################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BbrPI BbsI BcgI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FspI HgaI HindIII HpaI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 10 GGCGCC G[GA]^CG[CT]C AflIII 717 ACATGT A^C[GA][CT]GT AhdI 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 677 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 179 AGCGCT AGC^GCT AspEI 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3 GGATCC G^GATCC BanI 10 GGCGCC G^G[CT][GA]CC BanII 57 GGGCTC G[GA]GC[CT]^C BbeI 10 GGCGCC GGCGC^C BbvCI 323 CCTCAGC CCTCAGC(-5/-2) BciVI 659 GTATCC GTATCC(6/5) BglI 102 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 281 AGATCT A^GATCT BmyI 57 GGGCTC G[AGT]GC[ACT]^C BsaHI 10 GGCGCC G[GA]^CG[CT]C BsiEI 113 CGGCCG CG[GA][CT]^CG BsmAI 382 GAGAC GTCTC(1/5) BsmFI 287 GGGAC GGGAC(10/14) Bsp1286I 57 GGGCTC G[AGT]GC[ACT]^C BspLU11I 717 ACATGT A^CATGT BsrFI 111 GCCGGC [GA]^CCGG[CT] BsrI 532 ACTGG ACTGG(1/-1) BstXI 167 CCAGCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 111 GCCGGC [GA]^CCGG[CT] EagI 113 CGGCCG C^GGCCG Eam1105I 713 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 113 CGGCCG C^GGCCG Eco47III 179 AGCGCT AGC^GCT Eco52I 113 CGGCCG C^GGCCG EcoRV 367 GATATC GAT^ATC FseI 110 GGCCGGCC GGCCGG^CC Hin1I 10 GGCGCC G[GA]^CG[CT]C HincII 229 GTTGAC GT[CT]^[GA]AC HindII 229 GTTGAC GT[CT]^[GA]AC HinfI 308 GATTC G^A[ACGT]TC KasI 10 GGCGCC G^GCGCC KspI 150 CCGCGG CCGC^GG MaeIII 669 GTTAC ^GT[ACGT]AC MseI 637 TTAA T^TAA MslI 531 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 150 CCGCGG C[AC]G^C[GT]G NaeI 111 GCCGGC GCC^GGC NarI 10 GGCGCC GG^CGCC NgoMIV 111 GCCGGC G^CCGGC NlaIII 718 CATG CATG^ NspI 717 ACATGT [GA]CATG^[CT] PstI 19 CTGCAG CTGCA^G SacII 150 CCGCGG CCGC^GG SfoI 10 GGCGCC GGC^GCC StuI 206 AGGCCT AGG^CCT TaqI 602 TCGA T^CGA TfiI 308 GATTC G^A[AT]TC Tru9I 637 TTAA T^TAA Tsp509I 270 AATT ^AATT TspRI 531 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 602 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BsiYI 105 CCCCAGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 150 CCGCGGCGTGG BslI 105 CCCCAGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 150 CCGCGGCGTGG BspMI 129 GCAGGT ACCTGC(4/8) 295 GCAGGT BstF5I 407 GGATG GGATG(2/0) 536 GGATG BstYI 3 GGATCC [GA]^GATC[CT] 281 AGATCT Cfr13I 29 GGGCC G^G[ACGT]CC 84 GGGCC DsaI 150 CCGCGG C^C[GA][CT]GG 221 CCGTGG EaeI 78 CGGCCA [CT]^GGCC[GA] 113 CGGCCG EcoT14I 344 CCAAGG C^C[AT][AT]GG 560 CCAAGG FokI 407 GGATG GGATG(9/13) 536 GGATG HphI 185 TCACC GGTGA(8/7) 494 TCACC MboII 411 GAAGA GAAGA(8/7) 436 GAAGA MflI 3 GGATCC [GA]^GATC[CT] 281 AGATCT Sau96I 29 GGGCC G^G[ACGT]CC 84 GGGCC SfcI 19 CTGCAG C^T[GA][CT]AG 248 CTGTAG StyI 344 CCAAGG C^C[AT][AT]GG 560 CCAAGG XhoII 3 GGATCC [GA]^GATC[CT] 281 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BcnI BsaJI BssKI BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI ScrFI SfaNI TseI