 Size: 110 232 60 3071 187 2869 Fragments arranged by size: 3071 2869 232 187 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6529 BssSI C'ACGA_G Cuts at: 4589 5601 4589 Size: 1012 5517 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 794 Size: 1469 5060 BstBI TT'CG_AA Cuts at: 5333 5333 Size: 6529 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6529 BstXI CCAn_nnnn'nTGG Cuts at: 3131 3131 Size: 6529 BstYI r'GATC_y Cuts at: 1972 2030 2292 2590 3859 4226 4611 4628 6080 Size: 58 262 298 1269 367 385 17 1452 Cuts at: 6080 6166 6178 6524 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1269 385 367 346 298 262 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 3233 3755 3233 Size: 522 6007 BtgI C'CryG_G Cuts at: 513 1065 2072 2107 2189 3054 513 Size: 552 1007 35 82 865 3988 Fragments arranged by size: 3988 1007 865 552 82 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 487 Size: 683 56 1508 4282 Fragments arranged by size: 4282 1508 683 56 BtsI GCAGTG_nn' Cuts at: 3113 3155 4045 4839 4859 3113 Size: 42 890 794 20 4783 Fragments arranged by size: 4783 890 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6494 DraI TTT'AAA Cuts at: 3833 3833 Size: 6529 DraIII CAC_nnn'GTG Cuts at: 2907 3142 2907 Size: 235 6294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 809 Size: 507 4220 1802 Fragments arranged by size: 4220 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2104 3057 Size: 54 1425 50 275 18 210 64 953 Cuts at: 3057 3976 4865 6494 8 Size: 919 889 1629 43 Fragments arranged by size: 1629 1425 953 919 889 275 210 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2104 3976 1537 Size: 275 292 1872 4090 Fragments arranged by size: 4090 1872 292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4459 1353 Size: 3106 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3572 5116 5490 5636 387 Size: 1064 138 1983 1544 374 146 1280 Fragments arranged by size: 1983 1544 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3316 3400 3316 Size: 84 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2814 2933 3098 3260 3332 3340 4592 Size: 393 1182 119 165 162 72 8 1252 Cuts at: 4592 5189 5976 6244 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1252 1182 787 597 393 268 165 162 119 72 8 EcoNI CCTnn'n_nnAGG Cuts at: 2851 3692 2851 Size: 841 5688 EcoO109I rG'GnC_Cy Cuts at: 2112 2113 2170 2488 2856 2892 3169 2112 Size: 1 57 318 368 36 277 5472 Fragments arranged by size: 5472 368 318 277 57 36 1 EcoRI G'AATT_C Cuts at: 3305 3838 4216 3305 Size: 533 378 5618 Fragments arranged by size: 5618 533 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3401 3401 Size: 6529 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 2155 5676 1678 Size: 477 3521 2531 Fragments arranged by size: 3521 2531 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2284 2316 2441 Size: 32 135 32 617 32 63 32 125 Cuts at: 2441 2473 3283 3315 5170 5202 5244 5276 1373 Size: 32 810 32 1855 32 42 32 2626 Fragments arranged by size: 2626 1855 810 617 135 125 63 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4119 669 Size: 988 281 1935 246 3079 Fragments arranged by size: 3079 1935 988 281 246 HindIII A'AGCT_T Cuts at: 748 2610 3107 748 Size: 1862 497 4170 Fragments arranged by size: 4170 1862 497 HpaI GTT'AAC Cuts at: 4119 4119 Size: 6529 KasI G'GCGC_C Cuts at: 2151 2151 Size: 6529 KpnI G_GTAC'C Cuts at: 3854 3854 Size: 6529 MfeI C'AATT_G Cuts at: 4128 4128 Size: 6529 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5643 5827 1305 Size: 4338 184 2007 Fragments arranged by size: 4338 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4707 Fragments arranged by size: 4707 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4487 4846 5005 518 Size: 648 3321 359 159 2042 Fragments arranged by size: 3321 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2109 2191 3945 4622 5770 6015 1606 Size: 468 35 82 1754 677 1148 245 2120 Fragments arranged by size: 2120 1754 1148 677 468 245 82 35 NaeI GCC'GGC Cuts at: 2044 2104 2044 Size: 60 6469 NarI GG'CG_CC Cuts at: 2152 2152 Size: 6529 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5977 NdeI CA'TA_TG Cuts at: 387 3805 387 Size: 3418 3111 NgoMIV G'CCGG_C Cuts at: 2042 2102 2042 Size: 60 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NspI r_CATG'y Cuts at: 1132 1229 3887 5432 1132 Size: 97 2658 1545 2229 Fragments arranged by size: 2658 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6529 PciI A'CATG_T Cuts at: 5428 5428 Size: 6529 PflMI CCAn_nnn'nTGG Cuts at: 1108 2956 1108 Size: 1848 4681 PfoI T'CCnGG_A Cuts at: 3309 3309 Size: 6529 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2488 2856 2892 3169 2170 Size: 318 368 36 277 5530 Fragments arranged by size: 5530 368 318 277 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 3413 4099 3413 Size: 686 5843 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 6529 PstI C_TGCA'G Cuts at: 830 3202 3768 3881 830 Size: 2372 566 113 3478 Fragments arranged by size: 3478 2372 566 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 SacI G_AGCT'C Cuts at: 3318 3402 3318 Size: 84 6445 SacII CC_GC'GG Cuts at: 2075 2110 2192 2075 Size: 35 82 6412 Fragments arranged by size: 6412 82 35 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SanDI GG'GwC_CC Cuts at: 3169 3169 Size: 6529 SbfI CC_TGCA'GG Cuts at: 3881 3881 Size: 6529 ScaI AGT'ACT Cuts at: 4777 6489 4777 Size: 1712 4817 SfcI C'TryA_G Cuts at: 826 1045 2296 3198 3764 3877 5012 5693 5884 Size: 219 1251 902 566 113 1135 681 191 Cuts at: 5884 826 Size: 1471 Fragments arranged by size: 1471 1251 1135 902 681 566 219 191 113 SfoI GGC'GCC Cuts at: 2153 2153 Size: 6529 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6529 SmaI CCC'GGG Cuts at: 1872 3856 1872 Size: 1984 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2049 2392 3069 4633 5534 5796 Size: 197 926 106 343 677 1564 901 262 Cuts at: 5796 820 Size: 1553 Fragments arranged by size: 1564 1553 926 901 677 343 262 197 106 SnaBI TAC'GTA Cuts at: 493 4320 493 Size: 3827 2702 SpeI A'CTAG_T Cuts at: 152 2060 152 Size: 1908 4621 SphI G_CATG'C Cuts at: 1132 3887 1132 Size: 2755 3774 SspI AAT'ATT Cuts at: 5 52 4453 5 Size: 47 4401 2081 Fragments arranged by size: 4401 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2584 3968 513 Size: 552 1519 1384 3074 Fragments arranged by size: 3074 1519 1384 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4556 4573 4726 4911 5356 325 Size: 1255 2976 17 153 185 445 1498 Fragments arranged by size: 2976 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2683 3630 4775 Size: 274 80 33 51 1443 706 947 1145 Cuts at: 4775 6487 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1145 947 706 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3105 3126 3137 3158 6489 6521 3105 Size: 21 11 21 3331 32 3113 Fragments arranged by size: 3331 3113 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6529 XbaI T'CTAG_A Cuts at: 3865 3865 Size: 6529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3061 3061 Size: 6529 XhoI C'TCGA_G Cuts at: 1943 2049 1943 Size: 106 6423 XmaI C'CCGG_G Cuts at: 1870 3854 1870 Size: 1984 4545 XmnI GAAnn'nnTTC Cuts at: 2540 3381 4658 2540 Size: 841 1277 4411 Fragments arranged by size: 4411 1277 841 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4337 276 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI